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MM.1S | ATCC
The parent cell line, MM.1, was established from peripheral blood of a multiple myeloma patient who had become resistant to steroid-based therapy. The closely related cell line, MM.1R ( ATCC CRL-2975) was also isolated from MM.1, but is resistant to dexamethasone.SUP-T1
The cells express multiple T lineage markers.The cells are E - rosette negative and beta 2 microglobulin positive.The cells are negative for Leu 5 (CD2), sheep erythrocyte receptor, OKT 9 (CD71; transferrin receptor), surface immunoglobulin (sIg -), HLA DR, CALLA (CD10) andTac (CD25).
C2C12 | ATCC
The C2C12 cell line differentiates rapidly, forming contractile myotubes and producing characteristic muscle proteins.Treatment with bone morphogenic protein 2 (BMP-2) cause a shift in the differentiation pathway from myoblastic to osteoblastic.Tested and found negative for ectromelia virus (mousepox).THP-1 | ATCC
The lot specific information (number of cells and expected viability) from the Certificate of Analysis (COA) should be used to calculate the volume of medium necessary to start the THP-1 (ATCC TIB-202) cell culture at the recommended seeding density of 3 XU-937 | ATCC
Studies since 1979 have shown that U-937 cells can be induced to terminal monocytic differentiation by supernatants from human mixed lymphocyte cultures, The cells are negative for immunoglobulin production and Epstein-Barr virus expression. The cells express the Fas antigen, and are sensitive to TNF and anti-Fas antibodies. In 1994, PCR and cytogenetic analyses showed that a number of stocksNK-92® | ATCC
The cell line is dependent on the presence of recombinant Il-2 and a dose as low as 10 U/mL is sufficient to maintain proliferation; cells will die within 72 hours in the absence of IL-2. NK-92® cells have the following characteristics: surface marker positive for CD2, CD7, CD11a, CD28, CD45, CD54 and CD56 bright; surface marker negative for CD1, CD3, CD4, CD5, CD8, CD10, CD14, CD16, CD19MLE 12 | ATCC
Documentation. To download a certificate of analysis for MLE 12 ( CRL-2110 ), enter the lot number exactly as it appears on your product label or packing slip. The certificate of analysis for that lot of MLE 12 ( CRL-2110) is not currently available online. Complete this form to request this certificate of analysis.RWPE-1 | ATCC
In 3-dimensional Matrigel culture, RWPE-1 cells organize into acini and secrete PSA into the lumen when exposed to androgen. RefBello-DeOcampo D, et al. Laminin-1 and alpha6beta1 integrin regulate acinar morphogenesis of normal and malignant human prostate epithelial cells. Prostate 46: 142-153, 2001. PubMed: 11170142 When injected with Matrigel or with stromal cells, into male athymicrodents
NCI-H358
Ultrastructural studies demonstrated the presence of cytoplasmic structures characteristic of Clara cells.The cells expressed protein and RNA of SP-A, the major lung surfactant associated protein.SP-B and SP-C RNA was not expressed.They have a reported colony forming efficiency of 0.83% in soft agarose. ATCC: THE GLOBAL BIORESOURCE CENTER ATCC is a nonprofit organization that collects, stores, and distributes standard reference microorganisms, cell lines, and other materials for research and development.MM.1S | ATCC
The parent cell line, MM.1, was established from peripheral blood of a multiple myeloma patient who had become resistant to steroid-based therapy. The closely related cell line, MM.1R ( ATCC CRL-2975) was also isolated from MM.1, but is resistant to dexamethasone.SUP-T1
The cells express multiple T lineage markers.The cells are E - rosette negative and beta 2 microglobulin positive.The cells are negative for Leu 5 (CD2), sheep erythrocyte receptor, OKT 9 (CD71; transferrin receptor), surface immunoglobulin (sIg -), HLA DR, CALLA (CD10) andTac (CD25).
C2C12 | ATCC
The C2C12 cell line differentiates rapidly, forming contractile myotubes and producing characteristic muscle proteins.Treatment with bone morphogenic protein 2 (BMP-2) cause a shift in the differentiation pathway from myoblastic to osteoblastic.Tested and found negative for ectromelia virus (mousepox).THP-1 | ATCC
The lot specific information (number of cells and expected viability) from the Certificate of Analysis (COA) should be used to calculate the volume of medium necessary to start the THP-1 (ATCC TIB-202) cell culture at the recommended seeding density of 3 XU-937 | ATCC
Studies since 1979 have shown that U-937 cells can be induced to terminal monocytic differentiation by supernatants from human mixed lymphocyte cultures, The cells are negative for immunoglobulin production and Epstein-Barr virus expression. The cells express the Fas antigen, and are sensitive to TNF and anti-Fas antibodies. In 1994, PCR and cytogenetic analyses showed that a number of stocksNK-92® | ATCC
The cell line is dependent on the presence of recombinant Il-2 and a dose as low as 10 U/mL is sufficient to maintain proliferation; cells will die within 72 hours in the absence of IL-2. NK-92® cells have the following characteristics: surface marker positive for CD2, CD7, CD11a, CD28, CD45, CD54 and CD56 bright; surface marker negative for CD1, CD3, CD4, CD5, CD8, CD10, CD14, CD16, CD19MLE 12 | ATCC
Documentation. To download a certificate of analysis for MLE 12 ( CRL-2110 ), enter the lot number exactly as it appears on your product label or packing slip. The certificate of analysis for that lot of MLE 12 ( CRL-2110) is not currently available online. Complete this form to request this certificate of analysis.RWPE-1 | ATCC
In 3-dimensional Matrigel culture, RWPE-1 cells organize into acini and secrete PSA into the lumen when exposed to androgen. RefBello-DeOcampo D, et al. Laminin-1 and alpha6beta1 integrin regulate acinar morphogenesis of normal and malignant human prostate epithelial cells. Prostate 46: 142-153, 2001. PubMed: 11170142 When injected with Matrigel or with stromal cells, into male athymicrodents
NCI-H358
Ultrastructural studies demonstrated the presence of cytoplasmic structures characteristic of Clara cells.The cells expressed protein and RNA of SP-A, the major lung surfactant associated protein.SP-B and SP-C RNA was not expressed.They have a reported colony forming efficiency of 0.83% in soft agarose.CELL PRODUCTS
To ensure your research is high quality, use authenticated ATCC cells. ATCC has the world’s largest and most extensive product catalog of human and animal cell lines for research purposes. The cell biology collection includes more than 4,000 continuous cell lines available by species, tissue/disease types, and signaling pathways. HUMAN CORONAVIRUS 229E Possibility of cross-reactions with other coronaviruses not thoroughly characterized at time of deposit (1973); does not cross-react with human myxoviruses. Ether sensitive. No hemolysis production. Unstable at 24 & 37C; relatively stable at 10C.Harvest 26-50 hrs post adsorption before CPE is visible. Does not agglutinate chicken, guinea pig, human O, sheep, mouse or rat RBC. Size 89MuT84 | ATCC
The original histological characteristics of the colon carcinoma were maintained throughout transplantation in nude mice. After 23 passages in athymic mice, the T84 cell line was established. These cells grow to confluence as monolayers and exhibit tight junctions and desmosomes between adjacent cells. They have receptors for many peptide hormones and neurotransmitters and maintain vectorialCHO-K1 | ATCC
Certificate of Analysis Download. To download a certificate of analysis for CHO-K1 ( CCL-61 ), enter the lot number exactly as it appears on your product label or packing slip. Lot number. Get Certificate of Analysis. Certificate of Analysis Request. The certificate of analysis for that lot of CHO-K1 ( CCL-61) is not currently available online.HELA | ATCC
Four typical HeLa marker chromosomes have been reported in the literature. HeLa Marker Chromosomes: One copy of Ml, one copy of M2, four-five copies of M3, and two copies of M4 as revealed by G-banding patterns. M1 is a rearranged long arm and centromere of chromosome 1MCF 10A | ATCC
The MCF 10A cell line is a non-tumorigenic epithelial cell line.The cells are positive for epithelial sialomucins, cytokeratins and milk fat globule antigen.They exhibit three dimensional growth in collagen, and form domes in confluent cultures.Thus far, the cells have shown no signs of terminal differentiation or senescence.The line is responsive to insulin, glucocorticoids, choleraNK-92® | ATCC
The cell line is dependent on the presence of recombinant Il-2 and a dose as low as 10 U/mL is sufficient to maintain proliferation; cells will die within 72 hours in the absence of IL-2. NK-92® cells have the following characteristics: surface marker positive for CD2, CD7, CD11a, CD28, CD45, CD54 and CD56 bright; surface marker negative for CD1, CD3, CD4, CD5, CD8, CD10, CD14, CD16, CD19MLE 12 | ATCC
Documentation. To download a certificate of analysis for MLE 12 ( CRL-2110 ), enter the lot number exactly as it appears on your product label or packing slip. The certificate of analysis for that lot of MLE 12 ( CRL-2110) is not currently available online. Complete this form to request this certificate of analysis.MDA-MB-231 | ATCC
The cells are somewhat spindle-shaped (long and thin) especially at subconfluence. Some cells in the culture usually remain rounded. There are always floaters. A photomicrograph of this cell line is available in the MDA-MB-231 (ATCC HTB-26) product description on the ATCCwebsite.
786-O
The cells display both microvilli and desmosomes, and can be grown in soft agar. (PubMed: 2835539) The cells produce a PTH like peptides that is identical to peptides produced by breast and lung tumors. The peptide has an N terminal sequence similar to PTH, ATCC: THE GLOBAL BIORESOURCE CENTER ATCC is a nonprofit organization that collects, stores, and distributes standard reference microorganisms, cell lines, and other materials for research and development. HUMAN CORONAVIRUS 229E Possibility of cross-reactions with other coronaviruses not thoroughly characterized at time of deposit (1973); does not cross-react with human myxoviruses. Ether sensitive. No hemolysis production. Unstable at 24 & 37C; relatively stable at 10C.Harvest 26-50 hrs post adsorption before CPE is visible. Does not agglutinate chicken, guinea pig, human O, sheep, mouse or rat RBC. Size 89MuMM.1S | ATCC
The parent cell line, MM.1, was established from peripheral blood of a multiple myeloma patient who had become resistant to steroid-based therapy. The closely related cell line, MM.1R ( ATCC CRL-2975) was also isolated from MM.1, but is resistant to dexamethasone.CCRF-CEM
Lot number. Get Certificate of Analysis. Certificate of Analysis Request. The certificate of analysis for that lot of CCRF-CEM ( CCL-119) is not currently available online. Complete this form to request this certificate of analysis. Account number. ATCC item number. Lot number. Email address.U-937 | ATCC
Studies since 1979 have shown that U-937 cells can be induced to terminal monocytic differentiation by supernatants from human mixed lymphocyte cultures, The cells are negative for immunoglobulin production and Epstein-Barr virus expression. The cells express the Fas antigen, and are sensitive to TNF and anti-Fas antibodies. In 1994, PCR and cytogenetic analyses showed that a number of stocksTHP-1 | ATCC
The lot specific information (number of cells and expected viability) from the Certificate of Analysis (COA) should be used to calculate the volume of medium necessary to start the THP-1 (ATCC TIB-202) cell culture at the recommended seeding density of 3 XCHO-K1 | ATCC
Certificate of Analysis Download. To download a certificate of analysis for CHO-K1 ( CCL-61 ), enter the lot number exactly as it appears on your product label or packing slip. Lot number. Get Certificate of Analysis. Certificate of Analysis Request. The certificate of analysis for that lot of CHO-K1 ( CCL-61) is not currently available online.MLE 12 | ATCC
Documentation. To download a certificate of analysis for MLE 12 ( CRL-2110 ), enter the lot number exactly as it appears on your product label or packing slip. The certificate of analysis for that lot of MLE 12 ( CRL-2110) is not currently available online. Complete this form to request this certificate of analysis.SUP-T1
The cells express multiple T lineage markers.The cells are E - rosette negative and beta 2 microglobulin positive.The cells are negative for Leu 5 (CD2), sheep erythrocyte receptor, OKT 9 (CD71; transferrin receptor), surface immunoglobulin (sIg -), HLA DR, CALLA (CD10) andTac (CD25).
MIA PACA-2 | ATCC
The MIA PaCa-2 cell line was established by A. Yunis, et al. in 1975 from tumor tissue of the pancreas obtained from a 65-year-old Caucasian male. Hybridomas have been made against the CSF-I produced by this line ATCC HB-8207 (F1A3-23) and ATCC HB-8208 (F18 AF1). ATCC: THE GLOBAL BIORESOURCE CENTER ATCC is a nonprofit organization that collects, stores, and distributes standard reference microorganisms, cell lines, and other materials for research and development. HUMAN CORONAVIRUS 229E Possibility of cross-reactions with other coronaviruses not thoroughly characterized at time of deposit (1973); does not cross-react with human myxoviruses. Ether sensitive. No hemolysis production. Unstable at 24 & 37C; relatively stable at 10C.Harvest 26-50 hrs post adsorption before CPE is visible. Does not agglutinate chicken, guinea pig, human O, sheep, mouse or rat RBC. Size 89MuMM.1S | ATCC
The parent cell line, MM.1, was established from peripheral blood of a multiple myeloma patient who had become resistant to steroid-based therapy. The closely related cell line, MM.1R ( ATCC CRL-2975) was also isolated from MM.1, but is resistant to dexamethasone.CCRF-CEM
Lot number. Get Certificate of Analysis. Certificate of Analysis Request. The certificate of analysis for that lot of CCRF-CEM ( CCL-119) is not currently available online. Complete this form to request this certificate of analysis. Account number. ATCC item number. Lot number. Email address.U-937 | ATCC
Studies since 1979 have shown that U-937 cells can be induced to terminal monocytic differentiation by supernatants from human mixed lymphocyte cultures, The cells are negative for immunoglobulin production and Epstein-Barr virus expression. The cells express the Fas antigen, and are sensitive to TNF and anti-Fas antibodies. In 1994, PCR and cytogenetic analyses showed that a number of stocksTHP-1 | ATCC
The lot specific information (number of cells and expected viability) from the Certificate of Analysis (COA) should be used to calculate the volume of medium necessary to start the THP-1 (ATCC TIB-202) cell culture at the recommended seeding density of 3 XCHO-K1 | ATCC
Certificate of Analysis Download. To download a certificate of analysis for CHO-K1 ( CCL-61 ), enter the lot number exactly as it appears on your product label or packing slip. Lot number. Get Certificate of Analysis. Certificate of Analysis Request. The certificate of analysis for that lot of CHO-K1 ( CCL-61) is not currently available online.MLE 12 | ATCC
Documentation. To download a certificate of analysis for MLE 12 ( CRL-2110 ), enter the lot number exactly as it appears on your product label or packing slip. The certificate of analysis for that lot of MLE 12 ( CRL-2110) is not currently available online. Complete this form to request this certificate of analysis.SUP-T1
The cells express multiple T lineage markers.The cells are E - rosette negative and beta 2 microglobulin positive.The cells are negative for Leu 5 (CD2), sheep erythrocyte receptor, OKT 9 (CD71; transferrin receptor), surface immunoglobulin (sIg -), HLA DR, CALLA (CD10) andTac (CD25).
MIA PACA-2 | ATCC
The MIA PaCa-2 cell line was established by A. Yunis, et al. in 1975 from tumor tissue of the pancreas obtained from a 65-year-old Caucasian male. Hybridomas have been made against the CSF-I produced by this line ATCC HB-8207 (F1A3-23) and ATCC HB-8208 (F18 AF1).CELL PRODUCTS
To ensure your research is high quality, use authenticated ATCC cells. ATCC has the world’s largest and most extensive product catalog of human and animal cell lines for research purposes. The cell biology collection includes more than 4,000 continuous cell lines available by species, tissue/disease types, and signaling pathways. HUMAN CORONAVIRUS 229E Possibility of cross-reactions with other coronaviruses not thoroughly characterized at time of deposit (1973); does not cross-react with human myxoviruses. Ether sensitive. No hemolysis production. Unstable at 24 & 37C; relatively stable at 10C.Harvest 26-50 hrs post adsorption before CPE is visible. Does not agglutinate chicken, guinea pig, human O, sheep, mouse or rat RBC.C2C12 | ATCC
The C2C12 cell line differentiates rapidly, forming contractile myotubes and producing characteristic muscle proteins.Treatment with bone morphogenic protein 2 (BMP-2) cause a shift in the differentiation pathway from myoblastic to osteoblastic.Tested and found negative for ectromelia virus (mousepox).MCF 10A | ATCC
The MCF 10A cell line is a non-tumorigenic epithelial cell line.The cells are positive for epithelial sialomucins, cytokeratins and milk fat globule antigen.They exhibit three dimensional growth in collagen, and form domes in confluent cultures.Thus far, the cells have shown no signs of terminal differentiation or senescence.The line is responsive to insulin, glucocorticoids, choleraHMC3 | ATCC
Resting HMC3 cells were strongly positive for the microglia/macrophage marker IBA1, positive for the endotoxin receptor CD14, but negative for the astrocyte marker GFAP. Markers of activated microglia, namely MHCII, CD68 and CD11b were negative in resting HMC3 cells, but upregulated after activation by IFN-gamma (10 ng/ml, 24 h).PANC-1 | ATCC
Remove medium, and rinse with 0.25% trypsin, 0.53mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.JURKAT, CLONE E6-1
To download a certificate of origin for Jurkat, Clone E6-1 ( TIB-152 ), enter the lot number exactly as it appears on your product label or packing slip. The certificate of origin for that lot of Jurkat, Clone E6-1 ( TIB-152) is not currently available online. Complete this form to request this certificate of origin.NCI-H460
To download a certificate of origin for NCI-H460 ( HTB-177 ), enter the lot number exactly as it appears on your product label or packing slip. The certificate of origin for that lot of NCI-H460 ( HTB-177) is not currently available online. Complete this form to request this certificate of origin. ISCOVE'S MODIFIED DULBECCO'S MEDIUM (IMDM) Formulation Contains 4 mM L-glutamine, 4500 mg/L glucose, and 1500 mg/L sodium bicarbonate NOTE: This reduced level of sodium bicarbonate (NaHCO 3, 1.5 g/L) is intended for use in a 5% CO 2 in air. Additional sodium bicarbonate may be required for use in incubators containing higher percentages of CO 2. CommentsVERO C1008
Documentation. To download a certificate of analysis for VERO C1008 ( CRL-1586 ), enter the lot number exactly as it appears on your product label or packing slip. The certificate of analysis for that lot of VERO C1008 ATCC: THE GLOBAL BIORESOURCE CENTER ATCC is a nonprofit organization that collects, stores, and distributes standard reference microorganisms, cell lines, and other materials for research and development.C2C12 | ATCC
The C2C12 cell line differentiates rapidly, forming contractile myotubes and producing characteristic muscle proteins.Treatment with bone morphogenic protein 2 (BMP-2) cause a shift in the differentiation pathway from myoblastic to osteoblastic.Tested and found negative for ectromelia virus (mousepox).U-937 | ATCC
Studies since 1979 have shown that U-937 cells can be induced to terminal monocytic differentiation by supernatants from human mixed lymphocyte cultures, The cells are negative for immunoglobulin production and Epstein-Barr virus expression. The cells express the Fas antigen, and are sensitive to TNF and anti-Fas antibodies. In 1994, PCR and cytogenetic analyses showed that a number of stocksCHO-K1 | ATCC
Certificate of Analysis Download. To download a certificate of analysis for CHO-K1 ( CCL-61 ), enter the lot number exactly as it appears on your product label or packing slip. Lot number. Get Certificate of Analysis. Certificate of Analysis Request. The certificate of analysis for that lot of CHO-K1 ( CCL-61) is not currently available online.HMC3 | ATCC
Resting HMC3 cells were strongly positive for the microglia/macrophage marker IBA1, positive for the endotoxin receptor CD14, but negative for the astrocyte marker GFAP. Markers of activated microglia, namely MHCII, CD68 and CD11b were negative in resting HMC3 cells, but upregulated after activation by IFN-gamma (10 ng/ml, 24 h).THP-1 | ATCC
The lot specific information (number of cells and expected viability) from the Certificate of Analysis (COA) should be used to calculate the volume of medium necessary to start the THP-1 (ATCC TIB-202) cell culture at the recommended seeding density of 3 XNCI-H460
To download a certificate of origin for NCI-H460 ( HTB-177 ), enter the lot number exactly as it appears on your product label or packing slip. The certificate of origin for that lot of NCI-H460 ( HTB-177) is not currently available online. Complete this form to request this certificate of origin.A-431 | ATCC
Documentation. To download a certificate of analysis for A-431 ( CRL-1555 ), enter the lot number exactly as it appears on your product label or packing slip. The certificate of analysis for that lot of A-431 ( CRL-1555) is not currently available online. Complete this form to request this certificate of analysis.786-O
The cells display both microvilli and desmosomes, and can be grown in soft agar. (PubMed: 2835539) The cells produce a PTH like peptides that is identical to peptides produced by breast and lung tumors. The peptide has an N terminal sequence similar to PTH,NK-92® | ATCC
The cell line is dependent on the presence of recombinant Il-2 and a dose as low as 10 U/mL is sufficient to maintain proliferation; cells will die within 72 hours in the absence of IL-2. NK-92® cells have the following characteristics: surface marker positive for CD2, CD7, CD11a, CD28, CD45, CD54 and CD56 bright; surface marker negative for CD1, CD3, CD4, CD5, CD8, CD10, CD14, CD16, CD19 ATCC: THE GLOBAL BIORESOURCE CENTER ATCC is a nonprofit organization that collects, stores, and distributes standard reference microorganisms, cell lines, and other materials for research and development.C2C12 | ATCC
The C2C12 cell line differentiates rapidly, forming contractile myotubes and producing characteristic muscle proteins.Treatment with bone morphogenic protein 2 (BMP-2) cause a shift in the differentiation pathway from myoblastic to osteoblastic.Tested and found negative for ectromelia virus (mousepox).U-937 | ATCC
Studies since 1979 have shown that U-937 cells can be induced to terminal monocytic differentiation by supernatants from human mixed lymphocyte cultures, The cells are negative for immunoglobulin production and Epstein-Barr virus expression. The cells express the Fas antigen, and are sensitive to TNF and anti-Fas antibodies. In 1994, PCR and cytogenetic analyses showed that a number of stocksCHO-K1 | ATCC
Certificate of Analysis Download. To download a certificate of analysis for CHO-K1 ( CCL-61 ), enter the lot number exactly as it appears on your product label or packing slip. Lot number. Get Certificate of Analysis. Certificate of Analysis Request. The certificate of analysis for that lot of CHO-K1 ( CCL-61) is not currently available online.HMC3 | ATCC
Resting HMC3 cells were strongly positive for the microglia/macrophage marker IBA1, positive for the endotoxin receptor CD14, but negative for the astrocyte marker GFAP. Markers of activated microglia, namely MHCII, CD68 and CD11b were negative in resting HMC3 cells, but upregulated after activation by IFN-gamma (10 ng/ml, 24 h).THP-1 | ATCC
The lot specific information (number of cells and expected viability) from the Certificate of Analysis (COA) should be used to calculate the volume of medium necessary to start the THP-1 (ATCC TIB-202) cell culture at the recommended seeding density of 3 XNCI-H460
To download a certificate of origin for NCI-H460 ( HTB-177 ), enter the lot number exactly as it appears on your product label or packing slip. The certificate of origin for that lot of NCI-H460 ( HTB-177) is not currently available online. Complete this form to request this certificate of origin.A-431 | ATCC
Documentation. To download a certificate of analysis for A-431 ( CRL-1555 ), enter the lot number exactly as it appears on your product label or packing slip. The certificate of analysis for that lot of A-431 ( CRL-1555) is not currently available online. Complete this form to request this certificate of analysis.786-O
The cells display both microvilli and desmosomes, and can be grown in soft agar. (PubMed: 2835539) The cells produce a PTH like peptides that is identical to peptides produced by breast and lung tumors. The peptide has an N terminal sequence similar to PTH,NK-92® | ATCC
The cell line is dependent on the presence of recombinant Il-2 and a dose as low as 10 U/mL is sufficient to maintain proliferation; cells will die within 72 hours in the absence of IL-2. NK-92® cells have the following characteristics: surface marker positive for CD2, CD7, CD11a, CD28, CD45, CD54 and CD56 bright; surface marker negative for CD1, CD3, CD4, CD5, CD8, CD10, CD14, CD16, CD19 ATCC: THE GLOBAL BIORESOURCE CENTER Place the ampule in a water bath at 37°C and agitate until the last bit of ice just thawed. Transfer the contents to a culture vessel with 10 volumes of appropriate medium. This ten-fold dilution will lower the concentration of the cryoprotective agent, such as DMSO, that could harm the cells.PANC-1 | ATCC
Remove medium, and rinse with 0.25% trypsin, 0.53mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.HELA | ATCC
Four typical HeLa marker chromosomes have been reported in the literature. HeLa Marker Chromosomes: One copy of Ml, one copy of M2, four-five copies of M3, and two copies of M4 as revealed by G-banding patterns. M1 is a rearranged long arm and centromere of chromosome 1MCF 10A | ATCC
The MCF 10A cell line is a non-tumorigenic epithelial cell line.The cells are positive for epithelial sialomucins, cytokeratins and milk fat globule antigen.They exhibit three dimensional growth in collagen, and form domes in confluent cultures.Thus far, the cells have shown no signs of terminal differentiation or senescence.The line is responsive to insulin, glucocorticoids, cholera HOME | ATCC GENOME PORTAL The ATCC Genome Portal is a repository of high-quality, authenticated microbial reference genomes.293
Although an earlier report suggested that the cells contained Adenovirus 5 DNA from both the right and left ends of the viral genome , it is now clear that only left end sequences are present.The cells express an unusual cell surface receptor for vitronectin composed of the integrin beta-1 subunit and the vitronectin receptor alpha-v subunit.The Ad5 insert was cloned andsequenced
MDA-MB-231 | ATCC
The cells are somewhat spindle-shaped (long and thin) especially at subconfluence. Some cells in the culture usually remain rounded. There are always floaters. A photomicrograph of this cell line is available in the MDA-MB-231 (ATCC HTB-26) product description on the ATCCwebsite.
HTERT RPE-1
The hTERT-immortalized retinal pigment epithelial cell line, hTERT RPE-1, was derived by transfecting the RPE-340 cell line with the pGRN145 hTERT-expressing plasmid ( ATCC MBA-141 ). Cells were cultured in medium containing hygromycin B until stable clones were selected.
ATCC.ORG
Object Moved This document may be found here ATCC: THE GLOBAL BIORESOURCE CENTER Object Moved This document may be found here ATCC: THE GLOBAL BIORESOURCE CENTER ATCC is a nonprofit organization that collects, stores, and distributes standard reference microorganisms, cell lines, and other materials for research and development.C2C12 | ATCC
The C2C12 cell line differentiates rapidly, forming contractile myotubes and producing characteristic muscle proteins.Treatment with bone morphogenic protein 2 (BMP-2) cause a shift in the differentiation pathway from myoblastic to osteoblastic.Tested and found negative for ectromelia virus (mousepox).U-937 | ATCC
Studies since 1979 have shown that U-937 cells can be induced to terminal monocytic differentiation by supernatants from human mixed lymphocyte cultures, The cells are negative for immunoglobulin production and Epstein-Barr virus expression. The cells express the Fas antigen, and are sensitive to TNF and anti-Fas antibodies. In 1994, PCR and cytogenetic analyses showed that a number of stocksCHO-K1 | ATCC
Certificate of Analysis Download. To download a certificate of analysis for CHO-K1 ( CCL-61 ), enter the lot number exactly as it appears on your product label or packing slip. Lot number. Get Certificate of Analysis. Certificate of Analysis Request. The certificate of analysis for that lot of CHO-K1 ( CCL-61) is not currently available online.HMC3 | ATCC
Resting HMC3 cells were strongly positive for the microglia/macrophage marker IBA1, positive for the endotoxin receptor CD14, but negative for the astrocyte marker GFAP. Markers of activated microglia, namely MHCII, CD68 and CD11b were negative in resting HMC3 cells, but upregulated after activation by IFN-gamma (10 ng/ml, 24 h).THP-1 | ATCC
The lot specific information (number of cells and expected viability) from the Certificate of Analysis (COA) should be used to calculate the volume of medium necessary to start the THP-1 (ATCC TIB-202) cell culture at the recommended seeding density of 3 XNCI-H460
To download a certificate of origin for NCI-H460 ( HTB-177 ), enter the lot number exactly as it appears on your product label or packing slip. The certificate of origin for that lot of NCI-H460 ( HTB-177) is not currently available online. Complete this form to request this certificate of origin.A-431 | ATCC
Documentation. To download a certificate of analysis for A-431 ( CRL-1555 ), enter the lot number exactly as it appears on your product label or packing slip. The certificate of analysis for that lot of A-431 ( CRL-1555) is not currently available online. Complete this form to request this certificate of analysis.NK-92® | ATCC
The cell line is dependent on the presence of recombinant Il-2 and a dose as low as 10 U/mL is sufficient to maintain proliferation; cells will die within 72 hours in the absence of IL-2. NK-92® cells have the following characteristics: surface marker positive for CD2, CD7, CD11a, CD28, CD45, CD54 and CD56 bright; surface marker negative for CD1, CD3, CD4, CD5, CD8, CD10, CD14, CD16, CD19786-O
The cells display both microvilli and desmosomes, and can be grown in soft agar. (PubMed: 2835539) The cells produce a PTH like peptides that is identical to peptides produced by breast and lung tumors. The peptide has an N terminal sequence similar to PTH, ATCC: THE GLOBAL BIORESOURCE CENTER ATCC is a nonprofit organization that collects, stores, and distributes standard reference microorganisms, cell lines, and other materials for research and development.C2C12 | ATCC
The C2C12 cell line differentiates rapidly, forming contractile myotubes and producing characteristic muscle proteins.Treatment with bone morphogenic protein 2 (BMP-2) cause a shift in the differentiation pathway from myoblastic to osteoblastic.Tested and found negative for ectromelia virus (mousepox).U-937 | ATCC
Studies since 1979 have shown that U-937 cells can be induced to terminal monocytic differentiation by supernatants from human mixed lymphocyte cultures, The cells are negative for immunoglobulin production and Epstein-Barr virus expression. The cells express the Fas antigen, and are sensitive to TNF and anti-Fas antibodies. In 1994, PCR and cytogenetic analyses showed that a number of stocksCHO-K1 | ATCC
Certificate of Analysis Download. To download a certificate of analysis for CHO-K1 ( CCL-61 ), enter the lot number exactly as it appears on your product label or packing slip. Lot number. Get Certificate of Analysis. Certificate of Analysis Request. The certificate of analysis for that lot of CHO-K1 ( CCL-61) is not currently available online.HMC3 | ATCC
Resting HMC3 cells were strongly positive for the microglia/macrophage marker IBA1, positive for the endotoxin receptor CD14, but negative for the astrocyte marker GFAP. Markers of activated microglia, namely MHCII, CD68 and CD11b were negative in resting HMC3 cells, but upregulated after activation by IFN-gamma (10 ng/ml, 24 h).THP-1 | ATCC
The lot specific information (number of cells and expected viability) from the Certificate of Analysis (COA) should be used to calculate the volume of medium necessary to start the THP-1 (ATCC TIB-202) cell culture at the recommended seeding density of 3 XNCI-H460
To download a certificate of origin for NCI-H460 ( HTB-177 ), enter the lot number exactly as it appears on your product label or packing slip. The certificate of origin for that lot of NCI-H460 ( HTB-177) is not currently available online. Complete this form to request this certificate of origin.A-431 | ATCC
Documentation. To download a certificate of analysis for A-431 ( CRL-1555 ), enter the lot number exactly as it appears on your product label or packing slip. The certificate of analysis for that lot of A-431 ( CRL-1555) is not currently available online. Complete this form to request this certificate of analysis.786-O
The cells display both microvilli and desmosomes, and can be grown in soft agar. (PubMed: 2835539) The cells produce a PTH like peptides that is identical to peptides produced by breast and lung tumors. The peptide has an N terminal sequence similar to PTH,NK-92® | ATCC
The cell line is dependent on the presence of recombinant Il-2 and a dose as low as 10 U/mL is sufficient to maintain proliferation; cells will die within 72 hours in the absence of IL-2. NK-92® cells have the following characteristics: surface marker positive for CD2, CD7, CD11a, CD28, CD45, CD54 and CD56 bright; surface marker negative for CD1, CD3, CD4, CD5, CD8, CD10, CD14, CD16, CD19PANC-1 | ATCC
Remove medium, and rinse with 0.25% trypsin, 0.53mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. ATCC: THE GLOBAL BIORESOURCE CENTER Place the ampule in a water bath at 37°C and agitate until the last bit of ice just thawed. Transfer the contents to a culture vessel with 10 volumes of appropriate medium. This ten-fold dilution will lower the concentration of the cryoprotective agent, such as DMSO, that could harm the cells.HELA | ATCC
Four typical HeLa marker chromosomes have been reported in the literature. HeLa Marker Chromosomes: One copy of Ml, one copy of M2, four-five copies of M3, and two copies of M4 as revealed by G-banding patterns. M1 is a rearranged long arm and centromere of chromosome 1MCF 10A | ATCC
The MCF 10A cell line is a non-tumorigenic epithelial cell line.The cells are positive for epithelial sialomucins, cytokeratins and milk fat globule antigen.They exhibit three dimensional growth in collagen, and form domes in confluent cultures.Thus far, the cells have shown no signs of terminal differentiation or senescence.The line is responsive to insulin, glucocorticoids, cholera293
Although an earlier report suggested that the cells contained Adenovirus 5 DNA from both the right and left ends of the viral genome , it is now clear that only left end sequences are present.The cells express an unusual cell surface receptor for vitronectin composed of the integrin beta-1 subunit and the vitronectin receptor alpha-v subunit.The Ad5 insert was cloned andsequenced
MDA-MB-231 | ATCC
The cells are somewhat spindle-shaped (long and thin) especially at subconfluence. Some cells in the culture usually remain rounded. There are always floaters. A photomicrograph of this cell line is available in the MDA-MB-231 (ATCC HTB-26) product description on the ATCCwebsite.
HTERT RPE-1
The hTERT-immortalized retinal pigment epithelial cell line, hTERT RPE-1, was derived by transfecting the RPE-340 cell line with the pGRN145 hTERT-expressing plasmid ( ATCC MBA-141 ). Cells were cultured in medium containing hygromycin B until stable clones were selected.
ATCC.ORG
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Object Moved This document may be found here ATCC: THE GLOBAL BIORESOURCE CENTER Object Moved This document may be found here ATCC: THE GLOBAL BIORESOURCE CENTER ATCC is a nonprofit organization that collects, stores, and distributes standard reference microorganisms, cell lines, and other materials for research and development.C2C12 | ATCC
The C2C12 cell line differentiates rapidly, forming contractile myotubes and producing characteristic muscle proteins.Treatment with bone morphogenic protein 2 (BMP-2) cause a shift in the differentiation pathway from myoblastic to osteoblastic.Tested and found negative for ectromelia virus (mousepox).U-937 | ATCC
Studies since 1979 have shown that U-937 cells can be induced to terminal monocytic differentiation by supernatants from human mixed lymphocyte cultures, The cells are negative for immunoglobulin production and Epstein-Barr virus expression. The cells express the Fas antigen, and are sensitive to TNF and anti-Fas antibodies. In 1994, PCR and cytogenetic analyses showed that a number of stocksCHO-K1 | ATCC
Certificate of Analysis Download. To download a certificate of analysis for CHO-K1 ( CCL-61 ), enter the lot number exactly as it appears on your product label or packing slip. Lot number. Get Certificate of Analysis. Certificate of Analysis Request. The certificate of analysis for that lot of CHO-K1 ( CCL-61) is not currently available online.HMC3 | ATCC
Resting HMC3 cells were strongly positive for the microglia/macrophage marker IBA1, positive for the endotoxin receptor CD14, but negative for the astrocyte marker GFAP. Markers of activated microglia, namely MHCII, CD68 and CD11b were negative in resting HMC3 cells, but upregulated after activation by IFN-gamma (10 ng/ml, 24 h).THP-1 | ATCC
The lot specific information (number of cells and expected viability) from the Certificate of Analysis (COA) should be used to calculate the volume of medium necessary to start the THP-1 (ATCC TIB-202) cell culture at the recommended seeding density of 3 XNCI-H460
To download a certificate of origin for NCI-H460 ( HTB-177 ), enter the lot number exactly as it appears on your product label or packing slip. The certificate of origin for that lot of NCI-H460 ( HTB-177) is not currently available online. Complete this form to request this certificate of origin.A-431 | ATCC
Documentation. To download a certificate of analysis for A-431 ( CRL-1555 ), enter the lot number exactly as it appears on your product label or packing slip. The certificate of analysis for that lot of A-431 ( CRL-1555) is not currently available online. Complete this form to request this certificate of analysis.NK-92® | ATCC
The cell line is dependent on the presence of recombinant Il-2 and a dose as low as 10 U/mL is sufficient to maintain proliferation; cells will die within 72 hours in the absence of IL-2. NK-92® cells have the following characteristics: surface marker positive for CD2, CD7, CD11a, CD28, CD45, CD54 and CD56 bright; surface marker negative for CD1, CD3, CD4, CD5, CD8, CD10, CD14, CD16, CD19786-O
The cells display both microvilli and desmosomes, and can be grown in soft agar. (PubMed: 2835539) The cells produce a PTH like peptides that is identical to peptides produced by breast and lung tumors. The peptide has an N terminal sequence similar to PTH, ATCC: THE GLOBAL BIORESOURCE CENTER ATCC is a nonprofit organization that collects, stores, and distributes standard reference microorganisms, cell lines, and other materials for research and development.C2C12 | ATCC
The C2C12 cell line differentiates rapidly, forming contractile myotubes and producing characteristic muscle proteins.Treatment with bone morphogenic protein 2 (BMP-2) cause a shift in the differentiation pathway from myoblastic to osteoblastic.Tested and found negative for ectromelia virus (mousepox).U-937 | ATCC
Studies since 1979 have shown that U-937 cells can be induced to terminal monocytic differentiation by supernatants from human mixed lymphocyte cultures, The cells are negative for immunoglobulin production and Epstein-Barr virus expression. The cells express the Fas antigen, and are sensitive to TNF and anti-Fas antibodies. In 1994, PCR and cytogenetic analyses showed that a number of stocksCHO-K1 | ATCC
Certificate of Analysis Download. To download a certificate of analysis for CHO-K1 ( CCL-61 ), enter the lot number exactly as it appears on your product label or packing slip. Lot number. Get Certificate of Analysis. Certificate of Analysis Request. The certificate of analysis for that lot of CHO-K1 ( CCL-61) is not currently available online.HMC3 | ATCC
Resting HMC3 cells were strongly positive for the microglia/macrophage marker IBA1, positive for the endotoxin receptor CD14, but negative for the astrocyte marker GFAP. Markers of activated microglia, namely MHCII, CD68 and CD11b were negative in resting HMC3 cells, but upregulated after activation by IFN-gamma (10 ng/ml, 24 h).THP-1 | ATCC
The lot specific information (number of cells and expected viability) from the Certificate of Analysis (COA) should be used to calculate the volume of medium necessary to start the THP-1 (ATCC TIB-202) cell culture at the recommended seeding density of 3 XNCI-H460
To download a certificate of origin for NCI-H460 ( HTB-177 ), enter the lot number exactly as it appears on your product label or packing slip. The certificate of origin for that lot of NCI-H460 ( HTB-177) is not currently available online. Complete this form to request this certificate of origin.A-431 | ATCC
Documentation. To download a certificate of analysis for A-431 ( CRL-1555 ), enter the lot number exactly as it appears on your product label or packing slip. The certificate of analysis for that lot of A-431 ( CRL-1555) is not currently available online. Complete this form to request this certificate of analysis.NK-92® | ATCC
The cell line is dependent on the presence of recombinant Il-2 and a dose as low as 10 U/mL is sufficient to maintain proliferation; cells will die within 72 hours in the absence of IL-2. NK-92® cells have the following characteristics: surface marker positive for CD2, CD7, CD11a, CD28, CD45, CD54 and CD56 bright; surface marker negative for CD1, CD3, CD4, CD5, CD8, CD10, CD14, CD16, CD19786-O
The cells display both microvilli and desmosomes, and can be grown in soft agar. (PubMed: 2835539) The cells produce a PTH like peptides that is identical to peptides produced by breast and lung tumors. The peptide has an N terminal sequence similar to PTH,PANC-1 | ATCC
Remove medium, and rinse with 0.25% trypsin, 0.53mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. ATCC: THE GLOBAL BIORESOURCE CENTER Place the ampule in a water bath at 37°C and agitate until the last bit of ice just thawed. Transfer the contents to a culture vessel with 10 volumes of appropriate medium. This ten-fold dilution will lower the concentration of the cryoprotective agent, such as DMSO, that could harm the cells.HELA | ATCC
Four typical HeLa marker chromosomes have been reported in the literature. HeLa Marker Chromosomes: One copy of Ml, one copy of M2, four-five copies of M3, and two copies of M4 as revealed by G-banding patterns. M1 is a rearranged long arm and centromere of chromosome 1MCF 10A | ATCC
The MCF 10A cell line is a non-tumorigenic epithelial cell line.The cells are positive for epithelial sialomucins, cytokeratins and milk fat globule antigen.They exhibit three dimensional growth in collagen, and form domes in confluent cultures.Thus far, the cells have shown no signs of terminal differentiation or senescence.The line is responsive to insulin, glucocorticoids, cholera293
Although an earlier report suggested that the cells contained Adenovirus 5 DNA from both the right and left ends of the viral genome , it is now clear that only left end sequences are present.The cells express an unusual cell surface receptor for vitronectin composed of the integrin beta-1 subunit and the vitronectin receptor alpha-v subunit.The Ad5 insert was cloned andsequenced
MDA-MB-231 | ATCC
The cells are somewhat spindle-shaped (long and thin) especially at subconfluence. Some cells in the culture usually remain rounded. There are always floaters. A photomicrograph of this cell line is available in the MDA-MB-231 (ATCC HTB-26) product description on the ATCCwebsite.
HTERT RPE-1
The hTERT-immortalized retinal pigment epithelial cell line, hTERT RPE-1, was derived by transfecting the RPE-340 cell line with the pGRN145 hTERT-expressing plasmid ( ATCC MBA-141 ). Cells were cultured in medium containing hygromycin B until stable clones were selected.
ATCC.ORG
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