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ACTIVE MOTIF910
ANTIBODIESAPPLICATIONISOTYPEREACTIVITYMODIFICATIONPURIFICATION Active Motif offers research kits, assays and biocomputing systems that help researchers study the function, regulation and interactions between genes, proteins and metabolic pathways. NUCLEAR EXTRACT KIT (NUCLEAR, CYTOPLASMIC OR WHOLE-CELL Nuclear Extract Kit Overview. The Nuclear Extract Kit isolates high-quality nuclear, cytoplasmic or whole-cell extract from mammalian cell or tissue samples. This eliminates the need for optimizing reagents and protocols, and ensures high yields every time. More importantly, standardizing your extraction procedure with our high-quality reagentsATAC-SEQ KIT
ATAC-Seq Kit Overview. ATAC-Seq is a rapid assay that allows analysis of epigenetic profiles across the genome by identification of regions that have open or accessible chromatin states. Because of the assay’s speed, simplicity, and applicability to a wide range of sample types, ATAC-Seq has become a commonly-used epigenetic assay,and it can
CHROMATIN IMMUNOPRECIPITATION (CHROMATIN IP) CHIP-IT ChIP-IT ® Express Overview. Chromatin Immunoprecipitation (ChIP) can be technically challenging and yield results that are difficult to interpret. Active Motif's ChIP-IT ® Express Kits eliminate some of these challenges by providing a streamlined protocol using protein G-coated magnetic beads, making it possible to perform ChIP in just 1 day. Kits that use either sonication or enzymatic UNIVERSAL MAGNETIC CO-IP KIT (CO-IMMUNOPRECIPITATION OF The Universal Magnetic Co-IP Kit was used to make nuclear extract from MCF-7 cells that had been induced 1 hour with 10 nM Estradiol. IP was performed on 300 µg samples using 2 µg of SRC-1 pAb, ERα pAb and rabbit IgG (as a negative control). Western blot was then performed using the ERα pAb on 10 µg Input Extract, SRC-1 IP, ERα IP and the WHAT IS ATAC-SEQ & HOW DOES IT WORK? The ATAC-Seq method was just published in 2013 and it has already become one of the most common and powerful ways to study chromatin states on a genome-wide level. This article covers what ATAC-Seq is and how to use it in your research. PARP-1 N-TERMINAL ANTIBODY (PAB) PARP-1 N-terminal ( ADPRT) encodes a chromatin-associated enzyme, poly (ADP-ribosyl)transferase, that modifies various nuclear proteins by poly (ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation and tumor transformation. RE-CHIP-IT® CHROMATIN IMMUNOPRECIPITATION (CHROMATIN IP Re-ChIP (aka Sequential ChIP, Chromatin Re-IP and ChIP Re-ChIP) is a relatively new technique that enables sequential chromatin immunoprecipitations to be performed using two different antibodies so that you can assay for the simultaneous presence of two proteins or distinct histone modifications at the same genomic region of interest. DAX-1 / NR0B1 ANTIBODY (1DA-2F4): WB, IHC, IF/ICC Background. DAX-1 (DSS-AHC critical region on the X chromosome protein 1, NR0B1, Nuclear receptor subfamily 0 group B member 1) is an orphan nuclear receptor and a component of a cascade required for the development of the hypothalamic-pituitary-adrenal-gonadal axis.DAX-1 / NR0B1 inhibits the transcriptional activity of other nuclear receptors through heterodimeric interactions. 5-HYDROXYMETHYLCYTOSINE (5-HMC) ANTIBODY (PAB) 5-Hydroxymethylcytosine (5-hmC, 5-hydroxymethylcytidine) antibody tested by Methyl DNA immunoprecipitation. DNA (25 pg) derived from the promoter of the APC gene was spiked into 500 ng of human genomic DNA and subjected to the MeDIP procedure using 1 μl of 5-Hydroxymethylcytidine antibody (5hmC, maroon bars) or 1 μl of control rabbit IgG (IgG, blue bars).ACTIVE MOTIF910
ANTIBODIESAPPLICATIONISOTYPEREACTIVITYMODIFICATIONPURIFICATION Active Motif offers research kits, assays and biocomputing systems that help researchers study the function, regulation and interactions between genes, proteins and metabolic pathways. NUCLEAR EXTRACT KIT (NUCLEAR, CYTOPLASMIC OR WHOLE-CELL Nuclear Extract Kit Overview. The Nuclear Extract Kit isolates high-quality nuclear, cytoplasmic or whole-cell extract from mammalian cell or tissue samples. This eliminates the need for optimizing reagents and protocols, and ensures high yields every time. More importantly, standardizing your extraction procedure with our high-quality reagentsATAC-SEQ KIT
ATAC-Seq Kit Overview. ATAC-Seq is a rapid assay that allows analysis of epigenetic profiles across the genome by identification of regions that have open or accessible chromatin states. Because of the assay’s speed, simplicity, and applicability to a wide range of sample types, ATAC-Seq has become a commonly-used epigenetic assay,and it can
CHROMATIN IMMUNOPRECIPITATION (CHROMATIN IP) CHIP-IT ChIP-IT ® Express Overview. Chromatin Immunoprecipitation (ChIP) can be technically challenging and yield results that are difficult to interpret. Active Motif's ChIP-IT ® Express Kits eliminate some of these challenges by providing a streamlined protocol using protein G-coated magnetic beads, making it possible to perform ChIP in just 1 day. Kits that use either sonication or enzymatic UNIVERSAL MAGNETIC CO-IP KIT (CO-IMMUNOPRECIPITATION OF The Universal Magnetic Co-IP Kit was used to make nuclear extract from MCF-7 cells that had been induced 1 hour with 10 nM Estradiol. IP was performed on 300 µg samples using 2 µg of SRC-1 pAb, ERα pAb and rabbit IgG (as a negative control). Western blot was then performed using the ERα pAb on 10 µg Input Extract, SRC-1 IP, ERα IP and the WHAT IS ATAC-SEQ & HOW DOES IT WORK? The ATAC-Seq method was just published in 2013 and it has already become one of the most common and powerful ways to study chromatin states on a genome-wide level. This article covers what ATAC-Seq is and how to use it in your research. PARP-1 N-TERMINAL ANTIBODY (PAB) PARP-1 N-terminal ( ADPRT) encodes a chromatin-associated enzyme, poly (ADP-ribosyl)transferase, that modifies various nuclear proteins by poly (ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation and tumor transformation. RE-CHIP-IT® CHROMATIN IMMUNOPRECIPITATION (CHROMATIN IP Re-ChIP (aka Sequential ChIP, Chromatin Re-IP and ChIP Re-ChIP) is a relatively new technique that enables sequential chromatin immunoprecipitations to be performed using two different antibodies so that you can assay for the simultaneous presence of two proteins or distinct histone modifications at the same genomic region of interest. DAX-1 / NR0B1 ANTIBODY (1DA-2F4): WB, IHC, IF/ICC Background. DAX-1 (DSS-AHC critical region on the X chromosome protein 1, NR0B1, Nuclear receptor subfamily 0 group B member 1) is an orphan nuclear receptor and a component of a cascade required for the development of the hypothalamic-pituitary-adrenal-gonadal axis.DAX-1 / NR0B1 inhibits the transcriptional activity of other nuclear receptors through heterodimeric interactions. 5-HYDROXYMETHYLCYTOSINE (5-HMC) ANTIBODY (PAB) 5-Hydroxymethylcytosine (5-hmC, 5-hydroxymethylcytidine) antibody tested by Methyl DNA immunoprecipitation. DNA (25 pg) derived from the promoter of the APC gene was spiked into 500 ng of human genomic DNA and subjected to the MeDIP procedure using 1 μl of 5-Hydroxymethylcytidine antibody (5hmC, maroon bars) or 1 μl of control rabbit IgG (IgG, blue bars).EPIGENETICS EBOOKS
The link between epigenetics and cancer has been demonstrated through the identification of DNA methylation biomarkers associated with many different types of cancer as well as mutations in epigenetic enzymes that contribute to tumorigenesis, metastasis, and cancer progression. This DNA Methylation and Cancer eBook provides a comprehensive in RECOMBINANT SARS-COV-2 NSP16 PROTEIN, 1 MG SIZE Recombinant SARS-CoV-2 NSP16 protein was expressed in E.coli cells as the full length protein (accession number YP_009725311.1) with a C-terminal 6×His tag. The predicted molecular weight of the protein is 34.5 kDa. Recombinant SARS-CoV-2 NSP16 protein PIXUL™ MULTI-SAMPLE SONICATOR PIXUL Multi-Sample Sonicator Highlights: Process 1-96 samples in parallel to avoid sample shearing bottlenecks in NGS applications; Up to 12 unique sonication conditions can be run on a single 96-well plate, allowing simultaneous processing of multiple sample types and rapid optimization of conditions for difficult samples PARP-1 N-TERMINAL ANTIBODY (PAB) PARP-1 N-terminal ( ADPRT) encodes a chromatin-associated enzyme, poly (ADP-ribosyl)transferase, that modifies various nuclear proteins by poly (ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation and tumor transformation. CHROMATIN IMMUNOPRECIPITATION (CHROMATIN IP) CHIP-IT ChIP-IT ® Express Overview. Chromatin Immunoprecipitation (ChIP) can be technically challenging and yield results that are difficult to interpret. Active Motif's ChIP-IT ® Express Kits eliminate some of these challenges by providing a streamlined protocol using protein G-coated magnetic beads, making it possible to perform ChIP in just 1 day. Kits that use either sonication or enzymatic SORTAG-IT™ LABELING KITS FOR DIRECT CONJUGATION OF A Sortag-IT™ Labeling Kit and Sortase A5 Labeling Scheme. Sortase A belongs to a family of transpeptidases found in Gram-positive bacteria that catalyze the attachment of poly-Glycine (G) n labels to the sortase recognition sequence. The Sortag-IT Labeling Kits use Active Motif's Sortase A5 pentamutant from Staphylococcus aureus that is significantly more active than wild-type Sortase to CELL-FREE DNA EXTRACTION KIT The Cell-Free DNA (cfDNA) Purification Kit is designed to efficiently isolate cell-free DNA from human plasma and serum samples. Using Active Motif's fast, magnetic bead-based protocol, it is easy to isolate and concentrate cell-free DNA from fresh or frozen samples. The profile below demonstrates total elimination of high molecularweight
5-HYDROXYMETHYLCYTOSINE (5-HMC) ANTIBODY (PAB) 5-Hydroxymethylcytosine (5-hmC, 5-hydroxymethylcytidine) antibody tested by Methyl DNA immunoprecipitation. DNA (25 pg) derived from the promoter of the APC gene was spiked into 500 ng of human genomic DNA and subjected to the MeDIP procedure using 1 μl of 5-Hydroxymethylcytidine antibody (5hmC, maroon bars) or 1 μl of control rabbit IgG (IgG, blue bars). HTZ1 / HISTONE H2A.Z ANTIBODY (PAB) (H2AZ) Background. Htz1 is the S. cerevesiae homolog of histone H2AZ and is involved in transcriptional regulation and chromosome architecture.. Positive Control. Whole-cell extracts from S. pombe can be used as a positive control for Htz1 antibody. RECOMBINANT PRMT5/MEP50 COMPLEX, ACTIVITY-TESTED Protein Details. Recombinant PRMT5 / MEP50 Complex that includes full-length PRMT5 (accession numbers NP_006100.2) with an N-terminal FLAG tag and full-length MEP50 (accession numbers NP_077007.1) without tag was expressed in a baculovirus expression system. The molecular weights of PRMT5 and MEP50 are 74 kDa and 36.7 kDa, respectively.ACTIVE MOTIF910
ANTIBODIESAPPLICATIONISOTYPEREACTIVITYMODIFICATIONPURIFICATION Active Motif offers research kits, assays and biocomputing systems that help researchers study the function, regulation and interactions between genes, proteins and metabolic pathways. NUCLEAR EXTRACT KIT (NUCLEAR, CYTOPLASMIC OR WHOLE-CELL Nuclear Extract Kit Overview. The Nuclear Extract Kit isolates high-quality nuclear, cytoplasmic or whole-cell extract from mammalian cell or tissue samples. This eliminates the need for optimizing reagents and protocols, and ensures high yields every time. More importantly, standardizing your extraction procedure with our high-quality reagents CELL-FREE DNA EXTRACTION KIT The Cell-Free DNA (cfDNA) Purification Kit is designed to efficiently isolate cell-free DNA from human plasma and serum samples. Using Active Motif's fast, magnetic bead-based protocol, it is easy to isolate and concentrate cell-free DNA from fresh or frozen samples. The profile below demonstrates total elimination of high molecularweight
UNIVERSAL MAGNETIC CO-IP KIT (CO-IMMUNOPRECIPITATION OF The Universal Magnetic Co-IP Kit was used to make nuclear extract from MCF-7 cells that had been induced 1 hour with 10 nM Estradiol. IP was performed on 300 µg samples using 2 µg of SRC-1 pAb, ERα pAb and rabbit IgG (as a negative control). Western blot was then performed using the ERα pAb on 10 µg Input Extract, SRC-1 IP, ERα IP and the WHAT IS ATAC-SEQ & HOW DOES IT WORK? The ATAC-Seq method was just published in 2013 and it has already become one of the most common and powerful ways to study chromatin states on a genome-wide level. This article covers what ATAC-Seq is and how to use it in your research. PIXUL™ MULTI-SAMPLE SONICATORATAC-SEQ KIT
ATAC-Seq Kit Overview. ATAC-Seq is a rapid assay that allows analysis of epigenetic profiles across the genome by identification of regions that have open or accessible chromatin states. Because of the assay’s speed, simplicity, and applicability to a wide range of sample types, ATAC-Seq has become a commonly-used epigenetic assay,and it can
DAX-1 / NR0B1 ANTIBODY (1DA-2F4): WB, IHC, IF/ICC Background. DAX-1 (DSS-AHC critical region on the X chromosome protein 1, NR0B1, Nuclear receptor subfamily 0 group B member 1) is an orphan nuclear receptor and a component of a cascade required for the development of the hypothalamic-pituitary-adrenal-gonadal axis.DAX-1 / NR0B1 inhibits the transcriptional activity of other nuclear receptors through heterodimeric interactions. RE-CHIP-IT® CHROMATIN IMMUNOPRECIPITATION (CHROMATIN IP Re-ChIP (aka Sequential ChIP, Chromatin Re-IP and ChIP Re-ChIP) is a relatively new technique that enables sequential chromatin immunoprecipitations to be performed using two different antibodies so that you can assay for the simultaneous presence of two proteins or distinct histone modifications at the same genomic region of interest. 5-HYDROXYMETHYLCYTOSINE (5-HMC) ANTIBODY (PAB) 5-Hydroxymethylcytosine (5-hmC, 5-hydroxymethylcytidine) antibody tested by Methyl DNA immunoprecipitation. DNA (25 pg) derived from the promoter of the APC gene was spiked into 500 ng of human genomic DNA and subjected to the MeDIP procedure using 1 μl of 5-Hydroxymethylcytidine antibody (5hmC, maroon bars) or 1 μl of control rabbit IgG (IgG, blue bars).ACTIVE MOTIF910
ANTIBODIESAPPLICATIONISOTYPEREACTIVITYMODIFICATIONPURIFICATION Active Motif offers research kits, assays and biocomputing systems that help researchers study the function, regulation and interactions between genes, proteins and metabolic pathways. NUCLEAR EXTRACT KIT (NUCLEAR, CYTOPLASMIC OR WHOLE-CELL Nuclear Extract Kit Overview. The Nuclear Extract Kit isolates high-quality nuclear, cytoplasmic or whole-cell extract from mammalian cell or tissue samples. This eliminates the need for optimizing reagents and protocols, and ensures high yields every time. More importantly, standardizing your extraction procedure with our high-quality reagents CELL-FREE DNA EXTRACTION KIT The Cell-Free DNA (cfDNA) Purification Kit is designed to efficiently isolate cell-free DNA from human plasma and serum samples. Using Active Motif's fast, magnetic bead-based protocol, it is easy to isolate and concentrate cell-free DNA from fresh or frozen samples. The profile below demonstrates total elimination of high molecularweight
UNIVERSAL MAGNETIC CO-IP KIT (CO-IMMUNOPRECIPITATION OF The Universal Magnetic Co-IP Kit was used to make nuclear extract from MCF-7 cells that had been induced 1 hour with 10 nM Estradiol. IP was performed on 300 µg samples using 2 µg of SRC-1 pAb, ERα pAb and rabbit IgG (as a negative control). Western blot was then performed using the ERα pAb on 10 µg Input Extract, SRC-1 IP, ERα IP and the WHAT IS ATAC-SEQ & HOW DOES IT WORK? The ATAC-Seq method was just published in 2013 and it has already become one of the most common and powerful ways to study chromatin states on a genome-wide level. This article covers what ATAC-Seq is and how to use it in your research. PIXUL™ MULTI-SAMPLE SONICATORATAC-SEQ KIT
ATAC-Seq Kit Overview. ATAC-Seq is a rapid assay that allows analysis of epigenetic profiles across the genome by identification of regions that have open or accessible chromatin states. Because of the assay’s speed, simplicity, and applicability to a wide range of sample types, ATAC-Seq has become a commonly-used epigenetic assay,and it can
DAX-1 / NR0B1 ANTIBODY (1DA-2F4): WB, IHC, IF/ICC Background. DAX-1 (DSS-AHC critical region on the X chromosome protein 1, NR0B1, Nuclear receptor subfamily 0 group B member 1) is an orphan nuclear receptor and a component of a cascade required for the development of the hypothalamic-pituitary-adrenal-gonadal axis.DAX-1 / NR0B1 inhibits the transcriptional activity of other nuclear receptors through heterodimeric interactions. RE-CHIP-IT® CHROMATIN IMMUNOPRECIPITATION (CHROMATIN IP Re-ChIP (aka Sequential ChIP, Chromatin Re-IP and ChIP Re-ChIP) is a relatively new technique that enables sequential chromatin immunoprecipitations to be performed using two different antibodies so that you can assay for the simultaneous presence of two proteins or distinct histone modifications at the same genomic region of interest. 5-HYDROXYMETHYLCYTOSINE (5-HMC) ANTIBODY (PAB) 5-Hydroxymethylcytosine (5-hmC, 5-hydroxymethylcytidine) antibody tested by Methyl DNA immunoprecipitation. DNA (25 pg) derived from the promoter of the APC gene was spiked into 500 ng of human genomic DNA and subjected to the MeDIP procedure using 1 μl of 5-Hydroxymethylcytidine antibody (5hmC, maroon bars) or 1 μl of control rabbit IgG (IgG, blue bars). RECOMBINANT SARS-COV-2 NSP16 PROTEIN, 1 MG SIZE Recombinant SARS-CoV-2 NSP16 protein was expressed in E.coli cells as the full length protein (accession number YP_009725311.1) with a C-terminal 6×His tag. The predicted molecular weight of the protein is 34.5 kDa. Recombinant SARS-CoV-2 NSP16 proteinEPIGENETICS EBOOKS
The link between epigenetics and cancer has been demonstrated through the identification of DNA methylation biomarkers associated with many different types of cancer as well as mutations in epigenetic enzymes that contribute to tumorigenesis, metastasis, and cancer progression. This DNA Methylation and Cancer eBook provides a comprehensive in PIXUL™ MULTI-SAMPLE SONICATOR PIXUL Multi-Sample Sonicator Highlights: Process 1-96 samples in parallel to avoid sample shearing bottlenecks in NGS applications; Up to 12 unique sonication conditions can be run on a single 96-well plate, allowing simultaneous processing of multiple sample types and rapid optimization of conditions for difficult samples CHROMATIN IMMUNOPRECIPITATION (CHROMATIN IP) CHIP-IT ChIP-IT ® Express Overview. Chromatin Immunoprecipitation (ChIP) can be technically challenging and yield results that are difficult to interpret. Active Motif's ChIP-IT ® Express Kits eliminate some of these challenges by providing a streamlined protocol using protein G-coated magnetic beads, making it possible to perform ChIP in just 1 day. Kits that use either sonication or enzymatic TRANSAM TRANSCRIPTION FACTOR ACTIVATION ASSAYS TransAM ® kits are sensitive, non-radioactive transcription factor ELISA-based assays that facilitate the study of transcription factor activation in mammalian tissue and cell extracts. These optimized assays are available for many different transcription factors, outlined in the table below.. View Available Transcription Factors. How TransAM Transcription Factor ELISAs Work SORTAG-IT™ LABELING KITS FOR DIRECT CONJUGATION OF A Sortag-IT™ Labeling Kit and Sortase A5 Labeling Scheme. Sortase A belongs to a family of transpeptidases found in Gram-positive bacteria that catalyze the attachment of poly-Glycine (G) n labels to the sortase recognition sequence. The Sortag-IT Labeling Kits use Active Motif's Sortase A5 pentamutant from Staphylococcus aureus that is significantly more active than wild-type Sortase to QUANTITATIVELY MEASURE NF-KB ACTIVATION WITH TRANSAM ASSAYS TransAM NF-κB kits are DNA-binding ELISAs that provide everything needed to study NF-κB activation in biological samples, including a positive control extract. These assays are available to quantitatively analyze either multiple NF-κB family subunits (p50, p52, p65, c-Rel, and RelB) or the specific NF-κB subunits p50, p52, and p65. 5-HYDROXYMETHYLCYTOSINE (5-HMC) ANTIBODY (PAB) 5-Hydroxymethylcytosine (5-hmC, 5-hydroxymethylcytidine) antibody tested by Methyl DNA immunoprecipitation. DNA (25 pg) derived from the promoter of the APC gene was spiked into 500 ng of human genomic DNA and subjected to the MeDIP procedure using 1 μl of 5-Hydroxymethylcytidine antibody (5hmC, maroon bars) or 1 μl of control rabbit IgG (IgG, blue bars). RECOMBINANT SMARCA2 PROTEIN (AA1367-1511), 1 MG The peptide corresponding to amino acids 1367-1511 that contains the bromodomain sequences of SMARCA2 / BRM (accession number NP_003061.3) was expressed in E. coli and contains an N-terminal His-Tag and C-terminal FLAG-Tag with an observed molecular weight of 22.96 kDa. It shows binding specificity for acetylated H3K9, H3K14, H3K9/14, H4K8 HTZ1 / HISTONE H2A.Z ANTIBODY (PAB) (H2AZ) Background. Htz1 is the S. cerevesiae homolog of histone H2AZ and is involved in transcriptional regulation and chromosome architecture.. Positive Control. Whole-cell extracts from S. pombe can be used as a positive control for Htz1 antibody.ACTIVE MOTIF910
ANTIBODIESAPPLICATIONISOTYPEREACTIVITYMODIFICATIONPURIFICATION Active Motif offers research kits, assays and biocomputing systems that help researchers study the function, regulation and interactions between genes, proteins and metabolic pathways. RECOMBINANT SARS-COV-2 NSP16 PROTEIN, 1 MG SIZESEE MORE ONACTIVEMOTIF.COM
RECOMBINANT SRC PROTEIN, ACTIVITY-TESTED, 1 MGSEE MORE ONACTIVEMOTIF.COM
UNIVERSAL MAGNETIC CO-IP KIT (CO-IMMUNOPRECIPITATION OFAMAZON MAGNETIC BEADSMAGNETIC BEADS FOR BRACELETSMAGNETIC BEADS FOR KIDSMAGNETIC BEADSFOR SALE
The Universal Magnetic Co-IP Kit was used to make nuclear extract from MCF-7 cells that had been induced 1 hour with 10 nM Estradiol. IP was performed on 300 µg samples using 2 µg of SRC-1 pAb, ERα pAb and rabbit IgG (as a negative control). Western blot was then performed using the ERα pAb on 10 µg Input Extract, SRC-1 IP, ERα IP and the CHROMATIN IMMUNOPRECIPITATION (CHROMATIN IP) CHIP-ITACTIVE MOTIF CHIP KITACTIVE MOTIF CHIP ChIP-IT ® Express Overview. Chromatin Immunoprecipitation (ChIP) can be technically challenging and yield results that are difficult to interpret. Active Motif's ChIP-IT ® Express Kits eliminate some of these challenges by providing a streamlined protocol using protein G-coated magnetic beads, making it possible to perform ChIP in just 1 day. Kits that use either sonication or enzymatic SORTAG-IT™ LABELING KITS FOR DIRECT CONJUGATION OF A Sortag-IT™ Labeling Kit and Sortase A5 Labeling Scheme. Sortase A belongs to a family of transpeptidases found in Gram-positive bacteria that catalyze the attachment of poly-Glycine (G) n labels to the sortase recognition sequence. The Sortag-IT Labeling Kits use Active Motif's Sortase A5 pentamutant from Staphylococcus aureus that is significantly more active than wild-type Sortase to PARP-1 N-TERMINAL ANTIBODY (PAB) PARP-1 N-terminal ( ADPRT) encodes a chromatin-associated enzyme, poly (ADP-ribosyl)transferase, that modifies various nuclear proteins by poly (ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation and tumor transformation. DAX-1 / NR0B1 ANTIBODY (1DA-2F4): WB, IHC, IF/ICC Background. DAX-1 (DSS-AHC critical region on the X chromosome protein 1, NR0B1, Nuclear receptor subfamily 0 group B member 1) is an orphan nuclear receptor and a component of a cascade required for the development of the hypothalamic-pituitary-adrenal-gonadal axis.DAX-1 / NR0B1 inhibits the transcriptional activity of other nuclear receptors through heterodimeric interactions. 5-HYDROXYMETHYLCYTOSINE (5-HMC) ANTIBODY (PAB) 5-Hydroxymethylcytosine (5-hmC, 5-hydroxymethylcytidine) antibody tested by Methyl DNA immunoprecipitation. DNA (25 pg) derived from the promoter of the APC gene was spiked into 500 ng of human genomic DNA and subjected to the MeDIP procedure using 1 μl of 5-Hydroxymethylcytidine antibody (5hmC, maroon bars) or 1 μl of control rabbit IgG (IgG, blue bars). 5-MC ANTIBODY, CLONE 33D3, FOR MEDIP, IHC, DOT BLOT 5-Methylcytosine antibody (mAb) (Clone 33D3) tested by Methyl-DNA Immunoprecipitation (MeDIP). Human DNA (500 ng) digested with Mse I was subjected to MeDIP and then analyzed by quantitative real-time PCR with primers specific for genes that are normally methylated (Xist, NYESO and SNRPN) or unmethylated (APC and p48). The MeDIP'd DNA (enriched DNA) was plotted.ACTIVE MOTIF910
ANTIBODIESAPPLICATIONISOTYPEREACTIVITYMODIFICATIONPURIFICATION Active Motif offers research kits, assays and biocomputing systems that help researchers study the function, regulation and interactions between genes, proteins and metabolic pathways. RECOMBINANT SARS-COV-2 NSP16 PROTEIN, 1 MG SIZESEE MORE ONACTIVEMOTIF.COM
RECOMBINANT SRC PROTEIN, ACTIVITY-TESTED, 1 MGSEE MORE ONACTIVEMOTIF.COM
UNIVERSAL MAGNETIC CO-IP KIT (CO-IMMUNOPRECIPITATION OFAMAZON MAGNETIC BEADSMAGNETIC BEADS FOR BRACELETSMAGNETIC BEADS FOR KIDSMAGNETIC BEADSFOR SALE
The Universal Magnetic Co-IP Kit was used to make nuclear extract from MCF-7 cells that had been induced 1 hour with 10 nM Estradiol. IP was performed on 300 µg samples using 2 µg of SRC-1 pAb, ERα pAb and rabbit IgG (as a negative control). Western blot was then performed using the ERα pAb on 10 µg Input Extract, SRC-1 IP, ERα IP and the CHROMATIN IMMUNOPRECIPITATION (CHROMATIN IP) CHIP-ITACTIVE MOTIF CHIP KITACTIVE MOTIF CHIP ChIP-IT ® Express Overview. Chromatin Immunoprecipitation (ChIP) can be technically challenging and yield results that are difficult to interpret. Active Motif's ChIP-IT ® Express Kits eliminate some of these challenges by providing a streamlined protocol using protein G-coated magnetic beads, making it possible to perform ChIP in just 1 day. Kits that use either sonication or enzymatic SORTAG-IT™ LABELING KITS FOR DIRECT CONJUGATION OF A Sortag-IT™ Labeling Kit and Sortase A5 Labeling Scheme. Sortase A belongs to a family of transpeptidases found in Gram-positive bacteria that catalyze the attachment of poly-Glycine (G) n labels to the sortase recognition sequence. The Sortag-IT Labeling Kits use Active Motif's Sortase A5 pentamutant from Staphylococcus aureus that is significantly more active than wild-type Sortase to PARP-1 N-TERMINAL ANTIBODY (PAB) PARP-1 N-terminal ( ADPRT) encodes a chromatin-associated enzyme, poly (ADP-ribosyl)transferase, that modifies various nuclear proteins by poly (ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation and tumor transformation. DAX-1 / NR0B1 ANTIBODY (1DA-2F4): WB, IHC, IF/ICC Background. DAX-1 (DSS-AHC critical region on the X chromosome protein 1, NR0B1, Nuclear receptor subfamily 0 group B member 1) is an orphan nuclear receptor and a component of a cascade required for the development of the hypothalamic-pituitary-adrenal-gonadal axis.DAX-1 / NR0B1 inhibits the transcriptional activity of other nuclear receptors through heterodimeric interactions. 5-HYDROXYMETHYLCYTOSINE (5-HMC) ANTIBODY (PAB) 5-Hydroxymethylcytosine (5-hmC, 5-hydroxymethylcytidine) antibody tested by Methyl DNA immunoprecipitation. DNA (25 pg) derived from the promoter of the APC gene was spiked into 500 ng of human genomic DNA and subjected to the MeDIP procedure using 1 μl of 5-Hydroxymethylcytidine antibody (5hmC, maroon bars) or 1 μl of control rabbit IgG (IgG, blue bars). 5-MC ANTIBODY, CLONE 33D3, FOR MEDIP, IHC, DOT BLOT 5-Methylcytosine antibody (mAb) (Clone 33D3) tested by Methyl-DNA Immunoprecipitation (MeDIP). Human DNA (500 ng) digested with Mse I was subjected to MeDIP and then analyzed by quantitative real-time PCR with primers specific for genes that are normally methylated (Xist, NYESO and SNRPN) or unmethylated (APC and p48). The MeDIP'd DNA (enriched DNA) was plotted. RECOMBINANT SARS-COV-2 NSP16 PROTEIN, 1 MG SIZE Recombinant SARS-CoV-2 NSP16 protein was expressed in E.coli cells as the full length protein (accession number YP_009725311.1) with a C-terminal 6×His tag. The predicted molecular weight of the protein is 34.5 kDa. Recombinant SARS-CoV-2 NSP16 protein COMPLETE GUIDE TO SONICATION FOR CHIP January 31, 2020. Chromatin immunoprecipitation (ChIP) is the gold standard method to analyze DNA-binding proteins and their associated DNA sequences. It is widely used to study transcription factors and cofactors as well as histones and histone post-translational modifications. However, ChIP is a multi-step experiment where eachstep has to be
CHROMATIN IMMUNOPRECIPITATION (CHROMATIN IP) CHIP-IT ChIP-IT ® Express Overview. Chromatin Immunoprecipitation (ChIP) can be technically challenging and yield results that are difficult to interpret. Active Motif's ChIP-IT ® Express Kits eliminate some of these challenges by providing a streamlined protocol using protein G-coated magnetic beads, making it possible to perform ChIP in just 1 day. Kits that use either sonication or enzymatic DNASE I TREATMENT KIT The DNase I Treatment Kit utilizes an endonuclease that non-specifically cleaves ssDNA, dsDNA and the DNA strand of RNA:DNA hybrids to improve the purity of your RNA samples. Following DNase I treatment, RNA samples are purified with easy-to-use spin columns. RNA is suitable for use in downstream applications such as RT-qPCR andRNA-seq.
RECOMBINANT HUMAN HDAC6, ACTIVITY-TESTED, 1 MG Protein Details. Recombinant human HDAC6 was expressed in a baculovirus expression system as the full length protein (accession number NP_001308154.1) with a C-terminal FLAG-Tag. The molecular weight of the protein is 135.7 kDa. The recombinant protein is >85% pure by SDS-PAGE. Recombinant HDAC6 protein gel. 5-MC POLYCLONAL ANTIBODY FOR MEDIP, AND DOT BLOT Background. 5-Methylcytosine (5-Methylcytidine) is a modified base that is found in the DNA of plants and vertebrates. DNA methylation is an epigenetic event in which DNA methyltransferases (DNMTs) catalyze the reaction of a methyl group to the fifth carbon of cytosine in a CpG dinucleotide.This modification helps to control gene expression and is also involved in genomic imprinting, while CHARIOT PROTEIN DELIVERY REAGENT (TRANSFECTION Chariot™* is a revolutionary delivery reagent that quickly and efficiently transports biologically active proteins, peptides and antibodies directly into cells. The typical delivery efficiency is 60-95%. Less than two hours after delivery, live cells can be assayed to determine the effects of the introduced material, without the needfor fixing.
HISTONE H3 MONOMETHYL LYS4 ANTIBODY (PAB) Histone H3 monomethyl Lys4 antibody was applied at a dilution of 1:5,000 to Active Motif's MODified™ Histone Peptide Array (Catalog No. 13001). The arrays were scanned with ArrayAnalysis Software 7 and the results plotted. Specificity data is shown for the most reactive peptides and those related to RECOMBINANT PRMT5/MEP50 COMPLEX, ACTIVITY-TESTED Protein Details. Recombinant PRMT5 / MEP50 Complex that includes full-length PRMT5 (accession numbers NP_006100.2) with an N-terminal FLAG tag and full-length MEP50 (accession numbers NP_077007.1) without tag was expressed in a baculovirus expression system. The molecular weights of PRMT5 and MEP50 are 74 kDa and 36.7 kDa, respectively. RECOMBINANT IDO1 PROTEIN, ACTIVITY-TESTED, 1 MG IDO1 (Indoleamine 2,3-dioxygenase 1, also known as IDO, INDO, or IDO-1) is a heme enzyme that catalyzes the first and rate-limiting step in tryptophan catabolism to N-formyl-kynurenine. Tryptophan shortage inhibits T lymphocytes division and accumulation of tryptophan catabolites induces T-cell apoptosis and differentiation ofregulatory T-cells.
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* (21) Fluorescence
* (5) Sample Preparation* (2) Cell Cycle
* (1) Apoptosis
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* (8) Yes
* Absorption Range
* (10) 450-529 nm
* (7) 530-600 nm
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* (1) 350-445 nm
* Emission Range
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* (211) Histone
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* (55) Histone Methyltransferase (HMT)* (27) Kinase
* (24) Histone Deacetylase (HDAC) * (20) Histone Demethylase (HDM)* (19) DNA Damage
* (17) Nuclear Receptor * (12) Histone Acetyltransferase (HAT) * (9) DNA Methyltransferase (DNMT) * (5) Cytosine Oxygenase (TET) * (1) Glucosyltransferase* (1) Growth Factor
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* (66) Biotinylated
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* Research Area
* (396) Chromatin & Histones* (239) Methylation
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* (58) Sample Preparation * (55) Chromatin & Histones * (33) Acetylation & Deacetylation* (25) Methylation
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