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Detection
GERMAN COLLECTION OF MICROORGANISMS AND CELL CULTURES GMBH The search offers you access to our complete catalogue of DSMZ human and animal cell lines. New accessions are listed here; a complete list of cell lines from our collection is also available for download.. Mycoplasma PCR assay internal and positive control DNAs are available sending the order form via fax or e-mail to the specified number or address, respectively. GERMAN COLLECTION OF MICROORGANISMS AND CELL CULTURES GMBH The pragmatic species concept for Bacteria and Archaea is ultimately based on DNA-DNA hybridization (DDH), a method known to be tedious.The GGDC is a state-of-the-art in silico method for genome-to-genome comparison, thus reliably mimicking conventional DDH, except for its pitfalls.The method implemented in the GGDC was shown to outperform ANI, the “Average Nucleotide Identity” approach. GERMAN COLLECTION OF MICROORGANISMS AND CELL CULTURES GMBH Subculture: seed out at ca. 1.0 x 10 6 cells/ml; maintain at 0.5-1.5 x 10 6 cells/ml; split ratio of 1:3 to 1:4 every 3 days. Incubation: at 37 °C with 10% CO 2. Doubling time: about 48 hours. Harvest: maximum density of about 2.6 x 10 6 cells/ml. Storage: GERMAN COLLECTION OF MICROORGANISMS AND CELL CULTURES GMBH 80-90% RPMI 1640 + 10-20% h.i. FBS. Subculture: seed out at ca. 0.5 x 10 6 cells/ml (20% FBS and a 12- or 24-well plate could be of advantage); maintain at 0.2-1.0 x 10 6 cells/ml; split 1:2 to 1:3 every 3-4 days; note that after thawing cell viability may drop to about 40%, but cells will recover within a week. Incubation: at 37 °Cwith 5% CO 2.
GERMAN COLLECTION OF MICROORGANISMS AND CELL CULTURES GMBH seed out at about 1 x 10 6 cells/ml; optimal split ratio of about 1:2 to 1:3 every 2-3 days; maintain at 0.5-1.5 x 10 6 cells/ml. Incubation: at 37 °C with 5% CO 2. Doubling time: about 25-35 hours. Harvest: maximal density at about 1.5 x 10 6 cells/ml. Storage: frozen with 70% medium, 20% FBS, 10% DMSO. GERMAN COLLECTION OF MICROORGANISMS AND CELL Identification, Characterization & Authentication. Microbiological Analyses. Plant Virus Detection & Diagnostics. Deposit. MycoplasmaDetection
GERMAN COLLECTION OF MICROORGANISMS AND CELL CULTURES GMBH The search offers you access to our complete catalogue of DSMZ human and animal cell lines. New accessions are listed here; a complete list of cell lines from our collection is also available for download.. Mycoplasma PCR assay internal and positive control DNAs are available sending the order form via fax or e-mail to the specified number or address, respectively. GERMAN COLLECTION OF MICROORGANISMS AND CELL CULTURES GMBH The pragmatic species concept for Bacteria and Archaea is ultimately based on DNA-DNA hybridization (DDH), a method known to be tedious.The GGDC is a state-of-the-art in silico method for genome-to-genome comparison, thus reliably mimicking conventional DDH, except for its pitfalls.The method implemented in the GGDC was shown to outperform ANI, the “Average Nucleotide Identity” approach. GERMAN COLLECTION OF MICROORGANISMS AND CELL CULTURES GMBH Subculture: seed out at ca. 1.0 x 10 6 cells/ml; maintain at 0.5-1.5 x 10 6 cells/ml; split ratio of 1:3 to 1:4 every 3 days. Incubation: at 37 °C with 10% CO 2. Doubling time: about 48 hours. Harvest: maximum density of about 2.6 x 10 6 cells/ml. Storage: GERMAN COLLECTION OF MICROORGANISMS AND CELL CULTURES GMBH 80-90% RPMI 1640 + 10-20% h.i. FBS. Subculture: seed out at ca. 0.5 x 10 6 cells/ml (20% FBS and a 12- or 24-well plate could be of advantage); maintain at 0.2-1.0 x 10 6 cells/ml; split 1:2 to 1:3 every 3-4 days; note that after thawing cell viability may drop to about 40%, but cells will recover within a week. Incubation: at 37 °Cwith 5% CO 2.
GERMAN COLLECTION OF MICROORGANISMS AND CELL CULTURES GMBH seed out at about 1 x 10 6 cells/ml; optimal split ratio of about 1:2 to 1:3 every 2-3 days; maintain at 0.5-1.5 x 10 6 cells/ml. Incubation: at 37 °C with 5% CO 2. Doubling time: about 25-35 hours. Harvest: maximal density at about 1.5 x 10 6 cells/ml. Storage: frozen with 70% medium, 20% FBS, 10% DMSO. GERMAN COLLECTION OF MICROORGANISMS AND CELL The DSMZ is the most comprehensive biological resource center worldwide. Being one of the world's largest collections, the DSMZ currently comprises more than 73,700 items, including about 31,900 different bacterial and 6,600 fungal strains, 840 human and animal cell lines, 1,500 plant viruses and antisera, 700 bacteriophages and 19,000 different types of bacterial genomic DNA. GERMAN COLLECTION OF MICROORGANISMS AND CELL CULTURES GMBH Human and Animal Cell Lines. The DSMZ cell lines collection currently comprises more than 860 different immortalized cell cultures of primate, rodent, amphibian, fish, and insect origin isolated from numerous tissues as well as a number of hybridomas; continue tocatalogue.
GERMAN COLLECTION OF MICROORGANISMS AND CELL CULTURES GMBH The search offers you access to our complete catalogue of DSMZ human and animal cell lines. New accessions are listed here; a complete list of cell lines from our collection is also available for download.. Mycoplasma PCR assay internal and positive control DNAs are available sending the order form via fax or e-mail to the specified number or address, respectively. GERMAN COLLECTION OF MICROORGANISMS AND CELL CULTURES GMBH All deliveries to People's Republic of China and Republic of China will be carried out only against provision of an Entry Inspection Certificate issued by the local authorities and sent to DSMZ prior to sending the goods. For deliveries to China and Russia UN 3373 Biological Substance Category B actually is prohibited for all FedEx Express and regular postal services. GERMAN COLLECTION OF MICROORGANISMS AND CELL CULTURES GMBH Subculture: seed out at ca. 0.5 x 10 6 cells/ml; maintain at 0.1-1.0 x 10 6 cells/ml; split 1:3 to 1:5 every 3 days; viability may be low after thawing, but cells recover during the following 2-3 days; starting the culture with 20% FBS in 12- or 24-well-plates could be of advantage. Incubation: at GERMAN COLLECTION OF MICROORGANISMS AND CELL CULTURES GMBH seed out at about 1 x 10 6 cells/ml; optimal split ratio of about 1:2 to 1:3 every 2-3 days; maintain at 0.5-1.5 x 10 6 cells/ml. Incubation: at 37 °C with 5% CO 2. Doubling time: about 25-35 hours. Harvest: maximal density at about 1.5 x 10 6 cells/ml. Storage: frozen with 70% medium, 20% FBS, 10% DMSO. GERMAN COLLECTION OF MICROORGANISMS AND CELL CULTURES GMBH Host bacterium: Escherichia coli (Migula 1895) Castellani and Chalmers 1919. DSM-5695. We strongly recommend to use only DSM-5695 for propagation. Cultivation conditions: Medium 544 plus 2.0 mg of streptomycin per l, 37°C. Complete DSMZ Media List. Phage propagation: Supply, storage and propagation of phages. GERMAN COLLECTION OF MICROORGANISMS AND CELL CULTURES GMBH seed out at ca. 1-2 x 10 6 cells/80 cm 2; split confluent culture 1:4 to 1:6 every 3-5 days using trypsin/EDTA. Incubation: at 37 °C with 5% CO 2. Doubling time: ca. 40-48 hours. Harvest: cell harvest of ca. 10-15 x 10 6 cells/175 cm 2. Storage: frozen with 70% medium, 20% FBS,10% DMSO.
GERMAN COLLECTION OF MICROORGANISMS AND CELL CULTURES GMBH Cultivation conditions: Medium 1669 , anaerobic, 37°C. Dissolve the whole pellet in 500 µl of the oxygen-free medium and inoculate one Hungate tube. From here inoculate two tubes in addition in serial dilution (1:10). Pre-reduced Columbia blood agar plates can be inoculated from the grown liquid culture in addition. GERMAN COLLECTION OF MICROORGANISMS AND CELL CULTURES GMBH A. DSMZ Cell Culture Data: Morphology: polymorph adherent cells growing in monolayers and in small clumps; image; image. Medium: 80% RPMI 1640 + 20% h.i. FBS. Subculture: seed out at ca. 1 x 10 6 cells/25 cm 2 flask; split confluent culture 1:2 to 1:3 every 4-6 days using trypsin/EDTA (cells are only slightly adherent and can also bedetached
GERMAN COLLECTION OF MICROORGANISMS AND CELL Identification, Characterization & Authentication. Microbiological Analyses. Plant Virus Detection & Diagnostics. Deposit. MycoplasmaDetection
GERMAN COLLECTION OF MICROORGANISMS AND CELL CULTURES GMBH The pragmatic species concept for Bacteria and Archaea is ultimately based on DNA-DNA hybridization (DDH), a method known to be tedious.The GGDC is a state-of-the-art in silico method for genome-to-genome comparison, thus reliably mimicking conventional DDH, except for its pitfalls.The method implemented in the GGDC was shown to outperform ANI, the “Average Nucleotide Identity” approach. GERMAN COLLECTION OF MICROORGANISMS AND CELL CULTURES GMBH Subculture: seed out at ca. 0.5 x 10 6 cells/ml; maintain at 0.1-1.0 x 10 6 cells/ml; split 1:3 to 1:5 every 3 days; viability may be low after thawing, but cells recover during the following 2-3 days; starting the culture with 20% FBS in 12- or 24-well-plates could be of advantage. Incubation: at GERMAN COLLECTION OF MICROORGANISMS AND CELL CULTURES GMBH Subculture: seed out at about 2-3 x 10 6 cells/25 cm 2; split confluent culture 1:5 to 1:6 every 2-3 days (with using trypsin/EDTA, cells can be detached by tapping the flask) Incubation: at 37 °C with 10% CO 2. Doubling time: about 24-30 hours. Harvest: cell harvest of about 20 x 10 6 cells/80 cm 2. Storage: GERMAN COLLECTION OF MICROORGANISMS AND CELL CULTURES GMBH Digital Sequence Information. With the publication of the IPBES Report on May 6, 2019, awareness has grown that the human-induced loss of biological diversity and the extinction of species. This report and other science-based assessments form the basis for the negotiations of the post-2020 Global Biodiversity Framework, which will enable a GERMAN COLLECTION OF MICROORGANISMS AND CELL CULTURES GMBH Type strain. Taxonomy/description (1300, 1423). Sequence accession no. 16S rRNA gene: X96954, complete genome: CP002987. Produces hydrogenase (1425). Mixotrophic growth (1425). Tetrahydrofolate enzyme levels (1424). (Medium 135, 30°C, anaerobic). Freight and handling charges will be added. See price list. GERMAN COLLECTION OF MICROORGANISMS AND CELL CULTURES GMBH Utilizes 1-butanol (4303), 2-propanol (4303), methanol (4303). Application in exhaust gas biofilters (4303). (Medium 1, 30°C or Medium 830, 30°C). For detailed information: Literature: 742, 1300, 4303, 4602, 6182, 7293. Risk group: 1 (classification according to German TRBA) Supplied as: GERMAN COLLECTION OF MICROORGANISMS AND CELL Identification, Characterization & Authentication. Microbiological Analyses. Plant Virus Detection & Diagnostics. Deposit. MycoplasmaDetection
GERMAN COLLECTION OF MICROORGANISMS AND CELL CULTURES GMBH The pragmatic species concept for Bacteria and Archaea is ultimately based on DNA-DNA hybridization (DDH), a method known to be tedious.The GGDC is a state-of-the-art in silico method for genome-to-genome comparison, thus reliably mimicking conventional DDH, except for its pitfalls.The method implemented in the GGDC was shown to outperform ANI, the “Average Nucleotide Identity” approach. GERMAN COLLECTION OF MICROORGANISMS AND CELL CULTURES GMBH Subculture: seed out at ca. 0.5 x 10 6 cells/ml; maintain at 0.1-1.0 x 10 6 cells/ml; split 1:3 to 1:5 every 3 days; viability may be low after thawing, but cells recover during the following 2-3 days; starting the culture with 20% FBS in 12- or 24-well-plates could be of advantage. Incubation: at GERMAN COLLECTION OF MICROORGANISMS AND CELL CULTURES GMBH Subculture: seed out at about 2-3 x 10 6 cells/25 cm 2; split confluent culture 1:5 to 1:6 every 2-3 days (with using trypsin/EDTA, cells can be detached by tapping the flask) Incubation: at 37 °C with 10% CO 2. Doubling time: about 24-30 hours. Harvest: cell harvest of about 20 x 10 6 cells/80 cm 2. Storage: GERMAN COLLECTION OF MICROORGANISMS AND CELL CULTURES GMBH Digital Sequence Information. With the publication of the IPBES Report on May 6, 2019, awareness has grown that the human-induced loss of biological diversity and the extinction of species. This report and other science-based assessments form the basis for the negotiations of the post-2020 Global Biodiversity Framework, which will enable a GERMAN COLLECTION OF MICROORGANISMS AND CELL CULTURES GMBH Type strain. Taxonomy/description (1300, 1423). Sequence accession no. 16S rRNA gene: X96954, complete genome: CP002987. Produces hydrogenase (1425). Mixotrophic growth (1425). Tetrahydrofolate enzyme levels (1424). (Medium 135, 30°C, anaerobic). Freight and handling charges will be added. See price list. GERMAN COLLECTION OF MICROORGANISMS AND CELL CULTURES GMBH Utilizes 1-butanol (4303), 2-propanol (4303), methanol (4303). Application in exhaust gas biofilters (4303). (Medium 1, 30°C or Medium 830, 30°C). For detailed information: Literature: 742, 1300, 4303, 4602, 6182, 7293. Risk group: 1 (classification according to German TRBA) Supplied as: GERMAN COLLECTION OF MICROORGANISMS AND CELL The DSMZ is the most comprehensive biological resource center worldwide. Being one of the world's largest collections, the DSMZ currently comprises more than 73,700 items, including about 31,900 different bacterial and 6,600 fungal strains, 840 human and animal cell lines, 1,500 plant viruses and antisera, 700 bacteriophages and 19,000 different types of bacterial genomic DNA. GERMAN COLLECTION OF MICROORGANISMS AND CELL CULTURES GMBH Human and Animal Cell Lines. The DSMZ cell lines collection currently comprises more than 860 different immortalized cell cultures of primate, rodent, amphibian, fish, and insect origin isolated from numerous tissues as well as a number of hybridomas; continue tocatalogue.
GERMAN COLLECTION OF MICROORGANISMS AND CELL CULTURES GMBH Subculture: seed out at about 2-3 x 10 6 cells/25 cm 2; split confluent culture 1:5 to 1:6 every 2-3 days (with using trypsin/EDTA, cells can be detached by tapping the flask) Incubation: at 37 °C with 10% CO 2. Doubling time: about 24-30 hours. Harvest: cell harvest of about 20 x 10 6 cells/80 cm 2. Storage: GERMAN COLLECTION OF MICROORGANISMS AND CELL CULTURES GMBH seed out at about 1 x 10 6 cells/ml; optimal split ratio of about 1:2 to 1:3 every 2-3 days; maintain at 0.5-1.5 x 10 6 cells/ml. Incubation: at 37 °C with 5% CO 2. Doubling time: about 25-35 hours. Harvest: maximal density at about 1.5 x 10 6 cells/ml. Storage: frozen with 70% medium, 20% FBS, 10% DMSO. GERMAN COLLECTION OF MICROORGANISMS AND CELL CULTURES GMBH Incubation: at 37 °C with 5% CO 2. Doubling time: ca. 40-50 hours. Harvest: maximum density of about 0.8 x 10 6 cells/ml. Storage: frozen with 70% medium, 20% FBS, 10% DMSO. DSMZ Scientific Data: GERMAN COLLECTION OF MICROORGANISMS AND CELL CULTURES GMBH Subculture: seed out at ca. 1.0 x 10 6 cells/ml; maintain at 0.5-1.5 x 10 6 cells/ml; split ratio of 1:3 to 1:4 every 3 days. Incubation: at 37 °C with 10% CO 2. Doubling time: about 48 hours. Harvest: maximum density of about 2.6 x 10 6 cells/ml. Storage: GERMAN COLLECTION OF MICROORGANISMS AND CELL CULTURES GMBH Type strain. Taxonomy/description (1300, 1423). Sequence accession no. 16S rRNA gene: X96954, complete genome: CP002987. Produces hydrogenase (1425). Mixotrophic growth (1425). Tetrahydrofolate enzyme levels (1424). (Medium 135, 30°C, anaerobic). Freight and handling charges will be added. See price list. GERMAN COLLECTION OF MICROORGANISMS AND CELL CULTURES GMBH seed out at ca. 1-2 x 10 6 cells/80 cm 2 flask; split confluent culture 1:2 to 1:5 every 3-5 days using trypsin/EDTA. Incubation: at 37 °C with 10% CO 2. Doubling time: ca. 40-50 hours. Harvest: cell harvest of ca. 8 x 10 6 cells/80 cm 2. Storage: frozen with 70% medium, 20% FBS, 10% DMSO. GERMAN COLLECTION OF MICROORGANISMS AND CELL CULTURES GMBH levels of oxygen in the atmosphere. For instance T Clostridium intestinale DSM 6191 can grow well on the GERMAN COLLECTION OF MICROORGANISMS AND CELL CULTURES GMBH Leibniz Institute DSMZ-German Collection of Microorganisms and CellCultures GmbH
GERMAN COLLECTION OF MICROORGANISMS AND CELL Identification, Characterization & Authentication. Microbiological Analyses. Plant Virus Detection & Diagnostics. Deposit. MycoplasmaDetection
GERMAN COLLECTION OF MICROORGANISMS AND CELL CULTURES GMBH Human and Animal Cell Lines. The DSMZ cell lines collection currently comprises more than 860 different immortalized cell cultures of primate, rodent, amphibian, fish, and insect origin isolated from numerous tissues as well as a number of hybridomas; continue tocatalogue.
GERMAN COLLECTION OF MICROORGANISMS AND CELL CULTURES GMBH The pragmatic species concept for Bacteria and Archaea is ultimately based on DNA-DNA hybridization (DDH), a method known to be tedious.The GGDC is a state-of-the-art in silico method for genome-to-genome comparison, thus reliably mimicking conventional DDH, except for its pitfalls.The method implemented in the GGDC was shown to outperform ANI, the “Average Nucleotide Identity” approach. GERMAN COLLECTION OF MICROORGANISMS AND CELL CULTURES GMBH Subculture: seed out at ca. 0.5 x 10 6 cells/ml; maintain at 0.1-1.0 x 10 6 cells/ml; split 1:3 to 1:5 every 3 days; viability may be low after thawing, but cells recover during the following 2-3 days; starting the culture with 20% FBS in 12- or 24-well-plates could be of advantage. Incubation: at GERMAN COLLECTION OF MICROORGANISMS AND CELL CULTURES GMBH Incubation: at 37 °C with 5% CO 2. Doubling time: ca. 40-50 hours. Harvest: maximum density of about 0.8 x 10 6 cells/ml. Storage: frozen with 70% medium, 20% FBS, 10% DMSO. DSMZ Scientific Data: GERMAN COLLECTION OF MICROORGANISMS AND CELL CULTURES GMBH Subculture: seed out at ca. 1.0 x 10 6 cells/ml; maintain at 0.5-1.5 x 10 6 cells/ml; split ratio of 1:3 to 1:4 every 3 days. Incubation: at 37 °C with 10% CO 2. Doubling time: about 48 hours. Harvest: maximum density of about 2.6 x 10 6 cells/ml. Storage: GERMAN COLLECTION OF MICROORGANISMS AND CELL Identification, Characterization & Authentication. Microbiological Analyses. Plant Virus Detection & Diagnostics. Deposit. MycoplasmaDetection
GERMAN COLLECTION OF MICROORGANISMS AND CELL CULTURES GMBH Human and Animal Cell Lines. The DSMZ cell lines collection currently comprises more than 860 different immortalized cell cultures of primate, rodent, amphibian, fish, and insect origin isolated from numerous tissues as well as a number of hybridomas; continue tocatalogue.
GERMAN COLLECTION OF MICROORGANISMS AND CELL CULTURES GMBH The pragmatic species concept for Bacteria and Archaea is ultimately based on DNA-DNA hybridization (DDH), a method known to be tedious.The GGDC is a state-of-the-art in silico method for genome-to-genome comparison, thus reliably mimicking conventional DDH, except for its pitfalls.The method implemented in the GGDC was shown to outperform ANI, the “Average Nucleotide Identity” approach. GERMAN COLLECTION OF MICROORGANISMS AND CELL CULTURES GMBH Subculture: seed out at ca. 0.5 x 10 6 cells/ml; maintain at 0.1-1.0 x 10 6 cells/ml; split 1:3 to 1:5 every 3 days; viability may be low after thawing, but cells recover during the following 2-3 days; starting the culture with 20% FBS in 12- or 24-well-plates could be of advantage. Incubation: at GERMAN COLLECTION OF MICROORGANISMS AND CELL CULTURES GMBH Incubation: at 37 °C with 5% CO 2. Doubling time: ca. 40-50 hours. Harvest: maximum density of about 0.8 x 10 6 cells/ml. Storage: frozen with 70% medium, 20% FBS, 10% DMSO. DSMZ Scientific Data: GERMAN COLLECTION OF MICROORGANISMS AND CELL CULTURES GMBH Subculture: seed out at ca. 1.0 x 10 6 cells/ml; maintain at 0.5-1.5 x 10 6 cells/ml; split ratio of 1:3 to 1:4 every 3 days. Incubation: at 37 °C with 10% CO 2. Doubling time: about 48 hours. Harvest: maximum density of about 2.6 x 10 6 cells/ml. Storage: GERMAN COLLECTION OF MICROORGANISMS AND CELL CULTURES GMBH seed out at about 1 x 10 6 cells/ml; optimal split ratio of about 1:2 to 1:3 every 2-3 days; maintain at 0.5-1.5 x 10 6 cells/ml. Incubation: at 37 °C with 5% CO 2. Doubling time: about 25-35 hours. Harvest: maximal density at about 1.5 x 10 6 cells/ml. Storage: frozen with 70% medium, 20% FBS, 10% DMSO. GERMAN COLLECTION OF MICROORGANISMS AND CELL CULTURES GMBH 80-90% RPMI 1640 + 10-20% h.i. FBS. Subculture: seed out at ca. 0.5 x 10 6 cells/ml (20% FBS and a 12- or 24-well plate could be of advantage); maintain at 0.2-1.0 x 10 6 cells/ml; split 1:2 to 1:3 every 3-4 days; note that after thawing cell viability may drop to about 40%, but cells will recover within a week. Incubation: at 37 °Cwith 5% CO 2.
GERMAN COLLECTION OF MICROORGANISMS AND CELL CULTURES GMBH Subculture: seed out at about 2-3 x 10 6 cells/25 cm 2; split confluent culture 1:5 to 1:6 every 2-3 days (with using trypsin/EDTA, cells can be detached by tapping the flask) Incubation: at 37 °C with 10% CO 2. Doubling time: about 24-30 hours. Harvest: cell harvest of about 20 x 10 6 cells/80 cm 2. Storage: GERMAN COLLECTION OF MICROORGANISMS AND CELL CULTURES GMBH Subculture: seed out at ca. 0.5 x 10 6 cells/ml; maintain at 0.1-1.0 x 10 6 cells/ml; split 1:3 to 1:5 every 3 days; viability may be low after thawing, but cells recover during the following 2-3 days; starting the culture with 20% FBS in 12- or 24-well-plates could be of advantage. Incubation: at GERMAN COLLECTION OF MICROORGANISMS AND CELL CULTURES GMBH Type strain. Taxonomy/description (1300, 1423). Sequence accession no. 16S rRNA gene: X96954, complete genome: CP002987. Produces hydrogenase (1425). Mixotrophic growth (1425). Tetrahydrofolate enzyme levels (1424). (Medium 135, 30°C, anaerobic). Freight and handling charges will be added. See price list. GERMAN COLLECTION OF MICROORGANISMS AND CELL CULTURES GMBH seed out at ca. 1-2 x 10 6 cells/80 cm 2 flask; split confluent culture 1:2 to 1:5 every 3-5 days using trypsin/EDTA. Incubation: at 37 °C with 10% CO 2. Doubling time: ca. 40-50 hours. Harvest: cell harvest of ca. 8 x 10 6 cells/80 cm 2. Storage: frozen with 70% medium, 20% FBS, 10% DMSO. GERMAN COLLECTION OF MICROORGANISMS AND CELL CULTURES GMBH seed out at ca. 0.3-0.5 x 10 6 cells/ml in flasks; maintain at ca. 0.5-3.0 x 10 6 cells/ml; split culture 1:3 every 2 days. Incubation: at 37 °C with 5% CO 2. Doubling time: ca. 20-24 hours. Harvest: maximum density at ca. 3.5 x 10 6 cells/ml. Storage: frozen with 70% medium, 20% FBS, 10% DMSO. 104. PYG MEDIUM (MODIFIED) 2020 DSMZ GmbH - All rights reserved 104. PYG MEDIUM (modified) Trypticase peptone 5.00 g Peptone 5.00 g Yeast extract 10.00 g Beefextract 5.00 g
GERMAN COLLECTION OF MICROORGANISMS AND CELL CULTURES GMBH DSMZ - Collection. The Leibniz Institute DSMZ is the world's most diverse collection of biological resources (bacteria, archaea, protists, yeasts, fungi, bacteriophages, plant viruses, genomic bacterial DNA as well as human and animal cell lines). Microorganisms and cell cultures are collected, investigated and archived at theDSMZ.
LPSN - LIST OF PROKARYOTIC NAMES WITH STANDING IN NOMENCLATURE LPSN - List of Prokaryotic names with Standing in Nomenclature Founded in 1997 by Jean P. Euzéby. About The List of Prokaryotic names with Standing in Nomenclature provides comprehensive information on the nomenclature of prokaryotes and much more.Navigating LPSN is easy. LPSN is a free to use service founded by Jean P. Euzéby in 1997 and later on maintained by Aidan C. Parte.DSMZ
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Search Menu Search term Search: WELCOME TO THE LEIBNIZ INSTITUTE DSMZ GERMAN COLLECTION OF MICROORGANISMS AND CELL CULTURES GMBHRESEARCH
The research focus of the almost 100 scientists at the DSMZ are the various aspects of biodiversity in prokaryotes & eukaryotes.Research groups
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NEWS
06.08.2019
BUNDESPOLITIKER DER LINKEN BESUCHT DIE MIKROBENMETROPOLE DSMZ MdB Victor Perli besuchte die weltweit vielfältigste Sammlung von Mikroorganismen und Zellkulturen in BraunschweigRead more
BIORESOURCES
BACTERIA
> 31,000 strains
ARCHAEA
> 680 strains
PLANT VIRUSES
> 760 viruses
HUMAN & ANIMAL CELL LINES> 840 cell lines
PLASMIDS
> 250 plasmids
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> 6,500 strains
BACTERIOPHAGES
> 670 phages
PROTISTS
> 45 strains
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Plant Viruses
IN THE FOCUS ...
NEW PARSLEY VIRUS DISCOVERED BY BRAUNSCHWEIG RESEARCHERS Plant virologists Dr. Björn Krenz and Dr. Stephan Winter from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures in Braunschweig and his colleagues have identified a new parsley virus and published in the peer-review journal Archives ofVirology. More ...
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THE BACTERIAL DIVERSITY METADATABASE Our metadatabase of organism-linked information covering the multifarious aspects of bacterial and archaeal biodiversity.TYGS
TYPE (STRAIN) GENOME SERVER TYGS offers a truly genome-based classification and identification of prokaryotic strains without overestimating phylogeneticconfidence.
PNU
NOMENCLATURE UP-TO-DATE Nomenclature up-to-date is a compilation of all names of Bacteria & Archaea which have been validly published according to the Bacteriological Code. SHORT TANDEM REPEAT (STR) PROFILE SEARCH ANALYSIS OF SHORT TANDEM REPEATS Since STR microsatellites are stable as well as highly polymorphic in human populations, STR profiling is shown to be ideal for authentication of human cell line samples. DO YOU NEED SUPPORT? CONTACT THE CUSTOMER SUPPORT .DSMZ
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