Are you over 18 and want to see adult content?
More Annotations
A complete backup of decisiondeskhq.com
Are you over 18 and want to see adult content?
A complete backup of dossierjournal.com
Are you over 18 and want to see adult content?
A complete backup of batangastoday.com
Are you over 18 and want to see adult content?
Favourite Annotations
A complete backup of mandieselturbo.com
Are you over 18 and want to see adult content?
A complete backup of importexport.com.pk
Are you over 18 and want to see adult content?
A complete backup of mosurbanforum.ru
Are you over 18 and want to see adult content?
A complete backup of jonmcnaughton.com
Are you over 18 and want to see adult content?
A complete backup of deniskitchen.com
Are you over 18 and want to see adult content?
Text
ANALYSIS
Boster Bio offers over 16,000 antibodies that have been validated for IHC, WB, ELISA, and FC in human, mouse, and rat samples. Rabbit and mouse monoclonal antibodies as well as rabbit polyclonal antibodies are available. More than 1,000 ELISA kits, both singleplex and multiplex, that have been cited 6,000+ times are available at Boster. PCR SAMPLE PREPARATION Transfer the supernatant to fresh 2 ml tubes. Add 0.1 volume of 3 M sodium acetate and the same volume of isopropanol. Incubate the tubes at -20 °C for 1 h. Spin at top speed for 10 min at 4°C and discard the supernatant. Wash the pellet with 500 μl ethanol (70 %). WESTERN BLOT SAMPLE PREPARATION GUIDE Take out the protein solution in the middle and put it in a fresh tube. Mix the protein solution with Boster SDS-PAGE loading buffer 2X (Catalog# AR0131) in the same volume or with SDS-PAGE loading buffer 5X (Catalog# AR1112) in volume ratio of 4:1 evenly. Denature the solution in 100°C water bath for 5 minutes.IL34 ANTIBODY
100μg/vial. Storage & Handling. Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. Description. Boster Bio Anti-IL34 Antibody Picoband™ catalog # A06903-2. Tested in IHC-P, WBapplications.
ANTI-SYNCYTIN-2 ERVFRD-1 ANTIBODY Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles. Description. Boster Bio Anti-Syncytin-2 ERVFRD-1 Antibody catalog # A10341. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. Supplied as 100ul in Liquid form antibody. NEUTROPHIL ELASTASE/ELA2/ELANE ELISA KIT EK1445. Storage & Handling. Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.) Description. Mouse Neutrophil Elastase/ELA2/ELANE ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Elane in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).. Sensitivity: 10pg/ml. ELISA BLOCKING OPTIMIZATION There are a variety of blocking buffers, not one of which is ideal for every combination of plate type, assay format, and detection system. Every blocking buffer represents a compromise between reducing background and maintaining specificity. Use this guide to help decide which type of blocking buffer is best suited for your specificapplication.
ANTI-SEPIAPTERIN REDUCTASE SPR ANTIBODY Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles. Description. Boster Bio Anti-Sepiapterin reductase SPR Antibody catalog # A00416. Tested in IHC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. Supplied as 100ul in Liquid formantibody.
HOW TO DETERMINE ANTIBODY CROSS-REACTIVITY In order to determine if an antibody validated for human will cross-react with rat, we should take a look at the immunogen sequence (aka antigen sequence) of the antibody. The first step is to copy the provided sequence information and align the sequ IMMUNOHISTOCHEMISTRY (IHC) OPTIMIZATION TIPS Fixation prevents autolysis and necrosis of excised tissues, preserving their morphology and antigenicity as well as increasing their resistance to processing. The choice of fixative is critical as different fixatives are more optimal for some antigens than others. Keywords: IHC, optimization, fixative, pfa, perfusion, immersion,fixation method.
BOSTER BIO | ELISA KITS, ANTIBODIES, ANTIBODY COMPANYSIGN INDISTRIBUTORSABOUT USCONTACT USTISSUE/CELL TYPESTOTAL PROTEINANALYSIS
Boster Bio offers over 16,000 antibodies that have been validated for IHC, WB, ELISA, and FC in human, mouse, and rat samples. Rabbit and mouse monoclonal antibodies as well as rabbit polyclonal antibodies are available. More than 1,000 ELISA kits, both singleplex and multiplex, that have been cited 6,000+ times are available at Boster. PCR SAMPLE PREPARATION Transfer the supernatant to fresh 2 ml tubes. Add 0.1 volume of 3 M sodium acetate and the same volume of isopropanol. Incubate the tubes at -20 °C for 1 h. Spin at top speed for 10 min at 4°C and discard the supernatant. Wash the pellet with 500 μl ethanol (70 %). WESTERN BLOT SAMPLE PREPARATION GUIDE Take out the protein solution in the middle and put it in a fresh tube. Mix the protein solution with Boster SDS-PAGE loading buffer 2X (Catalog# AR0131) in the same volume or with SDS-PAGE loading buffer 5X (Catalog# AR1112) in volume ratio of 4:1 evenly. Denature the solution in 100°C water bath for 5 minutes.IL34 ANTIBODY
100μg/vial. Storage & Handling. Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. Description. Boster Bio Anti-IL34 Antibody Picoband™ catalog # A06903-2. Tested in IHC-P, WBapplications.
ANTI-SYNCYTIN-2 ERVFRD-1 ANTIBODY Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles. Description. Boster Bio Anti-Syncytin-2 ERVFRD-1 Antibody catalog # A10341. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. Supplied as 100ul in Liquid form antibody. NEUTROPHIL ELASTASE/ELA2/ELANE ELISA KIT EK1445. Storage & Handling. Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.) Description. Mouse Neutrophil Elastase/ELA2/ELANE ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Elane in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).. Sensitivity: 10pg/ml. ELISA BLOCKING OPTIMIZATION There are a variety of blocking buffers, not one of which is ideal for every combination of plate type, assay format, and detection system. Every blocking buffer represents a compromise between reducing background and maintaining specificity. Use this guide to help decide which type of blocking buffer is best suited for your specificapplication.
ANTI-SEPIAPTERIN REDUCTASE SPR ANTIBODY Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles. Description. Boster Bio Anti-Sepiapterin reductase SPR Antibody catalog # A00416. Tested in IHC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. Supplied as 100ul in Liquid formantibody.
HOW TO DETERMINE ANTIBODY CROSS-REACTIVITY In order to determine if an antibody validated for human will cross-react with rat, we should take a look at the immunogen sequence (aka antigen sequence) of the antibody. The first step is to copy the provided sequence information and align the sequ IMMUNOHISTOCHEMISTRY (IHC) OPTIMIZATION TIPS Fixation prevents autolysis and necrosis of excised tissues, preserving their morphology and antigenicity as well as increasing their resistance to processing. The choice of fixative is critical as different fixatives are more optimal for some antigens than others. Keywords: IHC, optimization, fixative, pfa, perfusion, immersion,fixation method.
PCR SAMPLE PREPARATION Transfer the supernatant to fresh 2 ml tubes. Add 0.1 volume of 3 M sodium acetate and the same volume of isopropanol. Incubate the tubes at -20 °C for 1 h. Spin at top speed for 10 min at 4°C and discard the supernatant. Wash the pellet with 500 μl ethanol (70 %). FLOW CYTOMETRY SAMPLE PREPARATION Transfer to a chilled centrifuge tube and add ice cold culture medium drop by drop until the cells are diluted 10X. Perform on ice! Centrifuge at 300-400 x g for 5 min at 4°C. Discard supernatant and wash once with cold staining buffer. Re-suspend cells in a suitable volume of cold staining buffer. HOW TO DESIGN POSITIVE AND NEGATIVE CONTROLS Bosterbio, a premium manufacturer of high sensitivity ELISA kits and high quality antibodies ANTI-SYNCYTIN-2 ERVFRD-1 ANTIBODY Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles. Description. Boster Bio Anti-Syncytin-2 ERVFRD-1 Antibody catalog # A10341. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. Supplied as 100ul in Liquid form antibody. QPCR THERMO CYCLER REAL TIME PCR SYSTEM, AZURE CIELO™ 3 AND 6 qPCR Thermo Cycler real time PCR system, Azure Cielo™ 3 and 6. Compatible with the SARS-CoV-2/COVID-19 assays. Viral RNA detection. The Azure Biosystems™ Cielo™ 3 and 6 real-time PCR Systems are designed to offer smart protocols, high quality data, robust durability and an intuitive user experience. WNT SIGNALING PATHWAY Bosterbio, a premium manufacturer of high sensitivity ELISA kits and high quality antibodies HEDGEHOG SIGNALING PATHWAY Bosterbio, a premium manufacturer of high sensitivity ELISA kits and high quality antibodies MOUSE RAT TESTOSTERONE ELISA KIT Mouse Rat Testosterone ELISA Kit. Catalog#. EK7014. Storage & Handling. Store the kit at 2°C to 8°C. Keep microwells sealed in a dry bag with desiccants. The reagents are stable until expiration of the kit. Do not expose reagent to heat, sun, or strong light. Avoid multiple freeze-thaw cycles (Shipped with wet ice.) WESTERN BLOT TROUBLESHOOTING These artifacts are most commonly the result of uneven coating of buffer or antibody, the membrane drying out, or aggregates forming in the antibody or blocking buffer. Learn about Western Blot Principle Western Blot Sample Preparation Check out this ELISA ANTIBODY CONCENTRATION OPTIMIZATION Antibody / Antigen. Concentration. In order to get the best results from your ELISA assay, the dilution factors of the sample and the detection antibodies must be optimized. For strong, quantifiable signal, use a checkerboard titration to test for the optimal concentration of sample and detection antibodies. BOSTER BIO | ELISA KITS, ANTIBODIES, ANTIBODY COMPANYSIGN INDISTRIBUTORSABOUT USCONTACT USTISSUE/CELL TYPESTOTAL PROTEINANALYSIS
Boster Bio offers over 16,000 antibodies that have been validated for IHC, WB, ELISA, and FC in human, mouse, and rat samples. Rabbit and mouse monoclonal antibodies as well as rabbit polyclonal antibodies are available. More than 1,000 ELISA kits, both singleplex and multiplex, that have been cited 6,000+ times are available at Boster. PCR SAMPLE PREPARATION Transfer the supernatant to fresh 2 ml tubes. Add 0.1 volume of 3 M sodium acetate and the same volume of isopropanol. Incubate the tubes at -20 °C for 1 h. Spin at top speed for 10 min at 4°C and discard the supernatant. Wash the pellet with 500 μl ethanol (70 %). WESTERN BLOT SAMPLE PREPARATION GUIDE Take out the protein solution in the middle and put it in a fresh tube. Mix the protein solution with Boster SDS-PAGE loading buffer 2X (Catalog# AR0131) in the same volume or with SDS-PAGE loading buffer 5X (Catalog# AR1112) in volume ratio of 4:1 evenly. Denature the solution in 100°C water bath for 5 minutes. ELISA SAMPLE PREPARATION For every 0.1 mg of tissue, add 500 µL of complete extraction buffer to the tube and homogenize. Rinse the blade of the homogenizer 2X with a 500 µL extraction buffer. Place the sample on a shaker at 4°C for 1 hour. Centrifuge the sample at approximately 10000 X g for 5 min. Assay immediately or aliquot supernatant (soluble protein extract FLOW CYTOMETRY SAMPLE PREPARATION Transfer to a chilled centrifuge tube and add ice cold culture medium drop by drop until the cells are diluted 10X. Perform on ice! Centrifuge at 300-400 x g for 5 min at 4°C. Discard supernatant and wash once with cold staining buffer. Re-suspend cells in a suitable volume of cold staining buffer. ANTI-SYNCYTIN-2 ERVFRD-1 ANTIBODY Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles. Description. Boster Bio Anti-Syncytin-2 ERVFRD-1 Antibody catalog # A10341. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. Supplied as 100ul in Liquid form antibody. MOUSE RAT TESTOSTERONE ELISA KIT Mouse Rat Testosterone ELISA Kit. Catalog#. EK7014. Storage & Handling. Store the kit at 2°C to 8°C. Keep microwells sealed in a dry bag with desiccants. The reagents are stable until expiration of the kit. Do not expose reagent to heat, sun, or strong light. Avoid multiple freeze-thaw cycles (Shipped with wet ice.) IMMUNOHISTOCHEMISTRY PRINCIPLE & PROTOCOL Immunohistochemistry (IHC) is a method for detecting antigens or haptens in cells of a tissue section by exploiting the principle of antibodies binding specifically to antigens in biological tissues. The antibody-antigen binding can be visualized in different manners. Enzymes, such as Horseradish Peroxidase (HRP) or Alkaline Phosphatase(AP
ELISA PLATE WASHING OPTIMIZATION ELISA Plate Washing Optimization. One of the defining features of an ELISA assay is the ability to quickly and effectively wash away unbound molecules. This allows ELISA assays to capture specific antigens or antibodies from crude samples, enabling researchers to identify, isolate, and STAT4 LUCIFERASE REPORTER-BA/F3 CELL LINE The STAT4 Luciferase Reporter cell line is a stably transfected Ba/F3 cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the STAT4 responsive promoter, so that the cell line is designed to measure the transcriptional activity of STAT4. Signal Transducer and Activator of Transcription 4 (STAT4) is a BOSTER BIO | ELISA KITS, ANTIBODIES, ANTIBODY COMPANYSIGN INDISTRIBUTORSABOUT USCONTACT USTISSUE/CELL TYPESTOTAL PROTEINANALYSIS
Boster Bio offers over 16,000 antibodies that have been validated for IHC, WB, ELISA, and FC in human, mouse, and rat samples. Rabbit and mouse monoclonal antibodies as well as rabbit polyclonal antibodies are available. More than 1,000 ELISA kits, both singleplex and multiplex, that have been cited 6,000+ times are available at Boster. PCR SAMPLE PREPARATION Transfer the supernatant to fresh 2 ml tubes. Add 0.1 volume of 3 M sodium acetate and the same volume of isopropanol. Incubate the tubes at -20 °C for 1 h. Spin at top speed for 10 min at 4°C and discard the supernatant. Wash the pellet with 500 μl ethanol (70 %). WESTERN BLOT SAMPLE PREPARATION GUIDE Take out the protein solution in the middle and put it in a fresh tube. Mix the protein solution with Boster SDS-PAGE loading buffer 2X (Catalog# AR0131) in the same volume or with SDS-PAGE loading buffer 5X (Catalog# AR1112) in volume ratio of 4:1 evenly. Denature the solution in 100°C water bath for 5 minutes. ELISA SAMPLE PREPARATION For every 0.1 mg of tissue, add 500 µL of complete extraction buffer to the tube and homogenize. Rinse the blade of the homogenizer 2X with a 500 µL extraction buffer. Place the sample on a shaker at 4°C for 1 hour. Centrifuge the sample at approximately 10000 X g for 5 min. Assay immediately or aliquot supernatant (soluble protein extract FLOW CYTOMETRY SAMPLE PREPARATION Transfer to a chilled centrifuge tube and add ice cold culture medium drop by drop until the cells are diluted 10X. Perform on ice! Centrifuge at 300-400 x g for 5 min at 4°C. Discard supernatant and wash once with cold staining buffer. Re-suspend cells in a suitable volume of cold staining buffer. ANTI-SYNCYTIN-2 ERVFRD-1 ANTIBODY Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles. Description. Boster Bio Anti-Syncytin-2 ERVFRD-1 Antibody catalog # A10341. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. Supplied as 100ul in Liquid form antibody. MOUSE RAT TESTOSTERONE ELISA KIT Mouse Rat Testosterone ELISA Kit. Catalog#. EK7014. Storage & Handling. Store the kit at 2°C to 8°C. Keep microwells sealed in a dry bag with desiccants. The reagents are stable until expiration of the kit. Do not expose reagent to heat, sun, or strong light. Avoid multiple freeze-thaw cycles (Shipped with wet ice.) IMMUNOHISTOCHEMISTRY PRINCIPLE & PROTOCOL Immunohistochemistry (IHC) is a method for detecting antigens or haptens in cells of a tissue section by exploiting the principle of antibodies binding specifically to antigens in biological tissues. The antibody-antigen binding can be visualized in different manners. Enzymes, such as Horseradish Peroxidase (HRP) or Alkaline Phosphatase(AP
ELISA PLATE WASHING OPTIMIZATION ELISA Plate Washing Optimization. One of the defining features of an ELISA assay is the ability to quickly and effectively wash away unbound molecules. This allows ELISA assays to capture specific antigens or antibodies from crude samples, enabling researchers to identify, isolate, and STAT4 LUCIFERASE REPORTER-BA/F3 CELL LINE The STAT4 Luciferase Reporter cell line is a stably transfected Ba/F3 cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the STAT4 responsive promoter, so that the cell line is designed to measure the transcriptional activity of STAT4. Signal Transducer and Activator of Transcription 4 (STAT4) is a HOW TO DESIGN POSITIVE AND NEGATIVE CONTROLS Bosterbio, a premium manufacturer of high sensitivity ELISA kits and high quality antibodies IMMUNOHISTOCHEMISTRY PRINCIPLE & PROTOCOL Immunohistochemistry (IHC) is a method for detecting antigens or haptens in cells of a tissue section by exploiting the principle of antibodies binding specifically to antigens in biological tissues. The antibody-antigen binding can be visualized in different manners. Enzymes, such as Horseradish Peroxidase (HRP) or Alkaline Phosphatase(AP
ELISA BLOCKING OPTIMIZATION There are a variety of blocking buffers, not one of which is ideal for every combination of plate type, assay format, and detection system. Every blocking buffer represents a compromise between reducing background and maintaining specificity. Use this guide to help decide which type of blocking buffer is best suited for your specificapplication.
IL34 ANTIBODY
100μg/vial. Storage & Handling. Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. Description. Boster Bio Anti-IL34 Antibody Picoband™ catalog # A06903-2. Tested in IHC-P, WBapplications.
ANTI-SYNCYTIN-2 ERVFRD-1 ANTIBODY Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles. Description. Boster Bio Anti-Syncytin-2 ERVFRD-1 Antibody catalog # A10341. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. Supplied as 100ul in Liquid form antibody. ELISA FUNDAMENTAL PRINCIPLE, HOW IT WORKS ELISA (enzyme-linked immunosorbent assay) is a plate-based assay technique designed for detecting and quantifying peptides, proteins, antibodies, and hormones. In ELISA, an antigen must be immobilized to a solid surface and then complexed with an antibody that is linked toan enzyme.
IMMUNOHISTOCHEMISTRY (IHC) TROUBLESHOOTING GUIDE This guide provides a thorough list of Immunohistochemistry troubleshooting tips, including weak staining, high background, nonspecific staining among others. Learn how HEDGEHOG SIGNALING PATHWAY Bosterbio, a premium manufacturer of high sensitivity ELISA kits and high quality antibodies WESTERN BLOT TROUBLESHOOTING Follow the tips below to identify and solve the cause of your imperfect western blot background. S.No. Possible Cause. Solution. 1. Blotched background: Uneven antibody distribution. Agitate during incubation to coat the membrane evenly in incubation buffer. 2. FOCUSGEN INFLUENZA VIRUS AND SARS-COV-2 MULTIPLEX ONESTEP FocusGen Influenza Virus and SARS-CoV-2 Multiplex OneStep RT PCR Test Kit - No Need For Viral RNA Extraction Intended Use Influenza virus (IFV) A (a) and B (b) are the most common respiratory viruses in winter and spring. Detection of influenza virus and BOSTER BIO | ELISA KITS, ANTIBODIES, ANTIBODY COMPANYSIGN INDISTRIBUTORSABOUT USCONTACT USTISSUE/CELL TYPESTOTAL PROTEINANALYSIS
Boster Bio offers over 16,000 antibodies that have been validated for IHC, WB, ELISA, and FC in human, mouse, and rat samples. Rabbit and mouse monoclonal antibodies as well as rabbit polyclonal antibodies are available. More than 1,000 ELISA kits, both singleplex and multiplex, that have been cited 6,000+ times are available at Boster. PCR SAMPLE PREPARATION Transfer the supernatant to fresh 2 ml tubes. Add 0.1 volume of 3 M sodium acetate and the same volume of isopropanol. Incubate the tubes at -20 °C for 1 h. Spin at top speed for 10 min at 4°C and discard the supernatant. Wash the pellet with 500 μl ethanol (70 %). ELISA SAMPLE PREPARATION For every 0.1 mg of tissue, add 500 µL of complete extraction buffer to the tube and homogenize. Rinse the blade of the homogenizer 2X with a 500 µL extraction buffer. Place the sample on a shaker at 4°C for 1 hour. Centrifuge the sample at approximately 10000 X g for 5 min. Assay immediately or aliquot supernatant (soluble protein extract WESTERN BLOT SAMPLE PREPARATION GUIDE Take out the protein solution in the middle and put it in a fresh tube. Mix the protein solution with Boster SDS-PAGE loading buffer 2X (Catalog# AR0131) in the same volume or with SDS-PAGE loading buffer 5X (Catalog# AR1112) in volume ratio of 4:1 evenly. Denature the solution in 100°C water bath for 5 minutes. FLOW CYTOMETRY SAMPLE PREPARATION Transfer to a chilled centrifuge tube and add ice cold culture medium drop by drop until the cells are diluted 10X. Perform on ice! Centrifuge at 300-400 x g for 5 min at 4°C. Discard supernatant and wash once with cold staining buffer. Re-suspend cells in a suitable volume of cold staining buffer. ANTI-SYNCYTIN-2 ERVFRD-1 ANTIBODY Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles. Description. Boster Bio Anti-Syncytin-2 ERVFRD-1 Antibody catalog # A10341. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. Supplied as 100ul in Liquid form antibody. IMMUNOHISTOCHEMISTRY PRINCIPLE & PROTOCOL Immunohistochemistry (IHC) is a method for detecting antigens or haptens in cells of a tissue section by exploiting the principle of antibodies binding specifically to antigens in biological tissues. The antibody-antigen binding can be visualized in different manners. Enzymes, such as Horseradish Peroxidase (HRP) or Alkaline Phosphatase(AP
HOW TO DETERMINE ANTIBODY CROSS-REACTIVITY We recommend an alignment score of over 85% as a good indication that an antibody may cross-react. For example, if the homology of the human sequence and rat sequence is higher than 90%, then, in theory, the antibody will cross-react with rat. The following image shows an example of what the results of a sequence alignment might look like. ELISA PLATE WASHING OPTIMIZATION ELISA Plate Washing Optimization. One of the defining features of an ELISA assay is the ability to quickly and effectively wash away unbound molecules. This allows ELISA assays to capture specific antigens or antibodies from crude samples, enabling researchers to identify, isolate, and STAT4 LUCIFERASE REPORTER-BA/F3 CELL LINE The STAT4 Luciferase Reporter cell line is a stably transfected Ba/F3 cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the STAT4 responsive promoter, so that the cell line is designed to measure the transcriptional activity of STAT4. Signal Transducer and Activator of Transcription 4 (STAT4) is a BOSTER BIO | ELISA KITS, ANTIBODIES, ANTIBODY COMPANYSIGN INDISTRIBUTORSABOUT USCONTACT USTISSUE/CELL TYPESTOTAL PROTEINANALYSIS
Boster Bio offers over 16,000 antibodies that have been validated for IHC, WB, ELISA, and FC in human, mouse, and rat samples. Rabbit and mouse monoclonal antibodies as well as rabbit polyclonal antibodies are available. More than 1,000 ELISA kits, both singleplex and multiplex, that have been cited 6,000+ times are available at Boster. PCR SAMPLE PREPARATION Transfer the supernatant to fresh 2 ml tubes. Add 0.1 volume of 3 M sodium acetate and the same volume of isopropanol. Incubate the tubes at -20 °C for 1 h. Spin at top speed for 10 min at 4°C and discard the supernatant. Wash the pellet with 500 μl ethanol (70 %). ELISA SAMPLE PREPARATION For every 0.1 mg of tissue, add 500 µL of complete extraction buffer to the tube and homogenize. Rinse the blade of the homogenizer 2X with a 500 µL extraction buffer. Place the sample on a shaker at 4°C for 1 hour. Centrifuge the sample at approximately 10000 X g for 5 min. Assay immediately or aliquot supernatant (soluble protein extract WESTERN BLOT SAMPLE PREPARATION GUIDE Take out the protein solution in the middle and put it in a fresh tube. Mix the protein solution with Boster SDS-PAGE loading buffer 2X (Catalog# AR0131) in the same volume or with SDS-PAGE loading buffer 5X (Catalog# AR1112) in volume ratio of 4:1 evenly. Denature the solution in 100°C water bath for 5 minutes. FLOW CYTOMETRY SAMPLE PREPARATION Transfer to a chilled centrifuge tube and add ice cold culture medium drop by drop until the cells are diluted 10X. Perform on ice! Centrifuge at 300-400 x g for 5 min at 4°C. Discard supernatant and wash once with cold staining buffer. Re-suspend cells in a suitable volume of cold staining buffer. ANTI-SYNCYTIN-2 ERVFRD-1 ANTIBODY Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles. Description. Boster Bio Anti-Syncytin-2 ERVFRD-1 Antibody catalog # A10341. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. Supplied as 100ul in Liquid form antibody. IMMUNOHISTOCHEMISTRY PRINCIPLE & PROTOCOL Immunohistochemistry (IHC) is a method for detecting antigens or haptens in cells of a tissue section by exploiting the principle of antibodies binding specifically to antigens in biological tissues. The antibody-antigen binding can be visualized in different manners. Enzymes, such as Horseradish Peroxidase (HRP) or Alkaline Phosphatase(AP
HOW TO DETERMINE ANTIBODY CROSS-REACTIVITY We recommend an alignment score of over 85% as a good indication that an antibody may cross-react. For example, if the homology of the human sequence and rat sequence is higher than 90%, then, in theory, the antibody will cross-react with rat. The following image shows an example of what the results of a sequence alignment might look like. ELISA PLATE WASHING OPTIMIZATION ELISA Plate Washing Optimization. One of the defining features of an ELISA assay is the ability to quickly and effectively wash away unbound molecules. This allows ELISA assays to capture specific antigens or antibodies from crude samples, enabling researchers to identify, isolate, and STAT4 LUCIFERASE REPORTER-BA/F3 CELL LINE The STAT4 Luciferase Reporter cell line is a stably transfected Ba/F3 cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the STAT4 responsive promoter, so that the cell line is designed to measure the transcriptional activity of STAT4. Signal Transducer and Activator of Transcription 4 (STAT4) is a HOW TO DESIGN POSITIVE AND NEGATIVE CONTROLS Bosterbio, a premium manufacturer of high sensitivity ELISA kits and high quality antibodies MOUSE RAT TESTOSTERONE ELISA KIT Mouse Rat Testosterone ELISA Kit. Catalog#. EK7014. Storage & Handling. Store the kit at 2°C to 8°C. Keep microwells sealed in a dry bag with desiccants. The reagents are stable until expiration of the kit. Do not expose reagent to heat, sun, or strong light. Avoid multiple freeze-thaw cycles (Shipped with wet ice.) HEDGEHOG SIGNALING PATHWAY Bosterbio, a premium manufacturer of high sensitivity ELISA kits and high quality antibodies ELISA BLOCKING OPTIMIZATION There are a variety of blocking buffers, not one of which is ideal for every combination of plate type, assay format, and detection system. Every blocking buffer represents a compromise between reducing background and maintaining specificity. Use this guide to help decide which type of blocking buffer is best suited for your specificapplication.
ANTI-SYNCYTIN-2 ERVFRD-1 ANTIBODY Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles. Description. Boster Bio Anti-Syncytin-2 ERVFRD-1 Antibody catalog # A10341. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. Supplied as 100ul in Liquid form antibody. FOCUSGEN INFLUENZA VIRUS AND SARS-COV-2 MULTIPLEX ONESTEP FocusGen Influenza Virus and SARS-CoV-2 Multiplex OneStep RT PCR Test Kit - No Need For Viral RNA Extraction Intended Use Influenza virus (IFV) A (a) and B (b) are the most common respiratory viruses in winter and spring. Detection of influenza virus and IMMUNOHISTOCHEMISTRY (IHC) OPTIMIZATION TIPS Fixation prevents autolysis and necrosis of excised tissues, preserving their morphology and antigenicity as well as increasing their resistance to processing. The choice of fixative is critical as different fixatives are more optimal for some antigens than others. Keywords: IHC, optimization, fixative, pfa, perfusion, immersion,fixation method.
WESTERN BLOT TROUBLESHOOTING Follow the tips below to identify and solve the cause of your imperfect western blot background. S.No. Possible Cause. Solution. 1. Blotched background: Uneven antibody distribution. Agitate during incubation to coat the membrane evenly in incubation buffer. 2. ELISA PLATE WASHING OPTIMIZATION ELISA Plate Washing Optimization. One of the defining features of an ELISA assay is the ability to quickly and effectively wash away unbound molecules. This allows ELISA assays to capture specific antigens or antibodies from crude samples, enabling researchers to identify, isolate, andFACS DATA ANALYSIS
Back-gating is a method to confirm a gating pattern. The population that has been identified by a particular gate is gated again on entirely different parameters. It is usually done when one is trying out a new gating strategy or there is a concern of non-specific staining and false positives. In the dot plots shown above, bloodcells are first
BOSTER BIO | ELISA KITS, ANTIBODIES, ANTIBODY COMPANYSIGN INDISTRIBUTORSABOUT USCONTACT USTISSUE/CELL TYPESTOTAL PROTEINANALYSIS
Boster Bio offers over 16,000 antibodies that have been validated for IHC, WB, ELISA, and FC in human, mouse, and rat samples. Rabbit and mouse monoclonal antibodies as well as rabbit polyclonal antibodies are available. More than 1,000 ELISA kits, both singleplex and multiplex, that have been cited 6,000+ times are available at Boster. PCR SAMPLE PREPARATION Transfer the supernatant to fresh 2 ml tubes. Add 0.1 volume of 3 M sodium acetate and the same volume of isopropanol. Incubate the tubes at -20 °C for 1 h. Spin at top speed for 10 min at 4°C and discard the supernatant. Wash the pellet with 500 μl ethanol (70 %). ELISA SAMPLE PREPARATION For every 0.1 mg of tissue, add 500 µL of complete extraction buffer to the tube and homogenize. Rinse the blade of the homogenizer 2X with a 500 µL extraction buffer. Place the sample on a shaker at 4°C for 1 hour. Centrifuge the sample at approximately 10000 X g for 5 min. Assay immediately or aliquot supernatant (soluble protein extract WESTERN BLOT SAMPLE PREPARATION GUIDE Take out the protein solution in the middle and put it in a fresh tube. Mix the protein solution with Boster SDS-PAGE loading buffer 2X (Catalog# AR0131) in the same volume or with SDS-PAGE loading buffer 5X (Catalog# AR1112) in volume ratio of 4:1 evenly. Denature the solution in 100°C water bath for 5 minutes. FLOW CYTOMETRY SAMPLE PREPARATION Transfer to a chilled centrifuge tube and add ice cold culture medium drop by drop until the cells are diluted 10X. Perform on ice! Centrifuge at 300-400 x g for 5 min at 4°C. Discard supernatant and wash once with cold staining buffer. Re-suspend cells in a suitable volume of cold staining buffer. ANTI-SYNCYTIN-2 ERVFRD-1 ANTIBODY Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles. Description. Boster Bio Anti-Syncytin-2 ERVFRD-1 Antibody catalog # A10341. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. Supplied as 100ul in Liquid form antibody. IMMUNOHISTOCHEMISTRY PRINCIPLE & PROTOCOL Immunohistochemistry (IHC) is a method for detecting antigens or haptens in cells of a tissue section by exploiting the principle of antibodies binding specifically to antigens in biological tissues. The antibody-antigen binding can be visualized in different manners. Enzymes, such as Horseradish Peroxidase (HRP) or Alkaline Phosphatase(AP
HOW TO DETERMINE ANTIBODY CROSS-REACTIVITY We recommend an alignment score of over 85% as a good indication that an antibody may cross-react. For example, if the homology of the human sequence and rat sequence is higher than 90%, then, in theory, the antibody will cross-react with rat. The following image shows an example of what the results of a sequence alignment might look like. ELISA PLATE WASHING OPTIMIZATION ELISA Plate Washing Optimization. One of the defining features of an ELISA assay is the ability to quickly and effectively wash away unbound molecules. This allows ELISA assays to capture specific antigens or antibodies from crude samples, enabling researchers to identify, isolate, and STAT4 LUCIFERASE REPORTER-BA/F3 CELL LINE The STAT4 Luciferase Reporter cell line is a stably transfected Ba/F3 cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the STAT4 responsive promoter, so that the cell line is designed to measure the transcriptional activity of STAT4. Signal Transducer and Activator of Transcription 4 (STAT4) is a BOSTER BIO | ELISA KITS, ANTIBODIES, ANTIBODY COMPANYSIGN INDISTRIBUTORSABOUT USCONTACT USTISSUE/CELL TYPESTOTAL PROTEINANALYSIS
Boster Bio offers over 16,000 antibodies that have been validated for IHC, WB, ELISA, and FC in human, mouse, and rat samples. Rabbit and mouse monoclonal antibodies as well as rabbit polyclonal antibodies are available. More than 1,000 ELISA kits, both singleplex and multiplex, that have been cited 6,000+ times are available at Boster. PCR SAMPLE PREPARATION Transfer the supernatant to fresh 2 ml tubes. Add 0.1 volume of 3 M sodium acetate and the same volume of isopropanol. Incubate the tubes at -20 °C for 1 h. Spin at top speed for 10 min at 4°C and discard the supernatant. Wash the pellet with 500 μl ethanol (70 %). ELISA SAMPLE PREPARATION For every 0.1 mg of tissue, add 500 µL of complete extraction buffer to the tube and homogenize. Rinse the blade of the homogenizer 2X with a 500 µL extraction buffer. Place the sample on a shaker at 4°C for 1 hour. Centrifuge the sample at approximately 10000 X g for 5 min. Assay immediately or aliquot supernatant (soluble protein extract WESTERN BLOT SAMPLE PREPARATION GUIDE Take out the protein solution in the middle and put it in a fresh tube. Mix the protein solution with Boster SDS-PAGE loading buffer 2X (Catalog# AR0131) in the same volume or with SDS-PAGE loading buffer 5X (Catalog# AR1112) in volume ratio of 4:1 evenly. Denature the solution in 100°C water bath for 5 minutes. FLOW CYTOMETRY SAMPLE PREPARATION Transfer to a chilled centrifuge tube and add ice cold culture medium drop by drop until the cells are diluted 10X. Perform on ice! Centrifuge at 300-400 x g for 5 min at 4°C. Discard supernatant and wash once with cold staining buffer. Re-suspend cells in a suitable volume of cold staining buffer. ANTI-SYNCYTIN-2 ERVFRD-1 ANTIBODY Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles. Description. Boster Bio Anti-Syncytin-2 ERVFRD-1 Antibody catalog # A10341. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. Supplied as 100ul in Liquid form antibody. IMMUNOHISTOCHEMISTRY PRINCIPLE & PROTOCOL Immunohistochemistry (IHC) is a method for detecting antigens or haptens in cells of a tissue section by exploiting the principle of antibodies binding specifically to antigens in biological tissues. The antibody-antigen binding can be visualized in different manners. Enzymes, such as Horseradish Peroxidase (HRP) or Alkaline Phosphatase(AP
HOW TO DETERMINE ANTIBODY CROSS-REACTIVITY We recommend an alignment score of over 85% as a good indication that an antibody may cross-react. For example, if the homology of the human sequence and rat sequence is higher than 90%, then, in theory, the antibody will cross-react with rat. The following image shows an example of what the results of a sequence alignment might look like. ELISA PLATE WASHING OPTIMIZATION ELISA Plate Washing Optimization. One of the defining features of an ELISA assay is the ability to quickly and effectively wash away unbound molecules. This allows ELISA assays to capture specific antigens or antibodies from crude samples, enabling researchers to identify, isolate, and STAT4 LUCIFERASE REPORTER-BA/F3 CELL LINE The STAT4 Luciferase Reporter cell line is a stably transfected Ba/F3 cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the STAT4 responsive promoter, so that the cell line is designed to measure the transcriptional activity of STAT4. Signal Transducer and Activator of Transcription 4 (STAT4) is a HOW TO DESIGN POSITIVE AND NEGATIVE CONTROLS Bosterbio, a premium manufacturer of high sensitivity ELISA kits and high quality antibodies MOUSE RAT TESTOSTERONE ELISA KIT Mouse Rat Testosterone ELISA Kit. Catalog#. EK7014. Storage & Handling. Store the kit at 2°C to 8°C. Keep microwells sealed in a dry bag with desiccants. The reagents are stable until expiration of the kit. Do not expose reagent to heat, sun, or strong light. Avoid multiple freeze-thaw cycles (Shipped with wet ice.) HEDGEHOG SIGNALING PATHWAY Bosterbio, a premium manufacturer of high sensitivity ELISA kits and high quality antibodies ELISA BLOCKING OPTIMIZATION There are a variety of blocking buffers, not one of which is ideal for every combination of plate type, assay format, and detection system. Every blocking buffer represents a compromise between reducing background and maintaining specificity. Use this guide to help decide which type of blocking buffer is best suited for your specificapplication.
ANTI-SYNCYTIN-2 ERVFRD-1 ANTIBODY Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles. Description. Boster Bio Anti-Syncytin-2 ERVFRD-1 Antibody catalog # A10341. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. Supplied as 100ul in Liquid form antibody. FOCUSGEN INFLUENZA VIRUS AND SARS-COV-2 MULTIPLEX ONESTEP FocusGen Influenza Virus and SARS-CoV-2 Multiplex OneStep RT PCR Test Kit - No Need For Viral RNA Extraction Intended Use Influenza virus (IFV) A (a) and B (b) are the most common respiratory viruses in winter and spring. Detection of influenza virus and IMMUNOHISTOCHEMISTRY (IHC) OPTIMIZATION TIPS Fixation prevents autolysis and necrosis of excised tissues, preserving their morphology and antigenicity as well as increasing their resistance to processing. The choice of fixative is critical as different fixatives are more optimal for some antigens than others. Keywords: IHC, optimization, fixative, pfa, perfusion, immersion,fixation method.
WESTERN BLOT TROUBLESHOOTING Follow the tips below to identify and solve the cause of your imperfect western blot background. S.No. Possible Cause. Solution. 1. Blotched background: Uneven antibody distribution. Agitate during incubation to coat the membrane evenly in incubation buffer. 2. ELISA PLATE WASHING OPTIMIZATION ELISA Plate Washing Optimization. One of the defining features of an ELISA assay is the ability to quickly and effectively wash away unbound molecules. This allows ELISA assays to capture specific antigens or antibodies from crude samples, enabling researchers to identify, isolate, andFACS DATA ANALYSIS
Back-gating is a method to confirm a gating pattern. The population that has been identified by a particular gate is gated again on entirely different parameters. It is usually done when one is trying out a new gating strategy or there is a concern of non-specific staining and false positives. In the dot plots shown above, bloodcells are first
BOSTER BIO | ELISA KITS, ANTIBODIES, ANTIBODY COMPANYSIGN INDISTRIBUTORSABOUT USCONTACT USTISSUE/CELL TYPESTOTAL PROTEINANALYSIS
Boster Bio offers over 16,000 antibodies that have been validated for IHC, WB, ELISA, and FC in human, mouse, and rat samples. Rabbit and mouse monoclonal antibodies as well as rabbit polyclonal antibodies are available. More than 1,000 ELISA kits, both singleplex and multiplex, that have been cited 6,000+ times are available at Boster.IL34 ANTIBODY
100μg/vial. Storage & Handling. Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. Description. Boster Bio Anti-IL34 Antibody Picoband™ catalog # A06903-2. Tested in IHC-P, WBapplications.
WESTERN BLOT SAMPLE PREPARATION GUIDE Take out the protein solution in the middle and put it in a fresh tube. Mix the protein solution with Boster SDS-PAGE loading buffer 2X (Catalog# AR0131) in the same volume or with SDS-PAGE loading buffer 5X (Catalog# AR1112) in volume ratio of 4:1 evenly. Denature the solution in 100°C water bath for 5 minutes. ELISA BLOCKING OPTIMIZATION There are a variety of blocking buffers, not one of which is ideal for every combination of plate type, assay format, and detection system. Every blocking buffer represents a compromise between reducing background and maintaining specificity. Use this guide to help decide which type of blocking buffer is best suited for your specificapplication.
FLOW CYTOMETRY SAMPLE PREPARATION Transfer to a chilled centrifuge tube and add ice cold culture medium drop by drop until the cells are diluted 10X. Perform on ice! Centrifuge at 300-400 x g for 5 min at 4°C. Discard supernatant and wash once with cold staining buffer. Re-suspend cells in a suitable volume of cold staining buffer. MOUSE RAT TESTOSTERONE ELISA KIT Mouse Rat Testosterone ELISA Kit. Catalog#. EK7014. Storage & Handling. Store the kit at 2°C to 8°C. Keep microwells sealed in a dry bag with desiccants. The reagents are stable until expiration of the kit. Do not expose reagent to heat, sun, or strong light. Avoid multiple freeze-thaw cycles (Shipped with wet ice.) IMMUNOHISTOCHEMISTRY PRINCIPLE & PROTOCOL Immunohistochemistry (IHC) is a method for detecting antigens or haptens in cells of a tissue section by exploiting the principle of antibodies binding specifically to antigens in biological tissues. The antibody-antigen binding can be visualized in different manners. Enzymes, such as Horseradish Peroxidase (HRP) or Alkaline Phosphatase(AP
HOW TO DETERMINE ANTIBODY CROSS-REACTIVITYHOW TO DETERMINE REACTIVITYELEMENTS
We recommend an alignment score of over 85% as a good indication that an antibody may cross-react. For example, if the homology of the human sequence and rat sequence is higher than 90%, then, in theory, the antibody will cross-react with rat. The following image shows an example of what the results of a sequence alignment might look like. ANTI-SEPIAPTERIN REDUCTASE SPR ANTIBODY Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles. Description. Boster Bio Anti-Sepiapterin reductase SPR Antibody catalog # A00416. Tested in IHC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. Supplied as 100ul in Liquid formantibody.
SDS-PAGE OPTIMIZATION SDS-PAGE is the most widely used method for separating proteins by their relative molecular weights. By denaturing proteins in the presence of SDS, an ionic detergent, proteins can be linearized and imbued with a negative charge. This allows for an electric field to press them through the polyacrylamide gel matrix. BOSTER BIO | ELISA KITS, ANTIBODIES, ANTIBODY COMPANYSIGN INDISTRIBUTORSABOUT USCONTACT USTISSUE/CELL TYPESTOTAL PROTEINANALYSIS
Boster Bio offers over 16,000 antibodies that have been validated for IHC, WB, ELISA, and FC in human, mouse, and rat samples. Rabbit and mouse monoclonal antibodies as well as rabbit polyclonal antibodies are available. More than 1,000 ELISA kits, both singleplex and multiplex, that have been cited 6,000+ times are available at Boster.IL34 ANTIBODY
100μg/vial. Storage & Handling. Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. Description. Boster Bio Anti-IL34 Antibody Picoband™ catalog # A06903-2. Tested in IHC-P, WBapplications.
WESTERN BLOT SAMPLE PREPARATION GUIDE Take out the protein solution in the middle and put it in a fresh tube. Mix the protein solution with Boster SDS-PAGE loading buffer 2X (Catalog# AR0131) in the same volume or with SDS-PAGE loading buffer 5X (Catalog# AR1112) in volume ratio of 4:1 evenly. Denature the solution in 100°C water bath for 5 minutes. ELISA BLOCKING OPTIMIZATION There are a variety of blocking buffers, not one of which is ideal for every combination of plate type, assay format, and detection system. Every blocking buffer represents a compromise between reducing background and maintaining specificity. Use this guide to help decide which type of blocking buffer is best suited for your specificapplication.
FLOW CYTOMETRY SAMPLE PREPARATION Transfer to a chilled centrifuge tube and add ice cold culture medium drop by drop until the cells are diluted 10X. Perform on ice! Centrifuge at 300-400 x g for 5 min at 4°C. Discard supernatant and wash once with cold staining buffer. Re-suspend cells in a suitable volume of cold staining buffer. MOUSE RAT TESTOSTERONE ELISA KIT Mouse Rat Testosterone ELISA Kit. Catalog#. EK7014. Storage & Handling. Store the kit at 2°C to 8°C. Keep microwells sealed in a dry bag with desiccants. The reagents are stable until expiration of the kit. Do not expose reagent to heat, sun, or strong light. Avoid multiple freeze-thaw cycles (Shipped with wet ice.) IMMUNOHISTOCHEMISTRY PRINCIPLE & PROTOCOL Immunohistochemistry (IHC) is a method for detecting antigens or haptens in cells of a tissue section by exploiting the principle of antibodies binding specifically to antigens in biological tissues. The antibody-antigen binding can be visualized in different manners. Enzymes, such as Horseradish Peroxidase (HRP) or Alkaline Phosphatase(AP
HOW TO DETERMINE ANTIBODY CROSS-REACTIVITYHOW TO DETERMINE REACTIVITYELEMENTS
We recommend an alignment score of over 85% as a good indication that an antibody may cross-react. For example, if the homology of the human sequence and rat sequence is higher than 90%, then, in theory, the antibody will cross-react with rat. The following image shows an example of what the results of a sequence alignment might look like. ANTI-SEPIAPTERIN REDUCTASE SPR ANTIBODY Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles. Description. Boster Bio Anti-Sepiapterin reductase SPR Antibody catalog # A00416. Tested in IHC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. Supplied as 100ul in Liquid formantibody.
SDS-PAGE OPTIMIZATION SDS-PAGE is the most widely used method for separating proteins by their relative molecular weights. By denaturing proteins in the presence of SDS, an ionic detergent, proteins can be linearized and imbued with a negative charge. This allows for an electric field to press them through the polyacrylamide gel matrix. MOUSE RAT TESTOSTERONE ELISA KIT Mouse Rat Testosterone ELISA Kit. Catalog#. EK7014. Storage & Handling. Store the kit at 2°C to 8°C. Keep microwells sealed in a dry bag with desiccants. The reagents are stable until expiration of the kit. Do not expose reagent to heat, sun, or strong light. Avoid multiple freeze-thaw cycles (Shipped with wet ice.) ELISA FUNDAMENTAL PRINCIPLE, HOW IT WORKS ELISA (enzyme-linked immunosorbent assay) is a plate-based assay technique designed for detecting and quantifying peptides, proteins, antibodies, and hormones. In ELISA, an antigen must be immobilized to a solid surface and then complexed with an antibody that is linked toan enzyme.
ANTI-SYNCYTIN-2 ERVFRD-1 ANTIBODY Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles. Description. Boster Bio Anti-Syncytin-2 ERVFRD-1 Antibody catalog # A10341. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. Supplied as 100ul in Liquid form antibody.NUCLEAR FAST RED
Boster’s Nuclear Fast Red is an optimized nuclear counterstain solution that produces an intense nuclear stain. It is an anionic dye used in conjunction with an aluminium salt as a mordant. In contrast to hematoxylin, Nuclear Fast Red is a rapid staining procedure, whichrequires only
ANTI-MAP3KL4 ANTIBODY (A30507) For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles. Description. Boster Bio Anti-MAP3KL4 MAP3K21 Antibody catalog # A30507. Tested in WB applications. This antibody reacts with Human. Supplied as ANTI-USP19 ANTIBODY A05870 Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles. Description. Boster Bio Anti-USP19 Antibody catalog # A05870. Tested in IHC, WB applications. This antibody reacts with WESTERN BLOTTING PRINCIPLE Western Blotting (also called immunoblotting) is a technique used for analysis of individual proteins in a protein mixture (e.g. a cell lysate). The proteins on this immunoblot are then accessible for antibody binding for detection. Antibodies are used to detect target proteins on the western blot (immunoblot). Q4. DENTINOGENESIS RELATED GENES AND FACTS Quite a number of genes have been found to play important roles in Dentinogenesis, such as ALPP, ATRNL1, Asrgl1, BGLAP, CCL27, COL1A1, COL1A2, CXXC1, DMP1, DMTF1, DSP, DSPP, FN1, IBSP, PDLIM3, RUNX2, SLC4A1, SLPI, SPP1. See what Boster has to offer for the research of these genes by clicking the gene name links below and view a moredetailed
ELISA ANTIBODY CONCENTRATION OPTIMIZATION Antibody / Antigen. Concentration. In order to get the best results from your ELISA assay, the dilution factors of the sample and the detection antibodies must be optimized. For strong, quantifiable signal, use a checkerboard titration to test for the optimal concentration of sample and detection antibodies. STAT4 LUCIFERASE REPORTER-BA/F3 CELL LINE The STAT4 Luciferase Reporter cell line is a stably transfected Ba/F3 cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the STAT4 responsive promoter, so that the cell line is designed to measure the transcriptional activity of STAT4. Signal Transducer and Activator of Transcription 4 (STAT4) is a BOSTER BIO | ELISA KITS, ANTIBODIES, ANTIBODY COMPANYSIGN INDISTRIBUTORSABOUT USCONTACT USTISSUE/CELL TYPESTOTAL PROTEINANALYSIS
Boster Bio offers over 16,000 antibodies that have been validated for IHC, WB, ELISA, and FC in human, mouse, and rat samples. Rabbit and mouse monoclonal antibodies as well as rabbit polyclonal antibodies are available. More than 1,000 ELISA kits, both singleplex and multiplex, that have been cited 6,000+ times are available at Boster.IL34 ANTIBODY
100μg/vial. Storage & Handling. Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. Description. Boster Bio Anti-IL34 Antibody Picoband™ catalog # A06903-2. Tested in IHC-P, WBapplications.
WESTERN BLOT SAMPLE PREPARATION GUIDE Take out the protein solution in the middle and put it in a fresh tube. Mix the protein solution with Boster SDS-PAGE loading buffer 2X (Catalog# AR0131) in the same volume or with SDS-PAGE loading buffer 5X (Catalog# AR1112) in volume ratio of 4:1 evenly. Denature the solution in 100°C water bath for 5 minutes. ELISA BLOCKING OPTIMIZATION There are a variety of blocking buffers, not one of which is ideal for every combination of plate type, assay format, and detection system. Every blocking buffer represents a compromise between reducing background and maintaining specificity. Use this guide to help decide which type of blocking buffer is best suited for your specificapplication.
FLOW CYTOMETRY SAMPLE PREPARATION Transfer to a chilled centrifuge tube and add ice cold culture medium drop by drop until the cells are diluted 10X. Perform on ice! Centrifuge at 300-400 x g for 5 min at 4°C. Discard supernatant and wash once with cold staining buffer. Re-suspend cells in a suitable volume of cold staining buffer. MOUSE RAT TESTOSTERONE ELISA KIT Mouse Rat Testosterone ELISA Kit. Catalog#. EK7014. Storage & Handling. Store the kit at 2°C to 8°C. Keep microwells sealed in a dry bag with desiccants. The reagents are stable until expiration of the kit. Do not expose reagent to heat, sun, or strong light. Avoid multiple freeze-thaw cycles (Shipped with wet ice.) IMMUNOHISTOCHEMISTRY PRINCIPLE & PROTOCOL Immunohistochemistry (IHC) is a method for detecting antigens or haptens in cells of a tissue section by exploiting the principle of antibodies binding specifically to antigens in biological tissues. The antibody-antigen binding can be visualized in different manners. Enzymes, such as Horseradish Peroxidase (HRP) or Alkaline Phosphatase(AP
HOW TO DETERMINE ANTIBODY CROSS-REACTIVITYHOW TO DETERMINE REACTIVITYELEMENTS
We recommend an alignment score of over 85% as a good indication that an antibody may cross-react. For example, if the homology of the human sequence and rat sequence is higher than 90%, then, in theory, the antibody will cross-react with rat. The following image shows an example of what the results of a sequence alignment might look like. ANTI-SEPIAPTERIN REDUCTASE SPR ANTIBODY Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles. Description. Boster Bio Anti-Sepiapterin reductase SPR Antibody catalog # A00416. Tested in IHC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. Supplied as 100ul in Liquid formantibody.
SDS-PAGE OPTIMIZATION SDS-PAGE is the most widely used method for separating proteins by their relative molecular weights. By denaturing proteins in the presence of SDS, an ionic detergent, proteins can be linearized and imbued with a negative charge. This allows for an electric field to press them through the polyacrylamide gel matrix. BOSTER BIO | ELISA KITS, ANTIBODIES, ANTIBODY COMPANYSIGN INDISTRIBUTORSABOUT USCONTACT USTISSUE/CELL TYPESTOTAL PROTEINANALYSIS
Boster Bio offers over 16,000 antibodies that have been validated for IHC, WB, ELISA, and FC in human, mouse, and rat samples. Rabbit and mouse monoclonal antibodies as well as rabbit polyclonal antibodies are available. More than 1,000 ELISA kits, both singleplex and multiplex, that have been cited 6,000+ times are available at Boster.IL34 ANTIBODY
100μg/vial. Storage & Handling. Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. Description. Boster Bio Anti-IL34 Antibody Picoband™ catalog # A06903-2. Tested in IHC-P, WBapplications.
WESTERN BLOT SAMPLE PREPARATION GUIDE Take out the protein solution in the middle and put it in a fresh tube. Mix the protein solution with Boster SDS-PAGE loading buffer 2X (Catalog# AR0131) in the same volume or with SDS-PAGE loading buffer 5X (Catalog# AR1112) in volume ratio of 4:1 evenly. Denature the solution in 100°C water bath for 5 minutes. ELISA BLOCKING OPTIMIZATION There are a variety of blocking buffers, not one of which is ideal for every combination of plate type, assay format, and detection system. Every blocking buffer represents a compromise between reducing background and maintaining specificity. Use this guide to help decide which type of blocking buffer is best suited for your specificapplication.
FLOW CYTOMETRY SAMPLE PREPARATION Transfer to a chilled centrifuge tube and add ice cold culture medium drop by drop until the cells are diluted 10X. Perform on ice! Centrifuge at 300-400 x g for 5 min at 4°C. Discard supernatant and wash once with cold staining buffer. Re-suspend cells in a suitable volume of cold staining buffer. MOUSE RAT TESTOSTERONE ELISA KIT Mouse Rat Testosterone ELISA Kit. Catalog#. EK7014. Storage & Handling. Store the kit at 2°C to 8°C. Keep microwells sealed in a dry bag with desiccants. The reagents are stable until expiration of the kit. Do not expose reagent to heat, sun, or strong light. Avoid multiple freeze-thaw cycles (Shipped with wet ice.) IMMUNOHISTOCHEMISTRY PRINCIPLE & PROTOCOL Immunohistochemistry (IHC) is a method for detecting antigens or haptens in cells of a tissue section by exploiting the principle of antibodies binding specifically to antigens in biological tissues. The antibody-antigen binding can be visualized in different manners. Enzymes, such as Horseradish Peroxidase (HRP) or Alkaline Phosphatase(AP
HOW TO DETERMINE ANTIBODY CROSS-REACTIVITYHOW TO DETERMINE REACTIVITYELEMENTS
We recommend an alignment score of over 85% as a good indication that an antibody may cross-react. For example, if the homology of the human sequence and rat sequence is higher than 90%, then, in theory, the antibody will cross-react with rat. The following image shows an example of what the results of a sequence alignment might look like. ANTI-SEPIAPTERIN REDUCTASE SPR ANTIBODY Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles. Description. Boster Bio Anti-Sepiapterin reductase SPR Antibody catalog # A00416. Tested in IHC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. Supplied as 100ul in Liquid formantibody.
SDS-PAGE OPTIMIZATION SDS-PAGE is the most widely used method for separating proteins by their relative molecular weights. By denaturing proteins in the presence of SDS, an ionic detergent, proteins can be linearized and imbued with a negative charge. This allows for an electric field to press them through the polyacrylamide gel matrix. MOUSE RAT TESTOSTERONE ELISA KIT Mouse Rat Testosterone ELISA Kit. Catalog#. EK7014. Storage & Handling. Store the kit at 2°C to 8°C. Keep microwells sealed in a dry bag with desiccants. The reagents are stable until expiration of the kit. Do not expose reagent to heat, sun, or strong light. Avoid multiple freeze-thaw cycles (Shipped with wet ice.) ELISA FUNDAMENTAL PRINCIPLE, HOW IT WORKS ELISA (enzyme-linked immunosorbent assay) is a plate-based assay technique designed for detecting and quantifying peptides, proteins, antibodies, and hormones. In ELISA, an antigen must be immobilized to a solid surface and then complexed with an antibody that is linked toan enzyme.
ANTI-SYNCYTIN-2 ERVFRD-1 ANTIBODY Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles. Description. Boster Bio Anti-Syncytin-2 ERVFRD-1 Antibody catalog # A10341. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. Supplied as 100ul in Liquid form antibody.NUCLEAR FAST RED
Boster’s Nuclear Fast Red is an optimized nuclear counterstain solution that produces an intense nuclear stain. It is an anionic dye used in conjunction with an aluminium salt as a mordant. In contrast to hematoxylin, Nuclear Fast Red is a rapid staining procedure, whichrequires only
ANTI-MAP3KL4 ANTIBODY (A30507) For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles. Description. Boster Bio Anti-MAP3KL4 MAP3K21 Antibody catalog # A30507. Tested in WB applications. This antibody reacts with Human. Supplied as ANTI-USP19 ANTIBODY A05870 Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles. Description. Boster Bio Anti-USP19 Antibody catalog # A05870. Tested in IHC, WB applications. This antibody reacts with WESTERN BLOTTING PRINCIPLE Western Blotting (also called immunoblotting) is a technique used for analysis of individual proteins in a protein mixture (e.g. a cell lysate). The proteins on this immunoblot are then accessible for antibody binding for detection. Antibodies are used to detect target proteins on the western blot (immunoblot). Q4. DENTINOGENESIS RELATED GENES AND FACTS Quite a number of genes have been found to play important roles in Dentinogenesis, such as ALPP, ATRNL1, Asrgl1, BGLAP, CCL27, COL1A1, COL1A2, CXXC1, DMP1, DMTF1, DSP, DSPP, FN1, IBSP, PDLIM3, RUNX2, SLC4A1, SLPI, SPP1. See what Boster has to offer for the research of these genes by clicking the gene name links below and view a moredetailed
ELISA ANTIBODY CONCENTRATION OPTIMIZATION Antibody / Antigen. Concentration. In order to get the best results from your ELISA assay, the dilution factors of the sample and the detection antibodies must be optimized. For strong, quantifiable signal, use a checkerboard titration to test for the optimal concentration of sample and detection antibodies. STAT4 LUCIFERASE REPORTER-BA/F3 CELL LINE The STAT4 Luciferase Reporter cell line is a stably transfected Ba/F3 cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the STAT4 responsive promoter, so that the cell line is designed to measure the transcriptional activity of STAT4. Signal Transducer and Activator of Transcription 4 (STAT4) is a BOSTER BIO | ELISA KITS, ANTIBODIES, ANTIBODY COMPANYSIGN INDISTRIBUTORSABOUT USCONTACT USTISSUE/CELL TYPESTOTAL PROTEINANALYSIS
Boster Bio offers over 16,000 antibodies that have been validated for IHC, WB, ELISA, and FC in human, mouse, and rat samples. Rabbit and mouse monoclonal antibodies as well as rabbit polyclonal antibodies are available. More than 1,000 ELISA kits, both singleplex and multiplex, that have been cited 6,000+ times are available at Boster.IL34 ANTIBODY
100μg/vial. Storage & Handling. Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. Description. Boster Bio Anti-IL34 Antibody Picoband™ catalog # A06903-2. Tested in IHC-P, WBapplications.
WESTERN BLOT SAMPLE PREPARATION GUIDE Take out the protein solution in the middle and put it in a fresh tube. Mix the protein solution with Boster SDS-PAGE loading buffer 2X (Catalog# AR0131) in the same volume or with SDS-PAGE loading buffer 5X (Catalog# AR1112) in volume ratio of 4:1 evenly. Denature the solution in 100°C water bath for 5 minutes. ELISA BLOCKING OPTIMIZATION There are a variety of blocking buffers, not one of which is ideal for every combination of plate type, assay format, and detection system. Every blocking buffer represents a compromise between reducing background and maintaining specificity. Use this guide to help decide which type of blocking buffer is best suited for your specificapplication.
FLOW CYTOMETRY SAMPLE PREPARATION Transfer to a chilled centrifuge tube and add ice cold culture medium drop by drop until the cells are diluted 10X. Perform on ice! Centrifuge at 300-400 x g for 5 min at 4°C. Discard supernatant and wash once with cold staining buffer. Re-suspend cells in a suitable volume of cold staining buffer. MOUSE RAT TESTOSTERONE ELISA KIT Mouse Rat Testosterone ELISA Kit. Catalog#. EK7014. Storage & Handling. Store the kit at 2°C to 8°C. Keep microwells sealed in a dry bag with desiccants. The reagents are stable until expiration of the kit. Do not expose reagent to heat, sun, or strong light. Avoid multiple freeze-thaw cycles (Shipped with wet ice.) IMMUNOHISTOCHEMISTRY PRINCIPLE & PROTOCOL Immunohistochemistry (IHC) is a method for detecting antigens or haptens in cells of a tissue section by exploiting the principle of antibodies binding specifically to antigens in biological tissues. The antibody-antigen binding can be visualized in different manners. Enzymes, such as Horseradish Peroxidase (HRP) or Alkaline Phosphatase(AP
HOW TO DETERMINE ANTIBODY CROSS-REACTIVITY We recommend an alignment score of over 85% as a good indication that an antibody may cross-react. For example, if the homology of the human sequence and rat sequence is higher than 90%, then, in theory, the antibody will cross-react with rat. The following image shows an example of what the results of a sequence alignment might look like. ANTI-SEPIAPTERIN REDUCTASE SPR ANTIBODY Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles. Description. Boster Bio Anti-Sepiapterin reductase SPR Antibody catalog # A00416. Tested in IHC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. Supplied as 100ul in Liquid formantibody.
SDS-PAGE OPTIMIZATION SDS-PAGE is the most widely used method for separating proteins by their relative molecular weights. By denaturing proteins in the presence of SDS, an ionic detergent, proteins can be linearized and imbued with a negative charge. This allows for an electric field to press them through the polyacrylamide gel matrix. BOSTER BIO | ELISA KITS, ANTIBODIES, ANTIBODY COMPANYSIGN INDISTRIBUTORSABOUT USCONTACT USTISSUE/CELL TYPESTOTAL PROTEINANALYSIS
Boster Bio offers over 16,000 antibodies that have been validated for IHC, WB, ELISA, and FC in human, mouse, and rat samples. Rabbit and mouse monoclonal antibodies as well as rabbit polyclonal antibodies are available. More than 1,000 ELISA kits, both singleplex and multiplex, that have been cited 6,000+ times are available at Boster.IL34 ANTIBODY
100μg/vial. Storage & Handling. Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. Description. Boster Bio Anti-IL34 Antibody Picoband™ catalog # A06903-2. Tested in IHC-P, WBapplications.
WESTERN BLOT SAMPLE PREPARATION GUIDE Take out the protein solution in the middle and put it in a fresh tube. Mix the protein solution with Boster SDS-PAGE loading buffer 2X (Catalog# AR0131) in the same volume or with SDS-PAGE loading buffer 5X (Catalog# AR1112) in volume ratio of 4:1 evenly. Denature the solution in 100°C water bath for 5 minutes. ELISA BLOCKING OPTIMIZATION There are a variety of blocking buffers, not one of which is ideal for every combination of plate type, assay format, and detection system. Every blocking buffer represents a compromise between reducing background and maintaining specificity. Use this guide to help decide which type of blocking buffer is best suited for your specificapplication.
FLOW CYTOMETRY SAMPLE PREPARATION Transfer to a chilled centrifuge tube and add ice cold culture medium drop by drop until the cells are diluted 10X. Perform on ice! Centrifuge at 300-400 x g for 5 min at 4°C. Discard supernatant and wash once with cold staining buffer. Re-suspend cells in a suitable volume of cold staining buffer. MOUSE RAT TESTOSTERONE ELISA KIT Mouse Rat Testosterone ELISA Kit. Catalog#. EK7014. Storage & Handling. Store the kit at 2°C to 8°C. Keep microwells sealed in a dry bag with desiccants. The reagents are stable until expiration of the kit. Do not expose reagent to heat, sun, or strong light. Avoid multiple freeze-thaw cycles (Shipped with wet ice.) IMMUNOHISTOCHEMISTRY PRINCIPLE & PROTOCOL Immunohistochemistry (IHC) is a method for detecting antigens or haptens in cells of a tissue section by exploiting the principle of antibodies binding specifically to antigens in biological tissues. The antibody-antigen binding can be visualized in different manners. Enzymes, such as Horseradish Peroxidase (HRP) or Alkaline Phosphatase(AP
HOW TO DETERMINE ANTIBODY CROSS-REACTIVITYHOW TO DETERMINE REACTIVITYELEMENTS
We recommend an alignment score of over 85% as a good indication that an antibody may cross-react. For example, if the homology of the human sequence and rat sequence is higher than 90%, then, in theory, the antibody will cross-react with rat. The following image shows an example of what the results of a sequence alignment might look like. ANTI-SEPIAPTERIN REDUCTASE SPR ANTIBODY Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles. Description. Boster Bio Anti-Sepiapterin reductase SPR Antibody catalog # A00416. Tested in IHC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. Supplied as 100ul in Liquid formantibody.
SDS-PAGE OPTIMIZATION SDS-PAGE is the most widely used method for separating proteins by their relative molecular weights. By denaturing proteins in the presence of SDS, an ionic detergent, proteins can be linearized and imbued with a negative charge. This allows for an electric field to press them through the polyacrylamide gel matrix. MOUSE RAT TESTOSTERONE ELISA KIT Mouse Rat Testosterone ELISA Kit. Catalog#. EK7014. Storage & Handling. Store the kit at 2°C to 8°C. Keep microwells sealed in a dry bag with desiccants. The reagents are stable until expiration of the kit. Do not expose reagent to heat, sun, or strong light. Avoid multiple freeze-thaw cycles (Shipped with wet ice.) ELISA FUNDAMENTAL PRINCIPLE, HOW IT WORKS ELISA (enzyme-linked immunosorbent assay) is a plate-based assay technique designed for detecting and quantifying peptides, proteins, antibodies, and hormones. In ELISA, an antigen must be immobilized to a solid surface and then complexed with an antibody that is linked toan enzyme.
ANTI-SYNCYTIN-2 ERVFRD-1 ANTIBODY Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles. Description. Boster Bio Anti-Syncytin-2 ERVFRD-1 Antibody catalog # A10341. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. Supplied as 100ul in Liquid form antibody.NUCLEAR FAST RED
Boster’s Nuclear Fast Red is an optimized nuclear counterstain solution that produces an intense nuclear stain. It is an anionic dye used in conjunction with an aluminium salt as a mordant. In contrast to hematoxylin, Nuclear Fast Red is a rapid staining procedure, whichrequires only
ANTI-MAP3KL4 ANTIBODY (A30507) For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles. Description. Boster Bio Anti-MAP3KL4 MAP3K21 Antibody catalog # A30507. Tested in WB applications. This antibody reacts with Human. Supplied as ANTI-USP19 ANTIBODY A05870 Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles. Description. Boster Bio Anti-USP19 Antibody catalog # A05870. Tested in IHC, WB applications. This antibody reacts with WESTERN BLOTTING PRINCIPLE Western Blotting (also called immunoblotting) is a technique used for analysis of individual proteins in a protein mixture (e.g. a cell lysate). The proteins on this immunoblot are then accessible for antibody binding for detection. Antibodies are used to detect target proteins on the western blot (immunoblot). Q4. DENTINOGENESIS RELATED GENES AND FACTS Quite a number of genes have been found to play important roles in Dentinogenesis, such as ALPP, ATRNL1, Asrgl1, BGLAP, CCL27, COL1A1, COL1A2, CXXC1, DMP1, DMTF1, DSP, DSPP, FN1, IBSP, PDLIM3, RUNX2, SLC4A1, SLPI, SPP1. See what Boster has to offer for the research of these genes by clicking the gene name links below and view a moredetailed
ELISA ANTIBODY CONCENTRATION OPTIMIZATION Antibody / Antigen. Concentration. In order to get the best results from your ELISA assay, the dilution factors of the sample and the detection antibodies must be optimized. For strong, quantifiable signal, use a checkerboard titration to test for the optimal concentration of sample and detection antibodies. STAT4 LUCIFERASE REPORTER-BA/F3 CELL LINE The STAT4 Luciferase Reporter cell line is a stably transfected Ba/F3 cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the STAT4 responsive promoter, so that the cell line is designed to measure the transcriptional activity of STAT4. Signal Transducer and Activator of Transcription 4 (STAT4) is a JAVASCRIPT SEEMS TO BE DISABLED IN YOUR BROWSER. For the best experience on our site, be sure to turn on Javascript in your browser. THIS WEBSITE USES COOKIES TO ENSURE YOU GET THE BEST EXPERIENCE ON OURWEBSITE.
Got it!
Skip to Content
* Compare Products
(NOT SET)
__
* __
__ * __
__Sign In
*
__
* __
* __ Home __
* PRODUCTS __
ALL PRODUCT TYPES
* primary antibodies __ * Monoclonal Antibodies * Rabbit Monoclonal Antibodies* ELISA kits __
* PicoKineâ„¢ ELISA Kits * PicoKineâ„¢ Quick ELISA Kits90-min protocol * EZ Setâ„¢ ELISA Antibody Pairs * Multiplex ELISA Solutionsonly 25ul of sample! * Secondary AntibodiesFEATURED PRODUCTS
* Recombinant Proteins * Common Buffers And Recipes * Cell and Tissue Lysates * Immunostaining IHC IF ICC Reagents * Western Blot Reagents* PCR and qPCR
* Reporter Cell Lines * Instruments and Machines __ * qPCR Thermo Cycler real time PCR machineBY RESEARCH AREAS
* COVID-19 Products & ServicesBY DISEASES
* Browse All DiseasesBY PATHWAYS
* Browse all pathways GENES INFOGRAPHICS A-Z* A
* b
* c
* d
* e
* f
* g
* h
* i
* j
* k
* l
* m
* n
* o
* p
* q
* r
* s
* t
* u
* v
* w
* x
* y
* z
* SERVICES __
ALL SERVICES
* CUSTOM PRODUCTIONS*
* Custom Antibody Production__* Rabbit Polyclonal
* Special Deals For Custom Antibody* Mouse Monoclonal
* Rabbit Monoclonal
* Recombinant Protein Expression * Gene Editing Services * All CRO Assay Services * ELISA Testing Service * IF/ICC and IHC Services * Western Blotting Services * Antibody Validation Services * INSTRUCTIONS AND GUIDELINES * Sample Collection Guidelines * TECHNICAL RESOURCES __ ALL TECHNICAL RESOURCES * WESTERN BLOT RESOURCE CENTER * Western Blotting Principle * Western Blot Glossary * Western Blotting Protocol * Western Blotting Troubleshooting Tips * Western Blotting Optimization Tips * IHC/ICC/IF RESOURCE CENTER * IHC/ICC/IF Principle * Immunofluorescence Glossary * IHC/ICC/IF Sample Preparation * IHC/ICC/IF Protocol * IHC/ICC/IF Troubleshooting Tips * IHC/ICC/IF Optimization Tips * ELISA RESOURCE CENTER* ELISA Principle
* ELISA Sample Preparation* ELISA Protocol
* ELISA Troubleshooting Tips * ELISA Optimization Tips * ELISA Data Analysis * FLOW CYTOMETRY RESOURCE CENTER * Flow Cytometry Principle * Flow Cytometry Sample Preparation * Flow Cytometry Protocol * Flow Cytometry Troubleshooting Tips * Flow Cytometry Optimization Tips * PCR RESOURCE CENTER* PCR Principle
* PCR Sample Preparation* PCR Protocols
* PCR Troubleshooting Tips * EDUCATIONAL RESOURCES* Pathway Maps
* Gene Info Cards
* BOSTER'S EXCLUSIVE SCIENCE TIPS * Expert Tips on IHC Optimization * The Savvy Scientists' Buffers Guide * __ Biomarker Research via EXCEL TROUBLESHOOTING EBOOKSDownload Now
* PROMOTIONS __
FEATURED PROMOTIONS
* Buy Primary Get Secondary Free * Deep Discount Custom Antibody Production View All Promotions * DISTRIBUTORS__* About us __
* About us
* Antibody Company
* Boster Guarantee
* Antibodies Validation* ELISA Validation
* Testimonials
* Terms and Conditions * Newsletter Archive * Career Opportunities* Privacy Policy
* Terms of Service
* Ordering FAQs
* Scholarships
* CONTACT US
__
* Table of Content
* __
* __
* __
ANTIBODY & ELISA EXPERTS 27 years designing high affinity antibodies and immunoassays. Proudly serving the brightest minds in scientific research.__
FEATURED PRODUCTS & SERVICES PICOKINEâ„¢ QUICK ELISA KITS ELISA RESULTS IN 90 MINUTES We are excited to launch our new product line - PicoKineâ„¢ Quick ELISA Kits! While traditional ELISA kits take 3+ hours to run, our Quick ELISA kits only need 90 minutes to generate publishable and reproducible results. The sensitive Quick ELISA kits require shorter incubation times and less sample to perform, so you can save time and sample without sacrificing quality.Check them out
CUSTOM ANTIBODIES FOR ONLY $600 MOST AFFORDABLE CUSTOM ANTIBODY PROGRAM Our team has worked with customers like you on hundreds of projects to develop custom antibodies for various research applications. We offer a $600 promotion for custom antibody projects in the categories below: * You work with non-traditional model organisms (i.e. not human,mouse, rat).
* Your work is related to COVID-19. * You want to replace a discontinued antibody (from any company). See if my project qualifies OUTSOURCE YOUR IHC, WB, ELISA EXPERIMENTS ACCELERATE YOUR RESEARCH AND PUBLISH MORE Falling behind on your projects? Want to save budget? Outsource your ELISA, WB, and IHC experiments using Boster Bio's affordable and high quality CRO services. With our services, we do the work for you and generate publishable data, so you can focus on your core researchactivities.
Find out more
16,000+ ANTIBODIES AVAILABLE VALIDATED FOR IHC, WB, ELISA, FLOW CYTOMETRY Since 1993, Boster has provided scientists worldwide with superior antibodies and ELISA kits. We currently offer more than 16,000 antibodies and 2,000 ELISA kits that have received over 29,000 citations. Our lab monitors in-house manufacturing and rigorously validates our antibodies, ELISA kits, and reagents to ensure qualityand consistency.
Browse all antibodies__ __
PRODUCT CATEGORIES
* Primary Antibodies* ELISA Kits
* Recombinant Proteins * Secondary Antibodies* Reagents Kits
* Primary Antibodies* ELISA Kits
* Recombinant Proteins * Secondary Antibodies* Reagents Kits
PRIMARY ANTIBODY
Boster Bio offers over 16,000 antibodies that have been validated for IHC, WB, ELISA, and FC in human, mouse, and rat samples. Rabbit and mouse monoclonal antibodies as well as rabbit polyclonal antibodiesare available.
* All Primary Antibodies * Monoclonal Antibodies * Rabbit Monoclonal AntibodiesELISA KITS
More than 1,000 ELISA kits, both singleplex and multiplex, that have been cited 6,000+ times are available at Boster. We offer our Boster-branded Picokineâ„¢ ELISA kits (sandwich ELISA) and EZ-Setâ„¢ ELISA kits (DIY antibody pairs) in addition to many multiplex ELISAkits.
* All ELISA Kits
* Picokineâ„¢ ELISA Kits * Picokineâ„¢ Fast ELISA Kits * EZ-Setâ„¢ ELISA Kits * Multiplex ELISA Solution RECOMBINANT PROTEINS Boster provides a wide range of human, mouse, and rat recombinant proteins, which include cytokines, growth factors, chemokines, and many more. Our recombinant proteins have high stability, biological activity, and purity. * All Recombinant Proteins * Murine Recombinant Proteins * Human Recombinant Proteins SECONDARY ANTIBODIES Secondary antibodies for different conjugations (HRP, biotin, FITC, DyLight, etc.) are available for your research. Our SABC and Super Vision detection kits provide specific secondary antibodies along with all the reagents needed to perform IHC and ICC. * All Secondary Antibodies * SABC Detection Kits * Super Vision Detection KitsREAGENTS
Boster offers all the reagents you need for your immunostaining and western blotting experiments. We've got everything from buffers to lysates to kits, including our popular and well-cited BCA, CCK-8, andMTT assay kits.
* Immunostaining Reagents* WB Reagents
* Purification Beads * Cell Lines and Lysates * Total Protein Analysis Kits * Cell Proliferation & Cytotoxicity * Buffers (RIPA, PBS, PFA, etc.) * Enzyme Activity Assay KitsSERVICES
Mouse over one of the services below to see more infomationPRIMARY ANTIBODY
Custom antibody services include antigen design and expression and antigen purification for both polyclonal and monoclonal. Competitivelypriced.
PROTEIN EXPRESSION
Custom recombinant protein expression services cover feasibility assessment and scale up production, in E. Coli, and insert/mamaliancell lines.
ELISA SERVICES
Boster Bio will test your samples and provide you scientific consultation, backed by our network of experts with experience in youranalytes.
WESTERN BLOT SERVICES Boster offers competitively priced Western blot services with quick turnaround. We also provide 2D-WB and LC-MS for advancedcharacterization.
IHC/IF SERVICES
Boster offers competitively priced histology/IHC/IF services with quick turnaround. Featuring a large selection of FFPE blocks andtissue/cell arrays.
ANTIBODY VALIDATION
Need to validate an antibody? We can validate it for you. We will purchase the antibody on your behalf and test it on WB & IHC or customassays.
ANTIBODY CONJUGATION Want your antibody conjugated? Boster offers a large selection of conjugates and offers a competitively priced, worry-free conjugationservice.
__ __
*
PRIMARY ANTIBODY
PRIMARY ANTIBODY
Custom antibody services include antigen design and expression and antigen purification for both polyclonal and monoclonal. Competitivelypriced.
*
PROTEIN EXPRESSION
PROTEIN EXPRESSION
Custom recombinant protein expression services cover feasibility assessment and scale up production, in E. Coli, and insert/mamaliancell lines.
*
ELISA SERVICES
ELISA SERVICES
Boster Bio will test your samples and provide you scientific consultation, backed by our network of experts with experience in youranalytes.
*
WESTERN BLOT SERVICESWB SERVICES
Boster offers competitively priced Western blot services with quick turnaround. We also provide 2D-WB and LC-MS for advancedcharacterization.
*
IHC/IF SERVICES
IHC/IF SERVICES
Boster offers competitively priced histology/IHC/IF services with quick turnaround. Featuring a large selection of FFPE blocks andtissue/cell arrays.
*
ANTIBODY VALIDATION
ANTIBODY VALIDATION
Need to validate an antibody? We can validate it for you. We will purchase the antibody on your behalf and test it on WB & IHC or customassays.
*
ANTIBODY CONJUGATION ANTIBODY CONJUGATION Want your antibody conjugated? Boster offers a large selection of conjugates and offers a competitively priced, worry-free conjugationservice.
TECHNICAL RESOURCES
* IHC
* Western Blotting
* ELISA Kits
* Flow Cytometry
* Polymer Chain Reaction (PCR)* Ebooks
WELCOME TO DR. BOOSTER'S LIFE SCIENCE LAB Welcome to my lab! Let me show you around. Click one of the experiments on the table to see more details.*
IHC
__
*
WB
__
*
ELISA
__
*
FC
__
*
PCR
__
*
Ebooks
__
ABOUT BOSTER BIOLOGICAL TECHNOLOGY27
Years
29000+
Publications
4.8 / 5
Rating on biocompare.com16000+
Antibodies
2000+
ELISA Kits
TESTIMONIALS
__ __
MARIA TERESA DELL'ANNOPostdoc
* __
* __
* __
* __
* __
This Antibody Works Perfectly!APPLICATION
ELISA
STARTING MATERIAL
Crispr MEK and STAT3 KO CAFsTIPS
This kit doesn't provide PC I used it for IHC on frozen sections at a dilution of 1:500. It did not need several trials to optimize the protocol. No bad things overall. I will purchase it again.__ __
LU YANG
Graduate Student
* __
* __
* __
* __
* __
Competitively Priced And WorkedAPPLICATIONS
Protein level screenSAMPLE
Cell culture media from MCF7 cellsDETECTION
--
The TFF1 ELISA kit from Boster was competitively priced and worked with cell culture media taken from MCF7 cells, a human mammary carcinoma which is well known for overexpression of the TFF1 protein. The kit is very sensitive, detecting TFF1 protein at levels of 10pg/mL. The kit is saturated about 1mg/mL, so in my case, a 1 to 100 dilution of cell culture media was needed in order to be in the linear range of the assay. Good ELISA kit for human TFF1 protein. Worked asadvertised.
__ __
RUBY C
Research Assistant
* __
* __
* __
* __
* __
BosterBio Sparcl1 WorksAPPLICATIONS
ImmunohistochemistrySAMPLE
Mouse astrocytes
DETECTION
Confocal/Fluorescent Scope The general aim of the experiment was to determine molecular differences of astrocytes in the central nervous system. We wanted to see if there are different expression levels of Sparcl1 in different regions of the mouse brain as a way to compare and possibly validate qPCR data. The product was found on One World Lab and it worked so we decided to buy a greater quantity as opposed to a trial size. Thebottom line: Good.
__ __
View All Testimonials PARTNERS & CUSTOMERSPRODUCTS
* All Categories
* Primary Antibodies * PicoKineâ„¢ ELISA Kits * Recombinant Proteins * Secondary Antibodies * Immunoblotting Reagents* IHC/IF Reagents
PRODUCTS BY
* A-Z Genes
* Diseases
* Pathways
* __
* __
* __
SERVICES
* All Services
* Recombinant Protein Expression* Gene Synthesis
* Custom Antibody Production * --Rabbit Polyclonal * --Mouse Monoclonal * --Rabbit Monoclonal * Sample Testing Services * --Singleplex ELISA* --Multiplex ELISA
* --IHC and Immunofluorescence* Assay Services
* --ELISA
* --Histology/IHC/IF * --Western BlottingTECHNICAL RESOURCES
* All Support Contents * ELISA Technical Resource Center * Western Blot Resource Center * IHC/ICC Resource Center * Flow Cytometry Resource Center* ChIP eBook
POPULAR PRODUCTS
* TNF Alpha ELISA Kit* IL6 ELISA Kit
* VEGF ELISA Kit
* 4% Paraformaldehyde* PD-L1 Antibody
ABOUT US
* Contact Us
* Distributors
* Career OpportunitiesCONTACT US
* __ Tel: (888)466-3604 * __ Fax: (925)215-2184 * __ Order: orders@bosterbio.com * __ Sales: sales@bosterbio.com * __ Support: support@bosterbio.com * __ Business: boster@bosterbio.comADDRESS
* Boster Biological Technology* 3942 Valley Ave
* Pleasanton, CA 94566, USA* __
© 1993-2021 Boster Biological Technology. All rights reserved.* __
* __
* __
Details
Copyright © 2024 ArchiveBay.com. All rights reserved. Terms of Use | Privacy Policy | DMCA | 2021 | Feedback | Advertising | RSS 2.0