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PCR TROUBLESHOOTING
Increase primer amount. Increase template amount. Decrease annealing temperature by 6-10º C and check if you get any product. If you don't, check all your PCR ingredients. If you do get products (including unspecific ones) reaction conditions as described above. Combine some/all of the above. 4. PHOTOINITIATORS FOR UVCURING 200 220 240 260 280 300 320 340 1.2 1.0 0.8 0.6 0.4 0.2 0.0 Key products, surface cure Absorption Spectra 0.001% (Acetonitrile) Wavelength (nm) Absorbance IRGACURE 184 DAROCUR 1173 IRGACURE 754 IRGACURE 2959 200 220 240 260 280 300 320 340 360 380 THE COMMERCE CONTROL LIST Commerce Control List Supplement No. 1 to Part 774 Category 0—page 2 Export Administration Regulations Bureau of Industry and SecurityJanuary 6, 2014
THMR-IP5680 HP I-LINE PHOTORESIST 4 26-WE4C03-00 OHKA AMERICA, INC. 300 400 500 600 700 800 900 300 400 500 600 700 800 900 Mask CD (nm) Print CD (nm) THMR-iP5680 HP Target CD = 0.6 µm, exposure dose = 160 ms ADHESION ASSAY PROTOCOL Adhesion Assay Protocol: Materials to be prepared beforehand: 1) Washing Buffer--0.1% BSA in medium (DMEM or RPMI) 2) Blocking Buffer--0.5% BSA in medium (DMEM or RPMI) A LOW-COST, OPEN-SOURCE DIY DNA SYNTHESIZER PROJECT Building low-cost DNA synthesizers Our project has multiple phases, but the most interesting phase is the microfluidic chip. Microscopic channels guide the direction of fluids and reagents. Version 1: phosphoramidite chemistry. Version “the next one”: ligation chemistry from on- chip 4,096 droplet library (synthesis by shortoligo
GROWTH AND CHARACTERIZATION OF (110) INAS QUANTUM WELL Growth and Characterization of (110) InAs Quantum Well Heterostructures by Transmission Electron Microscopy and Electron Channeling Contrast Imaging. ADVANTAGES OF BEAM DECELERATION FOR LOW KV EDS ANALYSIS. Figure 1. EDS map of Slag at 3kV and 90nA: (a,c,e) No Gentle Beam; (b,d,f) -5kV Gentle Beam. a b e f c d a Microsc. Microanal. 21 (Suppl3), 2015 682
HIGH SPEED LOGIC CIRCUITS DR. LYNN FULLER November 5, 2014 Dr. Lynn Fuller High Speed Logic Page 3 Rochester Institute of Technology Microelectronic Engineering OUTLINEIntroduction
BASIC LABORATORY PRACTICES Basic Laboratory Practices. General Method for Cleavage of His-Tagged Proteins with Thrombin cleavage sites . Thrombin recognises the consensus sequence Leu-Val-Pro-Arg-Gly-Ser, cleaving the peptide bond between Arg and Gly. This is utilised in many vector systems which encode such a protease cleavage site allowing removal of an upstreamdomain.
PCR TROUBLESHOOTING
Increase primer amount. Increase template amount. Decrease annealing temperature by 6-10º C and check if you get any product. If you don't, check all your PCR ingredients. If you do get products (including unspecific ones) reaction conditions as described above. Combine some/all of the above. 4. PHOTOINITIATORS FOR UVCURING 200 220 240 260 280 300 320 340 1.2 1.0 0.8 0.6 0.4 0.2 0.0 Key products, surface cure Absorption Spectra 0.001% (Acetonitrile) Wavelength (nm) Absorbance IRGACURE 184 DAROCUR 1173 IRGACURE 754 IRGACURE 2959 200 220 240 260 280 300 320 340 360 380 THE COMMERCE CONTROL LIST Commerce Control List Supplement No. 1 to Part 774 Category 0—page 2 Export Administration Regulations Bureau of Industry and SecurityJanuary 6, 2014
THMR-IP5680 HP I-LINE PHOTORESIST 4 26-WE4C03-00 OHKA AMERICA, INC. 300 400 500 600 700 800 900 300 400 500 600 700 800 900 Mask CD (nm) Print CD (nm) THMR-iP5680 HP Target CD = 0.6 µm, exposure dose = 160 ms ADHESION ASSAY PROTOCOL Adhesion Assay Protocol: Materials to be prepared beforehand: 1) Washing Buffer--0.1% BSA in medium (DMEM or RPMI) 2) Blocking Buffer--0.5% BSA in medium (DMEM or RPMI) A LOW-COST, OPEN-SOURCE DIY DNA SYNTHESIZER PROJECT Building low-cost DNA synthesizers Our project has multiple phases, but the most interesting phase is the microfluidic chip. Microscopic channels guide the direction of fluids and reagents. Version 1: phosphoramidite chemistry. Version “the next one”: ligation chemistry from on- chip 4,096 droplet library (synthesis by shortoligo
GROWTH AND CHARACTERIZATION OF (110) INAS QUANTUM WELL Growth and Characterization of (110) InAs Quantum Well Heterostructures by Transmission Electron Microscopy and Electron Channeling Contrast Imaging. ADVANTAGES OF BEAM DECELERATION FOR LOW KV EDS ANALYSIS. Figure 1. EDS map of Slag at 3kV and 90nA: (a,c,e) No Gentle Beam; (b,d,f) -5kV Gentle Beam. a b e f c d a Microsc. Microanal. 21 (Suppl3), 2015 682
HIGH SPEED LOGIC CIRCUITS DR. LYNN FULLER November 5, 2014 Dr. Lynn Fuller High Speed Logic Page 3 Rochester Institute of Technology Microelectronic Engineering OUTLINEIntroduction
PCR TROUBLESHOOTING
Increase primer amount. Increase template amount. Decrease annealing temperature by 6-10º C and check if you get any product. If you don't, check all your PCR ingredients. If you do get products (including unspecific ones) reaction conditions as described above. Combine some/all of the above. 4. EDUCATION GUIDE SPECIAL STAINS 1 Introduction to Special Stains Sonja Wulff Thousands of years ago, diagnosing and treating a patient’s condition was much simpler. Ancient Egyptians blamed disease on demons, Greeks and Romans pointed XJ128 GUIDE TO OPERATION XJ128 and XJ128 Plus Printhead XJ128 Guide to Operation Xaar Document no: D031010302 Version A Page 6 1 Introduction 1.1 Overview This document is only valid for the Xaar XJ128 product. PROTEIN DETERMINATION BY THE BIURET METHOD Prepare a reference tube with 1 ml buffer. If possible, dilute unknowns to an estimated 1 to 10 mg/ml with buffer; a range of dilutions should be used if the actual concentration cannot be estimated. Use 1 ml sample per assay tube. Add 9 ml Biuret reagent to each tube, vortex immediately, and POLYETHYLENE GLYCOL-COATED BIOCOMPATIBLE SURFACES Polyethylene glycol–coated biocompatible surfaces Norma A. Alcantar, Eray S. Aydil, Jacob N. Israelachvili Chemical Engineering Department and Materials Department, University of California, Santa Barbara,California 93106
EISENLAB - DIYHPL
Maple Tree is a java-based, open source, cross-platform visualization tool to graphically browse the results of clustering analyses from our Cluster and Fuzzy K clustering software, and many other clustering and analysis programs. Maple Tree was developed by Lisa Simirenko in our lab. Combined Expression Data and Sequence Analysis. A LOW-COST, OPEN-SOURCE DIY DNA SYNTHESIZER PROJECT Building low-cost DNA synthesizers Our project has multiple phases, but the most interesting phase is the microfluidic chip. Microscopic channels guide the direction of fluids and reagents. Version 1: phosphoramidite chemistry. Version “the next one”: ligation chemistry from on- chip 4,096 droplet library (synthesis by shortoligo
AUTORADIOGRAPHY AND FLUOROGRAPHY Autoradiography is the direct exposure of film by beta particles or gamma rays. Fluorography is the exposure of film by secondary light that was generated by the excitation of a fluor or a screen by a beta particle or a gamma ray. Fluorography works best with flashed film and exposure of the film at - 70°C. CLINICAL DEVELOPMENT SUCCESS RATES FOR INVESTIGATIONAL DRUGS 42 volume 32 NumBeR 1 JANuARY 2014 nature biotechnology data to annotate drugs by their biochemi-cal composition (e.g., peptide, nucleic acid, monoclonal antibody (mAb)) and molecu-lar size (i.e., large and small molecules). A PATHETICALLY SIMPLE CHARGE SENSITIVE AMPLIFIER A Pathetically Simple Charge Sensitive Amplifier The schematic above shows an extremely simple charge sensitive amplifier with only twoactive components.
BASIC LABORATORY PRACTICES Basic Laboratory Practices. General Method for Cleavage of His-Tagged Proteins with Thrombin cleavage sites . Thrombin recognises the consensus sequence Leu-Val-Pro-Arg-Gly-Ser, cleaving the peptide bond between Arg and Gly. This is utilised in many vector systems which encode such a protease cleavage site allowing removal of an upstreamdomain.
PLANT ELECTROPHYSIOLOGY Preface Plant electrophysiology is the study of the electrochemical phenomena associated with biological cells and tissues in plants. It involves measurements of electrical PHOTOINITIATORS FOR UVCURING 200 220 240 260 280 300 320 340 1.2 1.0 0.8 0.6 0.4 0.2 0.0 Key products, surface cure Absorption Spectra 0.001% (Acetonitrile) Wavelength (nm) Absorbance IRGACURE 184 DAROCUR 1173 IRGACURE 754 IRGACURE 2959 200 220 240 260 280 300 320 340 360 380 POLYETHYLENE GLYCOL-COATED BIOCOMPATIBLE SURFACES Polyethylene glycol–coated biocompatible surfaces Norma A. Alcantar, Eray S. Aydil, Jacob N. Israelachvili Chemical Engineering Department and Materials Department, University of California, Santa Barbara,California 93106
ADHESION ASSAY PROTOCOL Adhesion Assay Protocol: Materials to be prepared beforehand: 1) Washing Buffer--0.1% BSA in medium (DMEM or RPMI) 2) Blocking Buffer--0.5% BSA in medium (DMEM or RPMI) YEAST CELL CYCLE FLOW CYTOMETRY PROTOCOL Protocol. Spin down 10 7 cells from an exponentially growing culture - 2000 rpm for 5 mins. Pour off supernatant. Vortex tube while adding 1.0 ml cold 70% EtOH. Store at 4 °C (cells keep ~indefinitely). When you want to process the cells, take 0.3 ml (this will be 2-3 x 10 6 cells, assuming a little loss in the washing) and add to 3 ml 50 mM EDUCATION GUIDE SPECIAL STAINS 1 Introduction to Special Stains Sonja Wulff Thousands of years ago, diagnosing and treating a patient’s condition was much simpler. Ancient Egyptians blamed disease on demons, Greeks and Romans pointed THMR-IP5680 HP I-LINE PHOTORESIST 4 26-WE4C03-00 OHKA AMERICA, INC. 300 400 500 600 700 800 900 300 400 500 600 700 800 900 Mask CD (nm) Print CD (nm) THMR-iP5680 HP Target CD = 0.6 µm, exposure dose = 160 ms CLINICAL DEVELOPMENT SUCCESS RATES FOR INVESTIGATIONAL DRUGS 42 volume 32 NumBeR 1 JANuARY 2014 nature biotechnology data to annotate drugs by their biochemi-cal composition (e.g., peptide, nucleic acid, monoclonal antibody (mAb)) and molecu-lar size (i.e., large and small molecules). LITHOGRAPHY USING ASML STEPPER Page 6 © April 28, 2014 Dr. Lynn Fuller Lithography Using ASML Stepper Page 11 CRITICAL MODULATION FOR RESIST (CMR) Critical Modulation Resist Gamma, γ 0 2 4 6 8 10 0 BASIC LABORATORY PRACTICES Basic Laboratory Practices. General Method for Cleavage of His-Tagged Proteins with Thrombin cleavage sites . Thrombin recognises the consensus sequence Leu-Val-Pro-Arg-Gly-Ser, cleaving the peptide bond between Arg and Gly. This is utilised in many vector systems which encode such a protease cleavage site allowing removal of an upstreamdomain.
PLANT ELECTROPHYSIOLOGY Preface Plant electrophysiology is the study of the electrochemical phenomena associated with biological cells and tissues in plants. It involves measurements of electrical PHOTOINITIATORS FOR UVCURING 200 220 240 260 280 300 320 340 1.2 1.0 0.8 0.6 0.4 0.2 0.0 Key products, surface cure Absorption Spectra 0.001% (Acetonitrile) Wavelength (nm) Absorbance IRGACURE 184 DAROCUR 1173 IRGACURE 754 IRGACURE 2959 200 220 240 260 280 300 320 340 360 380 POLYETHYLENE GLYCOL-COATED BIOCOMPATIBLE SURFACES Polyethylene glycol–coated biocompatible surfaces Norma A. Alcantar, Eray S. Aydil, Jacob N. Israelachvili Chemical Engineering Department and Materials Department, University of California, Santa Barbara,California 93106
ADHESION ASSAY PROTOCOL Adhesion Assay Protocol: Materials to be prepared beforehand: 1) Washing Buffer--0.1% BSA in medium (DMEM or RPMI) 2) Blocking Buffer--0.5% BSA in medium (DMEM or RPMI) YEAST CELL CYCLE FLOW CYTOMETRY PROTOCOL Protocol. Spin down 10 7 cells from an exponentially growing culture - 2000 rpm for 5 mins. Pour off supernatant. Vortex tube while adding 1.0 ml cold 70% EtOH. Store at 4 °C (cells keep ~indefinitely). When you want to process the cells, take 0.3 ml (this will be 2-3 x 10 6 cells, assuming a little loss in the washing) and add to 3 ml 50 mM EDUCATION GUIDE SPECIAL STAINS 1 Introduction to Special Stains Sonja Wulff Thousands of years ago, diagnosing and treating a patient’s condition was much simpler. Ancient Egyptians blamed disease on demons, Greeks and Romans pointed THMR-IP5680 HP I-LINE PHOTORESIST 4 26-WE4C03-00 OHKA AMERICA, INC. 300 400 500 600 700 800 900 300 400 500 600 700 800 900 Mask CD (nm) Print CD (nm) THMR-iP5680 HP Target CD = 0.6 µm, exposure dose = 160 ms CLINICAL DEVELOPMENT SUCCESS RATES FOR INVESTIGATIONAL DRUGS 42 volume 32 NumBeR 1 JANuARY 2014 nature biotechnology data to annotate drugs by their biochemi-cal composition (e.g., peptide, nucleic acid, monoclonal antibody (mAb)) and molecu-lar size (i.e., large and small molecules). LITHOGRAPHY USING ASML STEPPER Page 6 © April 28, 2014 Dr. Lynn Fuller Lithography Using ASML Stepper Page 11 CRITICAL MODULATION FOR RESIST (CMR) Critical Modulation Resist Gamma, γ 0 2 4 6 8 10 0PCR TROUBLESHOOTING
Increase primer amount. Increase template amount. Decrease annealing temperature by 6-10º C and check if you get any product. If you don't, check all your PCR ingredients. If you do get products (including unspecific ones) reaction conditions as described above. Combine some/all of the above. 4. PROTEIN DETERMINATION BY THE BIURET METHOD Prepare a reference tube with 1 ml buffer. If possible, dilute unknowns to an estimated 1 to 10 mg/ml with buffer; a range of dilutions should be used if the actual concentration cannot be estimated. Use 1 ml sample per assay tube. Add 9 ml Biuret reagent to each tube, vortex immediately, and EDUCATION GUIDE SPECIAL STAINS 1 Introduction to Special Stains Sonja Wulff Thousands of years ago, diagnosing and treating a patient’s condition was much simpler. Ancient Egyptians blamed disease on demons, Greeks and Romans pointed YEAST CELL CYCLE FLOW CYTOMETRY PROTOCOL Protocol. Spin down 10 7 cells from an exponentially growing culture - 2000 rpm for 5 mins. Pour off supernatant. Vortex tube while adding 1.0 ml cold 70% EtOH. Store at 4 °C (cells keep ~indefinitely). When you want to process the cells, take 0.3 ml (this will be 2-3 x 10 6 cells, assuming a little loss in the washing) and add to 3 ml 50 mM THMR-IP5680 HP I-LINE PHOTORESIST 4 26-WE4C03-00 OHKA AMERICA, INC. 300 400 500 600 700 800 900 300 400 500 600 700 800 900 Mask CD (nm) Print CD (nm) THMR-iP5680 HP Target CD = 0.6 µm, exposure dose = 160 msPINY-COMMANDS
CGI forms for Piny: root: summary refs log tree commit diff PERSONALITY AND SOCIAL PSYCHOLOGY REVIEW HTTP://PSR 2. Personality and Social Psychology Review XX(X) Nisbett et al., 2012b). Religion, on the other hand, has a more . intermittent history. Gorsuch (1988) noted that interest in the LITHOGRAPHY USING ASML STEPPER Page 6 © April 28, 2014 Dr. Lynn Fuller Lithography Using ASML Stepper Page 11 CRITICAL MODULATION FOR RESIST (CMR) Critical Modulation Resist Gamma, γ 0 2 4 6 8 10 0 CMOS TESTING FOR THE STUDENT RUN FACTORY CMOS Testing GENERAL TEST INSTRUCTIONS Test die in the center of the wafer, then upper left, upper right, lower right, and lower left (about ½ way between center and edge). FREQUENCY RESPONSE OF THE CE AMPLIFIER September 25, 2014 Dr. Lynn Fuller, Professor Rochester Institute of Technology Microelectronic Engineering Frequency Response Page 3INTRODUCTION
BASIC LABORATORY PRACTICES Basic Laboratory Practices. General Method for Cleavage of His-Tagged Proteins with Thrombin cleavage sites . Thrombin recognises the consensus sequence Leu-Val-Pro-Arg-Gly-Ser, cleaving the peptide bond between Arg and Gly. This is utilised in many vector systems which encode such a protease cleavage site allowing removal of an upstreamdomain.
PLANT ELECTROPHYSIOLOGY Preface Plant electrophysiology is the study of the electrochemical phenomena associated with biological cells and tissues in plants. It involves measurements of electrical PHOTOINITIATORS FOR UVCURING 200 220 240 260 280 300 320 340 1.2 1.0 0.8 0.6 0.4 0.2 0.0 Key products, surface cure Absorption Spectra 0.001% (Acetonitrile) Wavelength (nm) Absorbance IRGACURE 184 DAROCUR 1173 IRGACURE 754 IRGACURE 2959 200 220 240 260 280 300 320 340 360 380 POLYETHYLENE GLYCOL-COATED BIOCOMPATIBLE SURFACES Polyethylene glycol–coated biocompatible surfaces Norma A. Alcantar, Eray S. Aydil, Jacob N. Israelachvili Chemical Engineering Department and Materials Department, University of California, Santa Barbara,California 93106
YEAST CELL CYCLE FLOW CYTOMETRY PROTOCOL Protocol. Spin down 10 7 cells from an exponentially growing culture - 2000 rpm for 5 mins. Pour off supernatant. Vortex tube while adding 1.0 ml cold 70% EtOH. Store at 4 °C (cells keep ~indefinitely). When you want to process the cells, take 0.3 ml (this will be 2-3 x 10 6 cells, assuming a little loss in the washing) and add to 3 ml 50 mM ADHESION ASSAY PROTOCOL Adhesion Assay Protocol: Materials to be prepared beforehand: 1) Washing Buffer--0.1% BSA in medium (DMEM or RPMI) 2) Blocking Buffer--0.5% BSA in medium (DMEM or RPMI) EDUCATION GUIDE SPECIAL STAINS 1 Introduction to Special Stains Sonja Wulff Thousands of years ago, diagnosing and treating a patient’s condition was much simpler. Ancient Egyptians blamed disease on demons, Greeks and Romans pointed THMR-IP5680 HP I-LINE PHOTORESIST 4 26-WE4C03-00 OHKA AMERICA, INC. 300 400 500 600 700 800 900 300 400 500 600 700 800 900 Mask CD (nm) Print CD (nm) THMR-iP5680 HP Target CD = 0.6 µm, exposure dose = 160 ms EFFECT OF TEMPERATURE VARIATION ON THE VISIBLE AND NEAR 226 D. Cozzolino et al. / Analytica Chimica Acta 588 (2007) 224–230 Four wavelength regions were analysed in order to spectrally evaluate the effect of the temperature on the NIR wine spec-trum. These regions were, 950–1000nm, 1410–1470nm related 2D PROCESS MODELING WITH SILVACO ATHENA DR. LYNN FULLER Page 6 © April 30, 2014 Dr. Lynn Fuller, Professor Process Modeling - TCAD Page 11 SILVACO ATHENA SIMULATIONS OF D/S IMPLANT © April 30, 2014 Dr. Lynn Fuller, Professor BASIC LABORATORY PRACTICES Basic Laboratory Practices. General Method for Cleavage of His-Tagged Proteins with Thrombin cleavage sites . Thrombin recognises the consensus sequence Leu-Val-Pro-Arg-Gly-Ser, cleaving the peptide bond between Arg and Gly. This is utilised in many vector systems which encode such a protease cleavage site allowing removal of an upstreamdomain.
PLANT ELECTROPHYSIOLOGY Preface Plant electrophysiology is the study of the electrochemical phenomena associated with biological cells and tissues in plants. It involves measurements of electrical PHOTOINITIATORS FOR UVCURING 200 220 240 260 280 300 320 340 1.2 1.0 0.8 0.6 0.4 0.2 0.0 Key products, surface cure Absorption Spectra 0.001% (Acetonitrile) Wavelength (nm) Absorbance IRGACURE 184 DAROCUR 1173 IRGACURE 754 IRGACURE 2959 200 220 240 260 280 300 320 340 360 380 POLYETHYLENE GLYCOL-COATED BIOCOMPATIBLE SURFACES Polyethylene glycol–coated biocompatible surfaces Norma A. Alcantar, Eray S. Aydil, Jacob N. Israelachvili Chemical Engineering Department and Materials Department, University of California, Santa Barbara,California 93106
YEAST CELL CYCLE FLOW CYTOMETRY PROTOCOL Protocol. Spin down 10 7 cells from an exponentially growing culture - 2000 rpm for 5 mins. Pour off supernatant. Vortex tube while adding 1.0 ml cold 70% EtOH. Store at 4 °C (cells keep ~indefinitely). When you want to process the cells, take 0.3 ml (this will be 2-3 x 10 6 cells, assuming a little loss in the washing) and add to 3 ml 50 mM ADHESION ASSAY PROTOCOL Adhesion Assay Protocol: Materials to be prepared beforehand: 1) Washing Buffer--0.1% BSA in medium (DMEM or RPMI) 2) Blocking Buffer--0.5% BSA in medium (DMEM or RPMI) EDUCATION GUIDE SPECIAL STAINS 1 Introduction to Special Stains Sonja Wulff Thousands of years ago, diagnosing and treating a patient’s condition was much simpler. Ancient Egyptians blamed disease on demons, Greeks and Romans pointed THMR-IP5680 HP I-LINE PHOTORESIST 4 26-WE4C03-00 OHKA AMERICA, INC. 300 400 500 600 700 800 900 300 400 500 600 700 800 900 Mask CD (nm) Print CD (nm) THMR-iP5680 HP Target CD = 0.6 µm, exposure dose = 160 ms EFFECT OF TEMPERATURE VARIATION ON THE VISIBLE AND NEAR 226 D. Cozzolino et al. / Analytica Chimica Acta 588 (2007) 224–230 Four wavelength regions were analysed in order to spectrally evaluate the effect of the temperature on the NIR wine spec-trum. These regions were, 950–1000nm, 1410–1470nm related 2D PROCESS MODELING WITH SILVACO ATHENA DR. LYNN FULLER Page 6 © April 30, 2014 Dr. Lynn Fuller, Professor Process Modeling - TCAD Page 11 SILVACO ATHENA SIMULATIONS OF D/S IMPLANT © April 30, 2014 Dr. Lynn Fuller, ProfessorPCR TROUBLESHOOTING
Increase primer amount. Increase template amount. Decrease annealing temperature by 6-10º C and check if you get any product. If you don't, check all your PCR ingredients. If you do get products (including unspecific ones) reaction conditions as described above. Combine some/all of the above. 4. YEAST CELL CYCLE FLOW CYTOMETRY PROTOCOL Protocol. Spin down 10 7 cells from an exponentially growing culture - 2000 rpm for 5 mins. Pour off supernatant. Vortex tube while adding 1.0 ml cold 70% EtOH. Store at 4 °C (cells keep ~indefinitely). When you want to process the cells, take 0.3 ml (this will be 2-3 x 10 6 cells, assuming a little loss in the washing) and add to 3 ml 50 mM PROTEIN DETERMINATION BY THE BIURET METHOD Prepare a reference tube with 1 ml buffer. If possible, dilute unknowns to an estimated 1 to 10 mg/ml with buffer; a range of dilutions should be used if the actual concentration cannot be estimated. Use 1 ml sample per assay tube. Add 9 ml Biuret reagent to each tube, vortex immediately, and EDUCATION GUIDE SPECIAL STAINS 1 Introduction to Special Stains Sonja Wulff Thousands of years ago, diagnosing and treating a patient’s condition was much simpler. Ancient Egyptians blamed disease on demons, Greeks and Romans pointed THMR-IP5680 HP I-LINE PHOTORESIST 4 26-WE4C03-00 OHKA AMERICA, INC. 300 400 500 600 700 800 900 300 400 500 600 700 800 900 Mask CD (nm) Print CD (nm) THMR-iP5680 HP Target CD = 0.6 µm, exposure dose = 160 ms SPIN COATING: ONEDIMENSIONAL MODEL Spin coating: Onedimensional model D. E. Bornside, C. W. Macosko, and L. E. Scriven Citation: Journal of Applied Physics 66, 5185 (1989); doi: 10.1063/1.343754OPEN-SPECTROMETER
design files for a low-cost open source photospectrometer: root: summary refs log tree commit diffPINY-COMMANDS
CGI forms for Piny: root: summary refs log tree commit diff INTRODUCTION TO ION IMPLANTATION DR. LYNN FULLER, DR January 20, 2012 Dr. Lynn Fuller Rochester Institute of Technology Microelectronic Engineering Ion Implantation Page 2 VARIAN 400 & 120-10 ION IMPLANTERS FREQUENCY RESPONSE OF THE CE AMPLIFIER September 25, 2014 Dr. Lynn Fuller, Professor Rochester Institute of Technology Microelectronic Engineering Frequency Response Page 3INTRODUCTION
BASIC LABORATORY PRACTICES Basic Laboratory Practices. General Method for Cleavage of His-Tagged Proteins with Thrombin cleavage sites . Thrombin recognises the consensus sequence Leu-Val-Pro-Arg-Gly-Ser, cleaving the peptide bond between Arg and Gly. This is utilised in many vector systems which encode such a protease cleavage site allowing removal of an upstreamdomain.
PLANT ELECTROPHYSIOLOGY Preface Plant electrophysiology is the study of the electrochemical phenomena associated with biological cells and tissues in plants. It involves measurements of electrical PHOTOINITIATORS FOR UVCURING 200 220 240 260 280 300 320 340 1.2 1.0 0.8 0.6 0.4 0.2 0.0 Key products, surface cure Absorption Spectra 0.001% (Acetonitrile) Wavelength (nm) Absorbance IRGACURE 184 DAROCUR 1173 IRGACURE 754 IRGACURE 2959 200 220 240 260 280 300 320 340 360 380 POLYETHYLENE GLYCOL-COATED BIOCOMPATIBLE SURFACES Polyethylene glycol–coated biocompatible surfaces Norma A. Alcantar, Eray S. Aydil, Jacob N. Israelachvili Chemical Engineering Department and Materials Department, University of California, Santa Barbara,California 93106
YEAST CELL CYCLE FLOW CYTOMETRY PROTOCOL Protocol. Spin down 10 7 cells from an exponentially growing culture - 2000 rpm for 5 mins. Pour off supernatant. Vortex tube while adding 1.0 ml cold 70% EtOH. Store at 4 °C (cells keep ~indefinitely). When you want to process the cells, take 0.3 ml (this will be 2-3 x 10 6 cells, assuming a little loss in the washing) and add to 3 ml 50 mM ADHESION ASSAY PROTOCOL Adhesion Assay Protocol: Materials to be prepared beforehand: 1) Washing Buffer--0.1% BSA in medium (DMEM or RPMI) 2) Blocking Buffer--0.5% BSA in medium (DMEM or RPMI) EDUCATION GUIDE SPECIAL STAINS 1 Introduction to Special Stains Sonja Wulff Thousands of years ago, diagnosing and treating a patient’s condition was much simpler. Ancient Egyptians blamed disease on demons, Greeks and Romans pointed THMR-IP5680 HP I-LINE PHOTORESIST 4 26-WE4C03-00 OHKA AMERICA, INC. 300 400 500 600 700 800 900 300 400 500 600 700 800 900 Mask CD (nm) Print CD (nm) THMR-iP5680 HP Target CD = 0.6 µm, exposure dose = 160 ms EFFECT OF TEMPERATURE VARIATION ON THE VISIBLE AND NEAR 226 D. Cozzolino et al. / Analytica Chimica Acta 588 (2007) 224–230 Four wavelength regions were analysed in order to spectrally evaluate the effect of the temperature on the NIR wine spec-trum. These regions were, 950–1000nm, 1410–1470nm related 2D PROCESS MODELING WITH SILVACO ATHENA DR. LYNN FULLER Page 6 © April 30, 2014 Dr. Lynn Fuller, Professor Process Modeling - TCAD Page 11 SILVACO ATHENA SIMULATIONS OF D/S IMPLANT © April 30, 2014 Dr. Lynn Fuller, Professor BASIC LABORATORY PRACTICES Basic Laboratory Practices. General Method for Cleavage of His-Tagged Proteins with Thrombin cleavage sites . Thrombin recognises the consensus sequence Leu-Val-Pro-Arg-Gly-Ser, cleaving the peptide bond between Arg and Gly. This is utilised in many vector systems which encode such a protease cleavage site allowing removal of an upstreamdomain.
PLANT ELECTROPHYSIOLOGY Preface Plant electrophysiology is the study of the electrochemical phenomena associated with biological cells and tissues in plants. It involves measurements of electrical PHOTOINITIATORS FOR UVCURING 200 220 240 260 280 300 320 340 1.2 1.0 0.8 0.6 0.4 0.2 0.0 Key products, surface cure Absorption Spectra 0.001% (Acetonitrile) Wavelength (nm) Absorbance IRGACURE 184 DAROCUR 1173 IRGACURE 754 IRGACURE 2959 200 220 240 260 280 300 320 340 360 380 POLYETHYLENE GLYCOL-COATED BIOCOMPATIBLE SURFACES Polyethylene glycol–coated biocompatible surfaces Norma A. Alcantar, Eray S. Aydil, Jacob N. Israelachvili Chemical Engineering Department and Materials Department, University of California, Santa Barbara,California 93106
YEAST CELL CYCLE FLOW CYTOMETRY PROTOCOL Protocol. Spin down 10 7 cells from an exponentially growing culture - 2000 rpm for 5 mins. Pour off supernatant. Vortex tube while adding 1.0 ml cold 70% EtOH. Store at 4 °C (cells keep ~indefinitely). When you want to process the cells, take 0.3 ml (this will be 2-3 x 10 6 cells, assuming a little loss in the washing) and add to 3 ml 50 mM ADHESION ASSAY PROTOCOL Adhesion Assay Protocol: Materials to be prepared beforehand: 1) Washing Buffer--0.1% BSA in medium (DMEM or RPMI) 2) Blocking Buffer--0.5% BSA in medium (DMEM or RPMI) EDUCATION GUIDE SPECIAL STAINS 1 Introduction to Special Stains Sonja Wulff Thousands of years ago, diagnosing and treating a patient’s condition was much simpler. Ancient Egyptians blamed disease on demons, Greeks and Romans pointed THMR-IP5680 HP I-LINE PHOTORESIST 4 26-WE4C03-00 OHKA AMERICA, INC. 300 400 500 600 700 800 900 300 400 500 600 700 800 900 Mask CD (nm) Print CD (nm) THMR-iP5680 HP Target CD = 0.6 µm, exposure dose = 160 ms EFFECT OF TEMPERATURE VARIATION ON THE VISIBLE AND NEAR 226 D. Cozzolino et al. / Analytica Chimica Acta 588 (2007) 224–230 Four wavelength regions were analysed in order to spectrally evaluate the effect of the temperature on the NIR wine spec-trum. These regions were, 950–1000nm, 1410–1470nm related 2D PROCESS MODELING WITH SILVACO ATHENA DR. LYNN FULLER Page 6 © April 30, 2014 Dr. Lynn Fuller, Professor Process Modeling - TCAD Page 11 SILVACO ATHENA SIMULATIONS OF D/S IMPLANT © April 30, 2014 Dr. Lynn Fuller, ProfessorPCR TROUBLESHOOTING
Increase primer amount. Increase template amount. Decrease annealing temperature by 6-10º C and check if you get any product. If you don't, check all your PCR ingredients. If you do get products (including unspecific ones) reaction conditions as described above. Combine some/all of the above. 4. YEAST CELL CYCLE FLOW CYTOMETRY PROTOCOL Protocol. Spin down 10 7 cells from an exponentially growing culture - 2000 rpm for 5 mins. Pour off supernatant. Vortex tube while adding 1.0 ml cold 70% EtOH. Store at 4 °C (cells keep ~indefinitely). When you want to process the cells, take 0.3 ml (this will be 2-3 x 10 6 cells, assuming a little loss in the washing) and add to 3 ml 50 mM PROTEIN DETERMINATION BY THE BIURET METHOD Prepare a reference tube with 1 ml buffer. If possible, dilute unknowns to an estimated 1 to 10 mg/ml with buffer; a range of dilutions should be used if the actual concentration cannot be estimated. Use 1 ml sample per assay tube. Add 9 ml Biuret reagent to each tube, vortex immediately, and EDUCATION GUIDE SPECIAL STAINS 1 Introduction to Special Stains Sonja Wulff Thousands of years ago, diagnosing and treating a patient’s condition was much simpler. Ancient Egyptians blamed disease on demons, Greeks and Romans pointed THMR-IP5680 HP I-LINE PHOTORESIST 4 26-WE4C03-00 OHKA AMERICA, INC. 300 400 500 600 700 800 900 300 400 500 600 700 800 900 Mask CD (nm) Print CD (nm) THMR-iP5680 HP Target CD = 0.6 µm, exposure dose = 160 msOPEN-SPECTROMETER
design files for a low-cost open source photospectrometer: root: summary refs log tree commit diff SPIN COATING: ONEDIMENSIONAL MODEL Spin coating: Onedimensional model D. E. Bornside, C. W. Macosko, and L. E. Scriven Citation: Journal of Applied Physics 66, 5185 (1989); doi: 10.1063/1.343754PINY-COMMANDS
CGI forms for Piny: root: summary refs log tree commit diff INTRODUCTION TO ION IMPLANTATION DR. LYNN FULLER, DR January 20, 2012 Dr. Lynn Fuller Rochester Institute of Technology Microelectronic Engineering Ion Implantation Page 2 VARIAN 400 & 120-10 ION IMPLANTERS FREQUENCY RESPONSE OF THE CE AMPLIFIER September 25, 2014 Dr. Lynn Fuller, Professor Rochester Institute of Technology Microelectronic Engineering Frequency Response Page 3INTRODUCTION
BASIC LABORATORY PRACTICES Basic Laboratory Practices. General Method for Cleavage of His-Tagged Proteins with Thrombin cleavage sites . Thrombin recognises the consensus sequence Leu-Val-Pro-Arg-Gly-Ser, cleaving the peptide bond between Arg and Gly. This is utilised in many vector systems which encode such a protease cleavage site allowing removal of an upstreamdomain.
PCR TROUBLESHOOTING
Increase primer amount. Increase template amount. Decrease annealing temperature by 6-10º C and check if you get any product. If you don't, check all your PCR ingredients. If you do get products (including unspecific ones) reaction conditions as described above. Combine some/all of the above. 4. THMR-IP5680 HP I-LINE PHOTORESIST 4 26-WE4C03-00 OHKA AMERICA, INC. 300 400 500 600 700 800 900 300 400 500 600 700 800 900 Mask CD (nm) Print CD (nm) THMR-iP5680 HP Target CD = 0.6 µm, exposure dose = 160 ms YEAST CELL CYCLE FLOW CYTOMETRY PROTOCOL Protocol. Spin down 10 7 cells from an exponentially growing culture - 2000 rpm for 5 mins. Pour off supernatant. Vortex tube while adding 1.0 ml cold 70% EtOH. Store at 4 °C (cells keep ~indefinitely). When you want to process the cells, take 0.3 ml (this will be 2-3 x 10 6 cells, assuming a little loss in the washing) and add to 3 ml 50 mM EDUCATION GUIDE SPECIAL STAINS 1 Introduction to Special Stains Sonja Wulff Thousands of years ago, diagnosing and treating a patient’s condition was much simpler. Ancient Egyptians blamed disease on demons, Greeks and Romans pointed A LOW-COST, OPEN-SOURCE DIY DNA SYNTHESIZER PROJECT Building low-cost DNA synthesizers Our project has multiple phases, but the most interesting phase is the microfluidic chip. Microscopic channels guide the direction of fluids and reagents. Version 1: phosphoramidite chemistry. Version “the next one”: ligation chemistry from on- chip 4,096 droplet library (synthesis by shortoligo
GROWTH AND CHARACTERIZATION OF (110) INAS QUANTUM WELL Growth and Characterization of (110) InAs Quantum Well Heterostructures by Transmission Electron Microscopy and Electron Channeling Contrast Imaging. CLINICAL DEVELOPMENT SUCCESS RATES FOR INVESTIGATIONAL DRUGS 42 volume 32 NumBeR 1 JANuARY 2014 nature biotechnology data to annotate drugs by their biochemi-cal composition (e.g., peptide, nucleic acid, monoclonal antibody (mAb)) and molecu-lar size (i.e., large and small molecules). ADVANTAGES OF BEAM DECELERATION FOR LOW KV EDS ANALYSIS. Figure 1. EDS map of Slag at 3kV and 90nA: (a,c,e) No Gentle Beam; (b,d,f) -5kV Gentle Beam. a b e f c d a Microsc. Microanal. 21 (Suppl3), 2015 682
HIGH SPEED LOGIC CIRCUITS DR. LYNN FULLER November 5, 2014 Dr. Lynn Fuller High Speed Logic Page 3 Rochester Institute of Technology Microelectronic Engineering OUTLINEIntroduction
BASIC LABORATORY PRACTICES Basic Laboratory Practices. General Method for Cleavage of His-Tagged Proteins with Thrombin cleavage sites . Thrombin recognises the consensus sequence Leu-Val-Pro-Arg-Gly-Ser, cleaving the peptide bond between Arg and Gly. This is utilised in many vector systems which encode such a protease cleavage site allowing removal of an upstreamdomain.
PCR TROUBLESHOOTING
Increase primer amount. Increase template amount. Decrease annealing temperature by 6-10º C and check if you get any product. If you don't, check all your PCR ingredients. If you do get products (including unspecific ones) reaction conditions as described above. Combine some/all of the above. 4. YEAST CELL CYCLE FLOW CYTOMETRY PROTOCOL Protocol. Spin down 10 7 cells from an exponentially growing culture - 2000 rpm for 5 mins. Pour off supernatant. Vortex tube while adding 1.0 ml cold 70% EtOH. Store at 4 °C (cells keep ~indefinitely). When you want to process the cells, take 0.3 ml (this will be 2-3 x 10 6 cells, assuming a little loss in the washing) and add to 3 ml 50 mM THMR-IP5680 HP I-LINE PHOTORESIST 4 26-WE4C03-00 OHKA AMERICA, INC. 300 400 500 600 700 800 900 300 400 500 600 700 800 900 Mask CD (nm) Print CD (nm) THMR-iP5680 HP Target CD = 0.6 µm, exposure dose = 160 ms EDUCATION GUIDE SPECIAL STAINS 1 Introduction to Special Stains Sonja Wulff Thousands of years ago, diagnosing and treating a patient’s condition was much simpler. Ancient Egyptians blamed disease on demons, Greeks and Romans pointed A LOW-COST, OPEN-SOURCE DIY DNA SYNTHESIZER PROJECT Building low-cost DNA synthesizers Our project has multiple phases, but the most interesting phase is the microfluidic chip. Microscopic channels guide the direction of fluids and reagents. Version 1: phosphoramidite chemistry. Version “the next one”: ligation chemistry from on- chip 4,096 droplet library (synthesis by shortoligo
GROWTH AND CHARACTERIZATION OF (110) INAS QUANTUM WELL Growth and Characterization of (110) InAs Quantum Well Heterostructures by Transmission Electron Microscopy and Electron Channeling Contrast Imaging. CLINICAL DEVELOPMENT SUCCESS RATES FOR INVESTIGATIONAL DRUGS 42 volume 32 NumBeR 1 JANuARY 2014 nature biotechnology data to annotate drugs by their biochemi-cal composition (e.g., peptide, nucleic acid, monoclonal antibody (mAb)) and molecu-lar size (i.e., large and small molecules). ADVANTAGES OF BEAM DECELERATION FOR LOW KV EDS ANALYSIS. Figure 1. EDS map of Slag at 3kV and 90nA: (a,c,e) No Gentle Beam; (b,d,f) -5kV Gentle Beam. a b e f c d a Microsc. Microanal. 21 (Suppl3), 2015 682
HIGH SPEED LOGIC CIRCUITS DR. LYNN FULLER November 5, 2014 Dr. Lynn Fuller High Speed Logic Page 3 Rochester Institute of Technology Microelectronic Engineering OUTLINEIntroduction
PCR TROUBLESHOOTING
Increase primer amount. Increase template amount. Decrease annealing temperature by 6-10º C and check if you get any product. If you don't, check all your PCR ingredients. If you do get products (including unspecific ones) reaction conditions as described above. Combine some/all of the above. 4. YEAST CELL CYCLE FLOW CYTOMETRY PROTOCOL Protocol. Spin down 10 7 cells from an exponentially growing culture - 2000 rpm for 5 mins. Pour off supernatant. Vortex tube while adding 1.0 ml cold 70% EtOH. Store at 4 °C (cells keep ~indefinitely). When you want to process the cells, take 0.3 ml (this will be 2-3 x 10 6 cells, assuming a little loss in the washing) and add to 3 ml 50 mMEISENLAB - DIYHPL
Maple Tree is a java-based, open source, cross-platform visualization tool to graphically browse the results of clustering analyses from our Cluster and Fuzzy K clustering software, and many other clustering and analysis programs. Maple Tree was developed by Lisa Simirenko in our lab. Combined Expression Data and Sequence Analysis. PHOTOINITIATORS FOR UVCURING 200 220 240 260 280 300 320 340 1.2 1.0 0.8 0.6 0.4 0.2 0.0 Key products, surface cure Absorption Spectra 0.001% (Acetonitrile) Wavelength (nm) Absorbance IRGACURE 184 DAROCUR 1173 IRGACURE 754 IRGACURE 2959 200 220 240 260 280 300 320 340 360 380 EDUCATION GUIDE SPECIAL STAINS 1 Introduction to Special Stains Sonja Wulff Thousands of years ago, diagnosing and treating a patient’s condition was much simpler. Ancient Egyptians blamed disease on demons, Greeks and Romans pointed POLYETHYLENE GLYCOL-COATED BIOCOMPATIBLE SURFACES Polyethylene glycol–coated biocompatible surfaces Norma A. Alcantar, Eray S. Aydil, Jacob N. Israelachvili Chemical Engineering Department and Materials Department, University of California, Santa Barbara,California 93106
OPEN-SPECTROMETER
design files for a low-cost open source photospectrometer: root: summary refs log tree commit diffPINY-COMMANDS
CGI forms for Piny: root: summary refs log tree commit diff CLINICAL DEVELOPMENT SUCCESS RATES FOR INVESTIGATIONAL DRUGS 42 volume 32 NumBeR 1 JANuARY 2014 nature biotechnology data to annotate drugs by their biochemi-cal composition (e.g., peptide, nucleic acid, monoclonal antibody (mAb)) and molecu-lar size (i.e., large and small molecules). PORTLAB - BIOHACKING WIKI FOR PORTLAND, OREGON. Biohacking wiki for Portland, Oregon. root: summary refs log treecommit diff
BASIC LABORATORY PRACTICES General Method for Cleavage of His-Tagged Proteins with Thrombin cleavage sites.. Thrombin recognises the consensus sequence Leu-Val-Pro-Arg-Gly-Ser, EISENLAB - DIYHPL.US All software produced by our lab may be downloaded and used free of charge by academic and other non-profit researchers. Commercial use of the ScanAlyze, Cluster and/or TreeView executable and/or source code requries a license from Stanford University. EDUCATION GUIDE SPECIAL STAINS 1 Introduction to Special Stains Sonja Wulff Thousands of years ago, diagnosing and treating a patient’s condition was much simpler. Ancient Egyptians blamed disease on demons, Greeks and Romans pointed YEAST CELL CYCLE FLOW CYTOMETRY PROTOCOL Reagents. Cold absolute ethanol. 0.5 M Na citrate stock (filtered), 50mM diluted stock. 10 mg/ml RNase A (Boil 10 mins, cool, filter andstore at -20°C).
A LOW-COST, OPEN-SOURCE DIY DNA SYNTHESIZER PROJECT Building low-cost DNA synthesizers Our project has multiple phases, but the most interesting phase is the microfluidic chip. Microscopic channels guide the direction of fluids and reagents. Version 1: phosphoramidite chemistry. Version “the next one”: ligation chemistry from on- chip 4,096 droplet library (synthesis by shortoligo
GROWTH AND CHARACTERIZATION OF (110) INAS QUANTUM WELL Growth and Characterization of (110) InAs Quantum Well Heterostructures by Transmission Electron Microscopy and Electron Channeling Contrast Imaging. HIGH SPEED LOGIC CIRCUITS DR. LYNN FULLER November 5, 2014 Dr. Lynn Fuller High Speed Logic Page 3 Rochester Institute of Technology Microelectronic Engineering OUTLINEIntroduction
CLINICAL DEVELOPMENT SUCCESS RATES FOR INVESTIGATIONAL DRUGS 42 volume 32 NumBeR 1 JANuARY 2014 nature biotechnology data to annotate drugs by their biochemi-cal composition (e.g., peptide, nucleic acid, monoclonal antibody (mAb)) and molecu-lar size (i.e., large and small molecules). ADVANTAGES OF BEAM DECELERATION FOR LOW KV EDS ANALYSIS. Figure 1. EDS map of Slag at 3kV and 90nA: (a,c,e) No Gentle Beam; (b,d,f) -5kV Gentle Beam. a b e f c d a Microsc. Microanal. 21 (Suppl3), 2015 682
A PATHETICALLY SIMPLE CHARGE SENSITIVE AMPLIFIER A Pathetically Simple Charge Sensitive Amplifier The schematic above shows an extremely simple charge sensitive amplifier with only twoactive components.
BASIC LABORATORY PRACTICES General Method for Cleavage of His-Tagged Proteins with Thrombin cleavage sites.. Thrombin recognises the consensus sequence Leu-Val-Pro-Arg-Gly-Ser, EISENLAB - DIYHPL.US All software produced by our lab may be downloaded and used free of charge by academic and other non-profit researchers. Commercial use of the ScanAlyze, Cluster and/or TreeView executable and/or source code requries a license from Stanford University. EDUCATION GUIDE SPECIAL STAINS 1 Introduction to Special Stains Sonja Wulff Thousands of years ago, diagnosing and treating a patient’s condition was much simpler. Ancient Egyptians blamed disease on demons, Greeks and Romans pointed YEAST CELL CYCLE FLOW CYTOMETRY PROTOCOL Reagents. Cold absolute ethanol. 0.5 M Na citrate stock (filtered), 50mM diluted stock. 10 mg/ml RNase A (Boil 10 mins, cool, filter andstore at -20°C).
A LOW-COST, OPEN-SOURCE DIY DNA SYNTHESIZER PROJECT Building low-cost DNA synthesizers Our project has multiple phases, but the most interesting phase is the microfluidic chip. Microscopic channels guide the direction of fluids and reagents. Version 1: phosphoramidite chemistry. Version “the next one”: ligation chemistry from on- chip 4,096 droplet library (synthesis by shortoligo
GROWTH AND CHARACTERIZATION OF (110) INAS QUANTUM WELL Growth and Characterization of (110) InAs Quantum Well Heterostructures by Transmission Electron Microscopy and Electron Channeling Contrast Imaging. HIGH SPEED LOGIC CIRCUITS DR. LYNN FULLER November 5, 2014 Dr. Lynn Fuller High Speed Logic Page 3 Rochester Institute of Technology Microelectronic Engineering OUTLINEIntroduction
CLINICAL DEVELOPMENT SUCCESS RATES FOR INVESTIGATIONAL DRUGS 42 volume 32 NumBeR 1 JANuARY 2014 nature biotechnology data to annotate drugs by their biochemi-cal composition (e.g., peptide, nucleic acid, monoclonal antibody (mAb)) and molecu-lar size (i.e., large and small molecules). ADVANTAGES OF BEAM DECELERATION FOR LOW KV EDS ANALYSIS. Figure 1. EDS map of Slag at 3kV and 90nA: (a,c,e) No Gentle Beam; (b,d,f) -5kV Gentle Beam. a b e f c d a Microsc. Microanal. 21 (Suppl3), 2015 682
A PATHETICALLY SIMPLE CHARGE SENSITIVE AMPLIFIER A Pathetically Simple Charge Sensitive Amplifier The schematic above shows an extremely simple charge sensitive amplifier with only twoactive components.
LINUXCNC - LINUXCNC - ENHANCED MACHINE CONTROLLER (EMC) linuxcnc - enhanced machine controller (EMC) root: summary refs logtree commit diff
PHOTOINITIATORS FOR UVCURING 200 220 240 260 280 300 320 340 1.2 1.0 0.8 0.6 0.4 0.2 0.0 Key products, surface cure Absorption Spectra 0.001% (Acetonitrile) Wavelength (nm) Absorbance IRGACURE 184 DAROCUR 1173 IRGACURE 754 IRGACURE 2959 200 220 240 260 280 300 320 340 360 380PCR TROUBLESHOOTING
Increase annealing temperature Increase annealing time Increase extension time Increase extension temperature to 74-78º C Decrease KCl (buffer) concentration to 0.7 PORTLAB - BIOHACKING WIKI FOR PORTLAND, OREGON. Biohacking wiki for Portland, Oregon. root: summary refs log treecommit diff
PINY-COMMANDS
CGI forms for Piny: root: summary refs log tree commit diffOPEN-SPECTROMETER
design files for a low-cost open source photospectrometer: root: summary refs log tree commit diff EDUCATION GUIDE SPECIAL STAINS 1 Introduction to Special Stains Sonja Wulff Thousands of years ago, diagnosing and treating a patient’s condition was much simpler. Ancient Egyptians blamed disease on demons, Greeks and Romans pointed POLYETHYLENE GLYCOL-COATED BIOCOMPATIBLE SURFACES Polyethylene glycol–coated biocompatible surfaces Norma A. Alcantar, Eray S. Aydil, Jacob N. Israelachvili Chemical Engineering Department and Materials Department, University of California, Santa Barbara,California 93106
YEAST CELL CYCLE FLOW CYTOMETRY PROTOCOL Reagents. Cold absolute ethanol. 0.5 M Na citrate stock (filtered), 50mM diluted stock. 10 mg/ml RNase A (Boil 10 mins, cool, filter andstore at -20°C).
PROTEIN DETERMINATION BY THE BIURET METHOD The color is stable, but all readings should be taken within 10 min. of each other. As with most assays, the Biuret can be scaled down for smaller cuvette sizes, consuming less protein.diyhpluswiki
* Edit
* RecentChanges
* History
* Preferences
ABOUT THIS WIKI
This is a wiki for open source hardware, do-it-yourself biohacking and practical transhumanism. The engineering teams behind this wiki can be found in the hplusroadmap IRC channel (logs ). Projects are self-funded by the group.EDITING THIS WIKI
Submit a pull request on github: https://github.com/kanzure/diyhpluswiki Register to edit here and see other commands . You can also clone this wiki with git and push changes back: git clone git://diyhpl.us/diyhpluswiki.git Note to push changes you must push with your registered account: git push username@diyhpl.us:/srv/git/diyhpluswiki.git master You can make this easier by updating the remotes: git remote set-url origin username@diyhpl.us:/srv/git/diyhpluswiki.git Now "git push" should work without debilitating complaint. browse wiki history on cgitINTERESTING PAGES
* genetic-modifications - proposed humanupgrades
* gene-editing - various gene therapy tools andmechanisms
* protein-engineering - protein folding and protein engineering wishlist * transcripts - bryan goes to conferences and types really really fast * hplusroadmap - what is hplusroadmap? * projects - what can you do with DNA synthesis? * faq - this is a basic FAQ on do-it-yourselfbiohacking
* groups - stalk everyone in diybio for fun andprofit!
INDEX OF PAGES
* articles
* bitcoin
* cad
* chatsummaries
* diybio
* dna
* fda
* gene-editing
* gene-therapy
* genetic-modifications* goals.yaml.txt
* homecmos
* hplusroadmap
* human-modifications.csv* index
* longevity
* myostatin
* nanotech
* nootropics
* polymerase
* projects
* protein-engineering* proteins
* scaffolding
* self-replicating machines* senses
* talk:diybio
* trans-tech.yaml.txt* transcripts
* transhumanism
* users
* wikiicons
* wikis
OTHER
Also there's a laser_etcher for microfluidicsproject (see cgit
).
Last edited Thu May 13 13:10:38 2021Details
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