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GENOME RESEARCHGR IN THE NEWSAUTHOR INFORMATIONE-MAIL ALERTS & RSS FEEDSFREE ONLINE ISSUEJOB OPPORTUNITIES Genome Research is an international, continuously published, peer-reviewed journal that features outstanding original research providing novel insights into the genome biology of all organisms, including significant advances in genomic medicine. The journal also provides high-quality reviews and perspectives written by respected leaders in the field and reports cutting-edge computational COLD SPRING HARBOR PERSPECTIVES IN BIOLOGY Cold Spring Harbor Perspectives in Biology — a new online publication spanning the complete spectrum of the molecular life sciences. Each issue includes reviews covering a wide variety of topics in molecular, cell, and developmental biology, genetics, neuroscience, immunology, cancer biology, and molecular pathology.GENES & DEVELOPMENT
Essential histone chaperones collaborate to regulate transcription and chromatin integrity. Meikin synergizes with shugoshin to protect cohesin Rec8 during meiosis I. MED1 is a lipogenesis coactivator required for postnatal adipose expansion. BAP1 constrains pervasive H2AK119ub1 to control the transcriptional potential of the genome.BIOFILMS - CSHL P
Biofilms are multicellular communities held together by a self-produced extracellular matrix. The mechanisms that different bacteria employ to form biofilms vary, frequently depending on environmental conditions and specific strain attributes. In this review, we emphasize four well-studied model systems to give an overview of how several THE RNASE PROTECTION ASSAY The RNase protection assay is a sensitive method for transcription start-site localization. It begins with an RNA probe that is uniformly labeled by incorporation of one NTP, usually UTP. The RNA probe is synthesized by bacteriophage RNA polymerase (SP6, T7, or T3), which initiates transcription from specific phage promoters SHORT INTRONIC REPEAT SEQUENCES FACILITATE CIRCULAR RNA Short intronic repeat sequences facilitate circular RNA production. Dongming Liang and. Jeremy E. Wilusz. + Author Affiliations. Department of Biochemistry and Biophysics, University of Pennsylvania Perelman School of Medicine, Philadelphia, Pennsylvania 19104, USA. Corresponding author: wilusz@mail.med.upenn.edu. KREBS-RINGER MODIFIED BUFFER (KRB) Krebs-Ringer Modified Buffer (KRB) Adjust the pH to 7.4. Most experiments are performed in buffer supplemented with 1 m m CaCl 2 . PARAFORMALDEHYDE IN PBS Paraformaldehyde in PBS. Paraformaldehyde, EM grade PBS (10X) NaOH (1 M) HCl (1 M) For a 4% paraformaldehyde solution, add 4 g of EM grade paraformaldehyde to 50 mL of H 2 O. Add 1 mL of 1 M NaOH and stir gently on a heating block at ~60°C until the paraformaldehyde is dissolved. Add 10 mL of 10X PBS and allow the mixture to cool to roomtemperature.
TERRIFIC BROTH (TB) MEDIUM Terrific Broth (TB) Medium. Add 900 mL of deionized water to 24 g of yeast extract, 20 g of tryptone, and 4 mL of glycerol. Shake or stir until the solutes have dissolved and sterilize by autoclaving for 20 min at 15 psi (1.05 kg/cm 2 ). Allow the solution to cool to ∼60°C and add 100 mL of sterile phosphate buffer. COLD SPRING HARBOR PROTOCOLSGENES & DEVELOPMENTALERTSSUBJECT CATEGORIESALTERNATE ROUTEHIGH-THROUGHPUT ANALYSISARCHIVE Cold Spring Harbor Protocols is now offering free full-access trial subscriptions . Cold Spring Harbor Molecular Case Studies Cold Spring Harbor Perspectives in Medicine Cold Spring Harbor Perspectives in Biology Genes & Development Cold Spring Harbor Symposia Genome Research Learning & Memory Life Science Alliance RNA Books and OtherMedia
GENOME RESEARCHGR IN THE NEWSAUTHOR INFORMATIONE-MAIL ALERTS & RSS FEEDSFREE ONLINE ISSUEJOB OPPORTUNITIES Genome Research is an international, continuously published, peer-reviewed journal that features outstanding original research providing novel insights into the genome biology of all organisms, including significant advances in genomic medicine. The journal also provides high-quality reviews and perspectives written by respected leaders in the field and reports cutting-edge computational COLD SPRING HARBOR PERSPECTIVES IN BIOLOGY Cold Spring Harbor Perspectives in Biology — a new online publication spanning the complete spectrum of the molecular life sciences. Each issue includes reviews covering a wide variety of topics in molecular, cell, and developmental biology, genetics, neuroscience, immunology, cancer biology, and molecular pathology.GENES & DEVELOPMENT
Essential histone chaperones collaborate to regulate transcription and chromatin integrity. Meikin synergizes with shugoshin to protect cohesin Rec8 during meiosis I. MED1 is a lipogenesis coactivator required for postnatal adipose expansion. BAP1 constrains pervasive H2AK119ub1 to control the transcriptional potential of the genome.BIOFILMS - CSHL P
Biofilms are multicellular communities held together by a self-produced extracellular matrix. The mechanisms that different bacteria employ to form biofilms vary, frequently depending on environmental conditions and specific strain attributes. In this review, we emphasize four well-studied model systems to give an overview of how several THE RNASE PROTECTION ASSAY The RNase protection assay is a sensitive method for transcription start-site localization. It begins with an RNA probe that is uniformly labeled by incorporation of one NTP, usually UTP. The RNA probe is synthesized by bacteriophage RNA polymerase (SP6, T7, or T3), which initiates transcription from specific phage promoters SHORT INTRONIC REPEAT SEQUENCES FACILITATE CIRCULAR RNA Short intronic repeat sequences facilitate circular RNA production. Dongming Liang and. Jeremy E. Wilusz. + Author Affiliations. Department of Biochemistry and Biophysics, University of Pennsylvania Perelman School of Medicine, Philadelphia, Pennsylvania 19104, USA. Corresponding author: wilusz@mail.med.upenn.edu. KREBS-RINGER MODIFIED BUFFER (KRB) Krebs-Ringer Modified Buffer (KRB) Adjust the pH to 7.4. Most experiments are performed in buffer supplemented with 1 m m CaCl 2 . PARAFORMALDEHYDE IN PBS Paraformaldehyde in PBS. Paraformaldehyde, EM grade PBS (10X) NaOH (1 M) HCl (1 M) For a 4% paraformaldehyde solution, add 4 g of EM grade paraformaldehyde to 50 mL of H 2 O. Add 1 mL of 1 M NaOH and stir gently on a heating block at ~60°C until the paraformaldehyde is dissolved. Add 10 mL of 10X PBS and allow the mixture to cool to roomtemperature.
TERRIFIC BROTH (TB) MEDIUM Terrific Broth (TB) Medium. Add 900 mL of deionized water to 24 g of yeast extract, 20 g of tryptone, and 4 mL of glycerol. Shake or stir until the solutes have dissolved and sterilize by autoclaving for 20 min at 15 psi (1.05 kg/cm 2 ). Allow the solution to cool to ∼60°C and add 100 mL of sterile phosphate buffer.GENOME RESEARCH
Genome Research is an international, continuously published, peer-reviewed journal that features outstanding original research providing novel insights into the genome biology of all organisms, including significant advances in genomic medicine. The journal also provides high-quality reviews and perspectives written by respected leaders in the field and reports cutting-edge computational POLYACRYLAMIDE GEL ELECTROPHORESIS Polyacrylamide gels have the following three major advantages over agarose gels: (1) Their resolving power is so great that they can separate molecules of DNA whose lengths differ by as little as 0.1% (i.e., 1 bp in 1000 bp). (2) They can accommodate much larger quantities of DNA than agarose gels. Up to 10 µg of DNA can beapplied to a single
GENES & DEVELOPMENT
Essential histone chaperones collaborate to regulate transcription and chromatin integrity. Meikin synergizes with shugoshin to protect cohesin Rec8 during meiosis I. MED1 is a lipogenesis coactivator required for postnatal adipose expansion. BAP1 constrains pervasive H2AK119ub1 to control the transcriptional potential of the genome. THE RNASE PROTECTION ASSAY The RNase protection assay is a sensitive method for transcription start-site localization. It begins with an RNA probe that is uniformly labeled by incorporation of one NTP, usually UTP. The RNA probe is synthesized by bacteriophage RNA polymerase (SP6, T7, or T3), which initiates transcription from specific phage promotersSIGN IN
A new type of review journal, featuring comprehensive collections of expert review articles on important topics in the molecular lifesciences
PARAFORMALDEHYDE IN PBS Paraformaldehyde in PBS. Paraformaldehyde, EM grade PBS (10X) NaOH (1 M) HCl (1 M) For a 4% paraformaldehyde solution, add 4 g of EM grade paraformaldehyde to 50 mL of H 2 O. Add 1 mL of 1 M NaOH and stir gently on a heating block at ~60°C until the paraformaldehyde is dissolved. Add 10 mL of 10X PBS and allow the mixture to cool to roomtemperature.
CONJUGATION OF ANTIBODIES TO HORSERADISH PEROXIDASE Abstract. Antibodies conjugated with horseradish peroxidase (HRP) are one of the most widely used bioreagents in the biological sciences. This protocol is a basic method for adding HRP to a thiolated antibody and can be adapted for use with different cross-linkers. MEGAPRIMER POLYMERASE CHAIN REACTION (PCR)-BASED MUTAGENESIS The megaprimer method is a simple and versatile approach that can be adopted to create a single mutation in a specific target region as well as to create site-specific insertions, deletions, and gene fusions. This method uses three oligonucleotide primers, two rounds of polymerase chain reaction (PCR), and a DNA template containing thegene to
MULTIPLE SCLEROSIS PATHOLOGY Multiple Sclerosis Pathology Hans Lassmann Center for Brain Research, Medical University of Vienna, A-1090 Wien, Austria Correspondence: hans.lassmann@meduniwien.ac.at CTAB EXTRACTION BUFFER CTAB extraction buffer. Prepare CTAB extraction buffer immediately before use; buffer is only good when freshly prepared. a Store 10% CTAB stock solution at 37°C to GENOME RESEARCHGR IN THE NEWSAUTHOR INFORMATIONE-MAIL ALERTS & RSS FEEDSFREE ONLINE ISSUEJOB OPPORTUNITIES Genome Research is an international, continuously published, peer-reviewed journal that features outstanding original research providing novel insights into the genome biology of all organisms, including significant advances in genomic medicine. The journal also provides high-quality reviews and perspectives written by respected leaders in the field and reports cutting-edge computational COLD SPRING HARBOR PERSPECTIVES IN BIOLOGYABOUTSUBJECT COLLECTIONSARCHIVESUBSCRIBEADVERTISEALERTS Cold Spring Harbor Perspectives in Biology — a new online publication spanning the complete spectrum of the molecular life sciences. Each issue includes reviews covering a wide variety of topics in molecular, cell, and developmental biology, genetics, neuroscience, immunology, cancer biology, and molecular pathology. COLD SPRING HARBOR PROTOCOLS For an alternate route to Cold Spring Harbor Protocols use this URL: http://intl-cshprotocols.cshlp.org http://intl-cshprotocols.cshlp.org [More THE RNASE PROTECTION ASSAY Abstract. The RNase protection assay is a sensitive method for transcription start-site localization. It begins with an RNA probe that is uniformly labeled by incorporation of one NTP, usually UTP.The RNA probe is synthesized by bacteriophage RNA polymerase (SP6, T7, or T3), which initiates transcription from specific phage promoters that have been engineered into a number ofBIOFILMS - CSHL P
Abstract. The ability to form biofilms is a universal attribute of bacteria. Biofilms are multicellular communities held together by a self-produced extracellular matrix.GENES & DEVELOPMENT
Preview contents, abstracts since 1987, with PDF available to subscribers. Includes article search, review collection, and online submission to Cold Spring Harbor Laboratory Press, Danvers, MA. SHORT INTRONIC REPEAT SEQUENCES FACILITATE CIRCULAR RNA Abstract. Recent deep sequencing studies have revealed thousands of circular noncoding RNAs generated from protein-coding genes. These RNAs are produced when the precursor messenger RNA (pre-mRNA) splicing machinery “backsplices” and covalently KREBS-RINGER MODIFIED BUFFER (KRB) doi:10.1101/pdb.rec076406 Cold Spring Harb Protoc 2014. 2014: pdb.rec076406- © 2014 Cold Spring Harbor Laboratory Press » FullText
ILLUMINA SEQUENCING LIBRARY PREPARATION FOR HIGHLY INTRODUCTION. The large amount of DNA sequence data generated by high-throughput sequencing technologies often allows multiple samples to be sequenced in parallel on a single sequencing run. TERRIFIC BROTH (TB) MEDIUM Reagent Quantity Final concentration; Yeast extract 24 g: 24 g/L: Tryptone 20 g: 20 g/L: Glycerol 4 mL: 4 mL/L: Phosphate buffer (0.17 m KH 2 PO 4, 0.72 m K 2 HPO 4) : 100 mL: 0.017 m KH 2 PO 4, 0.072 m K 2 HPO 4: Add 900 mL of deionized water to 24 g of yeast extract, 20 g of tryptone, and 4 mL of glycerol. GENOME RESEARCHGR IN THE NEWSAUTHOR INFORMATIONE-MAIL ALERTS & RSS FEEDSFREE ONLINE ISSUEJOB OPPORTUNITIES Genome Research is an international, continuously published, peer-reviewed journal that features outstanding original research providing novel insights into the genome biology of all organisms, including significant advances in genomic medicine. The journal also provides high-quality reviews and perspectives written by respected leaders in the field and reports cutting-edge computational COLD SPRING HARBOR PERSPECTIVES IN BIOLOGYABOUTSUBJECT COLLECTIONSARCHIVESUBSCRIBEADVERTISEALERTS Cold Spring Harbor Perspectives in Biology — a new online publication spanning the complete spectrum of the molecular life sciences. Each issue includes reviews covering a wide variety of topics in molecular, cell, and developmental biology, genetics, neuroscience, immunology, cancer biology, and molecular pathology. COLD SPRING HARBOR PROTOCOLS For an alternate route to Cold Spring Harbor Protocols use this URL: http://intl-cshprotocols.cshlp.org http://intl-cshprotocols.cshlp.org [More THE RNASE PROTECTION ASSAY Abstract. The RNase protection assay is a sensitive method for transcription start-site localization. It begins with an RNA probe that is uniformly labeled by incorporation of one NTP, usually UTP.The RNA probe is synthesized by bacteriophage RNA polymerase (SP6, T7, or T3), which initiates transcription from specific phage promoters that have been engineered into a number ofBIOFILMS - CSHL P
Abstract. The ability to form biofilms is a universal attribute of bacteria. Biofilms are multicellular communities held together by a self-produced extracellular matrix.GENES & DEVELOPMENT
Preview contents, abstracts since 1987, with PDF available to subscribers. Includes article search, review collection, and online submission to Cold Spring Harbor Laboratory Press, Danvers, MA. SHORT INTRONIC REPEAT SEQUENCES FACILITATE CIRCULAR RNA Abstract. Recent deep sequencing studies have revealed thousands of circular noncoding RNAs generated from protein-coding genes. These RNAs are produced when the precursor messenger RNA (pre-mRNA) splicing machinery “backsplices” and covalently KREBS-RINGER MODIFIED BUFFER (KRB) doi:10.1101/pdb.rec076406 Cold Spring Harb Protoc 2014. 2014: pdb.rec076406- © 2014 Cold Spring Harbor Laboratory Press » FullText
ILLUMINA SEQUENCING LIBRARY PREPARATION FOR HIGHLY INTRODUCTION. The large amount of DNA sequence data generated by high-throughput sequencing technologies often allows multiple samples to be sequenced in parallel on a single sequencing run. TERRIFIC BROTH (TB) MEDIUM Reagent Quantity Final concentration; Yeast extract 24 g: 24 g/L: Tryptone 20 g: 20 g/L: Glycerol 4 mL: 4 mL/L: Phosphate buffer (0.17 m KH 2 PO 4, 0.72 m K 2 HPO 4) : 100 mL: 0.017 m KH 2 PO 4, 0.072 m K 2 HPO 4: Add 900 mL of deionized water to 24 g of yeast extract, 20 g of tryptone, and 4 mL of glycerol. THE RNASE PROTECTION ASSAY Abstract. The RNase protection assay is a sensitive method for transcription start-site localization. It begins with an RNA probe that is uniformly labeled by incorporation of one NTP, usually UTP.The RNA probe is synthesized by bacteriophage RNA polymerase (SP6, T7, or T3), which initiates transcription from specific phage promoters that have been engineered into a number of POLYACRYLAMIDE GEL ELECTROPHORESIS Abstract. Cross-linked chains of polyacrylamide can be used as electrically neutral gels to separate double-stranded DNA fragments according to size and single-stranded DNAs according to CONJUGATION OF ANTIBODIES TO HORSERADISH PEROXIDASE Abstract. Antibodies conjugated with horseradish peroxidase (HRP) are one of the most widely used bioreagents in the biological sciences. This protocol is a basic method for adding HRP to a thiolated antibody and can be adapted for use with different cross-linkers. CTAB EXTRACTION BUFFER Reagent Amount to add (for 10 mL) Final concentration; Cetyltrimethyl ammonium bromide (CTAB) (10% in H 2 O) a: 3 mL: 3%: 5 M NaCl: 2.8 mL: 28%: 0.5 M EDTA (pH 8.0) 0.4 mL ORIGIN AND EVOLUTION OF THE RIBOSOME Abstract. The modern ribosome was largely formed at the time of the last common ancestor, LUCA. Hence its earliest origins likely lie inthe RNA world.
BONE MARROW-DERIVED MACROPHAGES (BMM): ISOLATION AND Figure 1. Flow chart of isolation and examples of applications for BMMs. Cells are isolated from bone marrow and cultured in vitro. BMMs are suitable for numerous applications including, but not limited to, the examples shown here.A MULTIPLE SCLEROSIS PATHOLOGY Multiple Sclerosis Pathology Hans Lassmann Center for Brain Research, Medical University of Vienna, A-1090 Wien, Austria Correspondence: hans.lassmann@meduniwien.ac.atAAV LYSIS BUFFER
Reagent Final concentration; Tris-HCl (pH 8.0) 50 m m: NaCl: 150 m m: Sterilize using a disposable 0.22-µm vacuum filtration system in a tissue-culture hood and store at room temperature. PARAFORMALDEHYDE IN PBS Paraformaldehyde in PBS. Paraformaldehyde, EM grade PBS (10X) NaOH (1 M) HCl (1 M) For a 4% paraformaldehyde solution, add 4 g of EM grade paraformaldehyde to 50 mL of H 2 O. Add 1 mL of 1 M NaOH and stir gently on a heating block at ~60°C until the paraformaldehyde is dissolved. Add 10 mL of 10X PBS and allow the mixture to cool to roomtemperature.
COOMASSIE BRILLIANT BLUE STAINING SOLUTION Coomassie Brilliant Blue staining solution. Dissolve 1 g of Coomassie Brilliant Blue (Bio-Rad) in 1 liter of the following solution:Methanol (50% )
GENOME RESEARCHGR IN THE NEWSAUTHOR INFORMATIONE-MAIL ALERTS & RSS FEEDSFREE ONLINE ISSUEJOB OPPORTUNITIES Genome Research is an international, continuously published, peer-reviewed journal that features outstanding original research providing novel insights into the genome biology of all organisms, including significant advances in genomic medicine. The journal also provides high-quality reviews and perspectives written by respected leaders in the field and reports cutting-edge computational COLD SPRING HARBOR PROTOCOLSGENES & DEVELOPMENTALERTSSUBJECT CATEGORIESALTERNATE ROUTEHIGH-THROUGHPUT ANALYSISARCHIVE Cold Spring Harbor Protocols is now offering free full-access trial subscriptions . Cold Spring Harbor Molecular Case Studies Cold Spring Harbor Perspectives in Medicine Cold Spring Harbor Perspectives in Biology Genes & Development Cold Spring Harbor Symposia Genome Research Learning & Memory Life Science Alliance RNA Books and OtherMedia
COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY Latest Complete Volume: 2019. Cold Spring Harbor Molecular Case Studies Cold Spring Harbor Perspectives in Medicine Cold Spring Harbor Perspectives in Biology Cold Spring Harbor Protocols Genes & Development Genome Research Learning & Memory Life Science AllianceRNA Books and
COLD SPRING HARBOR PERSPECTIVES IN BIOLOGY Cold Spring Harbor Perspectives in Biology — a new online publication spanning the complete spectrum of the molecular life sciences. Each issue includes reviews covering a wide variety of topics in molecular, cell, and developmental biology, genetics, neuroscience, immunology, cancer biology, and molecular pathology. MOLECULAR CASE STUDIES, A JOURNAL OF PRECISION MEDICINE Cold Spring Harbor Molecular Case Studies — an open-access journal in precision medicine. Articles present genomic and molecular analyses of individuals or cohorts alongside their clinical presentations and phenotypic information. The aim is to rapidly share insights into disease development and treatment gained by the application ofgenomics
COLD SPRING HARBOR PERSPECTIVES IN MEDICINE Cold Spring Harbor Perspectives in Medicine — a new online publication covering everything from the molecular and cellular bases of disease to translational medicine and emerging therapeutic strategies. Each issue includes reviews focused on different aspects of a variety of diseases. Contributions are written by leading expertsin each field
GENES & DEVELOPMENT
Essential histone chaperones collaborate to regulate transcription and chromatin integrity. Meikin synergizes with shugoshin to protect cohesin Rec8 during meiosis I. MED1 is a lipogenesis coactivator required for postnatal adipose expansion. BAP1 constrains pervasive H2AK119ub1 to control the transcriptional potential of the genome. DNA LOADING BUFFER (6X) DNA loading buffer (6X) 30% (v/v) glycerol. 0.25% (w/v) bromophenol blue. 0.25% (w/v) xylene cyanol FF. Store at 4°C. KREBS-RINGER MODIFIED BUFFER (KRB) Krebs-Ringer Modified Buffer (KRB) Adjust the pH to 7.4. Most experiments are performed in buffer supplemented with 1 m m CaCl 2 . CTAB EXTRACTION BUFFER CTAB extraction buffer. Prepare CTAB extraction buffer immediately before use; buffer is only good when freshly prepared. a Store 10% CTAB stock solution at 37°C to avoid precipitation. It GENOME RESEARCHGR IN THE NEWSAUTHOR INFORMATIONE-MAIL ALERTS & RSS FEEDSFREE ONLINE ISSUEJOB OPPORTUNITIES Genome Research is an international, continuously published, peer-reviewed journal that features outstanding original research providing novel insights into the genome biology of all organisms, including significant advances in genomic medicine. The journal also provides high-quality reviews and perspectives written by respected leaders in the field and reports cutting-edge computational COLD SPRING HARBOR PROTOCOLSGENES & DEVELOPMENTALERTSSUBJECT CATEGORIESALTERNATE ROUTEHIGH-THROUGHPUT ANALYSISARCHIVE Cold Spring Harbor Protocols is now offering free full-access trial subscriptions . Cold Spring Harbor Molecular Case Studies Cold Spring Harbor Perspectives in Medicine Cold Spring Harbor Perspectives in Biology Genes & Development Cold Spring Harbor Symposia Genome Research Learning & Memory Life Science Alliance RNA Books and OtherMedia
COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY Latest Complete Volume: 2019. Cold Spring Harbor Molecular Case Studies Cold Spring Harbor Perspectives in Medicine Cold Spring Harbor Perspectives in Biology Cold Spring Harbor Protocols Genes & Development Genome Research Learning & Memory Life Science AllianceRNA Books and
COLD SPRING HARBOR PERSPECTIVES IN BIOLOGY Cold Spring Harbor Perspectives in Biology — a new online publication spanning the complete spectrum of the molecular life sciences. Each issue includes reviews covering a wide variety of topics in molecular, cell, and developmental biology, genetics, neuroscience, immunology, cancer biology, and molecular pathology. MOLECULAR CASE STUDIES, A JOURNAL OF PRECISION MEDICINE Cold Spring Harbor Molecular Case Studies — an open-access journal in precision medicine. Articles present genomic and molecular analyses of individuals or cohorts alongside their clinical presentations and phenotypic information. The aim is to rapidly share insights into disease development and treatment gained by the application ofgenomics
COLD SPRING HARBOR PERSPECTIVES IN MEDICINE Cold Spring Harbor Perspectives in Medicine — a new online publication covering everything from the molecular and cellular bases of disease to translational medicine and emerging therapeutic strategies. Each issue includes reviews focused on different aspects of a variety of diseases. Contributions are written by leading expertsin each field
GENES & DEVELOPMENT
Essential histone chaperones collaborate to regulate transcription and chromatin integrity. Meikin synergizes with shugoshin to protect cohesin Rec8 during meiosis I. MED1 is a lipogenesis coactivator required for postnatal adipose expansion. BAP1 constrains pervasive H2AK119ub1 to control the transcriptional potential of the genome. DNA LOADING BUFFER (6X) DNA loading buffer (6X) 30% (v/v) glycerol. 0.25% (w/v) bromophenol blue. 0.25% (w/v) xylene cyanol FF. Store at 4°C. KREBS-RINGER MODIFIED BUFFER (KRB) Krebs-Ringer Modified Buffer (KRB) Adjust the pH to 7.4. Most experiments are performed in buffer supplemented with 1 m m CaCl 2 . CTAB EXTRACTION BUFFER CTAB extraction buffer. Prepare CTAB extraction buffer immediately before use; buffer is only good when freshly prepared. a Store 10% CTAB stock solution at 37°C to avoid precipitation. ItBIOFILMS - CSHL P
Biofilms are multicellular communities held together by a self-produced extracellular matrix. The mechanisms that different bacteria employ to form biofilms vary, frequently depending on environmental conditions and specific strain attributes. In this review, we emphasize four well-studied model systems to give an overview of how severalRNA
A monthly journal publishing high-quality, peer-reviewed research on all topics related to RNA and its metabolism in all organisms CONJUGATION OF ANTIBODIES TO HORSERADISH PEROXIDASE Abstract. Antibodies conjugated with horseradish peroxidase (HRP) are one of the most widely used bioreagents in the biological sciences. This protocol is a basic method for adding HRP to a thiolated antibody and can be adapted for use with different cross-linkers. SDS LOADING DYE (5X) SDS loading dye (5X) Bromophenol blue (0.02%) Tris-Cl (250 mM, pH 6.8) KREBS-RINGER MODIFIED BUFFER (KRB) Krebs-Ringer Modified Buffer (KRB) Adjust the pH to 7.4. Most experiments are performed in buffer supplemented with 1 m m CaCl 2 . PHOSPHATE-BUFFERED SALINE (PBS) Phosphate-buffered saline (PBS) PBS can be made as a 1× solution or as a 10× stock. To prepare 1 L of either 1× or 10× PBS, dissolve the reagents listed above in 800 mL of H 2 O. Adjust the pH to 7.4 (or 7.2, if required) with HCl, and then add H 2 O to 1 L. Dispense the solution into aliquots and sterilize them by autoclaving for 20 min at HEALTH BENEFITS OF EXERCISE The aim of this introduction is briefly to document facts that health benefits of physical activity predate its readers. In the 5th century BC, the ancient physician Hippocrates stated: “All parts of the body, if used in moderation and exercised in labors to which each is accustomed, become thereby healthy and well developed and age slowly; but if they are unused and left idle, they becomeEDTA - CSHL P
EDTA (ethylenediamenetetraacetic acid) NaOH. To prepare EDTA at 0.5 M (pH 8.0): Add 186.1 g of disodium EDTA•2H 2 O to 800 mL of H 2 O. Stir vigorously on a magnetic stirrer. Adjust the pH to 8.0 with NaOH (~20 g of NaOH pellets). Dispense into aliquots and sterilize by autoclaving. The disodium salt of EDTA will not go into solution until SODIUM ACETATE BUFFER (0.2 M, PH 5.0) Reagent. Quantity (for 2 L) Sodium acetate trihydrate. 54.43 g. Glacial acetic acid. 12 mL. H 2 O. 1988 mL. Combine the reagents and adjust the pH to 5.0 with 10 N NaOH. SOC MEDIUM FOR E. COLI SOC Medium for E. coli. Prepare a solution containing the first four reagents, sterilize at 121 °C, and then add sterile MgCl 2 andglucose.
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GUIDE TO THE HUMAN GENOMEBY STEWART SCHERER
> "The _Guide_ is not simply a textbook, a database, a review article, > or a reference book. By combining aspects of all of them, I hope it > is useful to students, faculty, and researchers." — Stewart> Scherer
Purchase the _Guide_ now . Presenting the genes of the human genome in their biological context, _Guide to the Human Genome_ is an extensive resource that provides easy access to information about human genes and their roles in specific processes. With numerous illustrations and tables, each of the nearly 300 sections of the _Guide_ describes genes involved in a specific pathway, process, or structure�from the molecular and cellular levels to developmental and physiological processes. This valuable resource is offered both online and in print. To order _The Guide_ for yourself or your institution, please CLICK HERE.
PLEASE CLICK ON THE BELOW TO: * View TABLE OF CONTENTS * View A SAMPLE CHAPTER * View a VIDEO ON HOW TO USE THE _GUIDE_ * PURCHASE THE _GUIDE_ NOW PLEASE NOTE THAT ERRATA ARE REPORTED IN THE BLOG.
FROM THE _PREFACE_
The availability of the human genome sequence has had an enormous impact on research but has not yet led to corresponding changes in how biology is presented to students. The origin of the Guide to the Human Genome is the idea that teaching biology requires easy access to genomic information and the accompanying information resources. To make this possible, I concluded that what was needed was a work organized like the textbooks used to teach biochemistry, cell biology, and related subjects, but one that provided direct connections to the extensive databases at NCBI. Simplifying access to sequences, search results, and other information would make possible much more realistic exercises for students in topics ranging from human genetics tosequence analysis.
A principal goal of the Guide is to provide a comprehensive framework to present all human genes. This initial version already provides quite extensive coverage of the reference human protein set. With the increasing application of genome-wide screens using various functional genomics approaches, researchers often encounter unfamiliar genes and pathways. This work can provide a consistent starting point for basic information about genes encountered in these types of experiments. In textbooks, much of the information is presented via model organisms where various processes have been most intensively studied. I have long felt that the extent of human genetic and biochemical data is not fully appreciated and that many of these topics can just as easily be presented using the human genes in those same processes. This approach should appeal to students planning careers in medicine and make the work of value in the basic science courses in medical schools. Generally, in a review, the goal is to try to say everything about something; here the goal is to try to say something about everything. To keep this initial effort manageable in size, even for the information that is included, it is not practical to present it in all of the places of the Guide where it might be relevant. Regular updates are planned for the Guide. Initially, the updates will be focused on individual sections where new information has become available and on handling errata. More comprehensive updates are planned, based primarily on when sufficient changes to the reference protein set have occurred. With the great diversity of topics in this work, feedback is essential for extending and improving it for the future. The homepage www.humangenomeguide.org has information about updates and contact information. Purchase the _Guide_ now . ------------------------- Home | Table of Contents| Search text
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_Guide to the Human Genome_ Copyright © 2010 by Stewart Scherer. All rights reserved.Details
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