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fluorometry.
2019-NCOV CORONAVIRUS NGS REAGENTS Amplicon sequencing . Amplicon sequencing is a type of targeted next generation sequencing that uses PCR to create sequences of DNA called amplicons. It is simple, fast, and has a variety of applications. Tools to sequence the virus are available through ARTICnetwork, a network of researchers that are developing a system for processing virus samples to generate public health information . INTEGRATED DNA TECHNOLOGIES IDT product details for 5' TEX 615. 5' TEX 615 Return to Fluorophores Modifications. A red wavelength dye commonly used in multiplex reactions with FAM or in fluorescence-activated cell sorting (FACS) and microscopy applications using the rhodamine filter. INTEGRATED DNA TECHNOLOGIES ǀ IDTORDER HISTORYCUSTOM DNA OLIGOSPRODUCTS & SERVICESAPPLICATIONS & TECHNOLOGIES Prove it. We'll help. Our custom oligo synthesis platforms provide innovative research tools for genomics applications using NGS, CRISPR, qPCR, and synthetic biology NUCLEASE DETECTION AND CONTROL Reagents for rapid detection and elimination of RNases and DNases. The detection assays, which are effective for testing lab reagents, equipment, and other lab supplies, can be read visually for qualitative assessment of contamination or quantified usingfluorometry.
2019-NCOV CORONAVIRUS NGS REAGENTS Amplicon sequencing . Amplicon sequencing is a type of targeted next generation sequencing that uses PCR to create sequences of DNA called amplicons. It is simple, fast, and has a variety of applications. Tools to sequence the virus are available through ARTICnetwork, a network of researchers that are developing a system for processing virus samples to generate public health information . INTEGRATED DNA TECHNOLOGIES IDT product details for 5' TEX 615. 5' TEX 615 Return to Fluorophores Modifications. A red wavelength dye commonly used in multiplex reactions with FAM or in fluorescence-activated cell sorting (FACS) and microscopy applications using the rhodamine filter.OLIGOANALYZER TOOL
OligoAnalyzer is a primer analysis tool for oligonucleotides. Design and analyze DNA and RNA oligos for insight into behavior andproperties.
GEORGE CHURCH'S WOOLLY MAMMOTH PROJECT In ongoing research, Dr. Church is using IDT oligonucleotides, high-quality, double-stranded DNA, to explore the de-extinction of the woolly mammoth.“We use it for just about every project,” said Dr. Church about IDT’s synthetic DNA. By editing oligos manufactured to have specified characteristics of ancient woolly mammoth DNA with the Asian elephant genome, Dr. Church plans to use IDT CODON OPTIMIZATION TOOL The Codon Optimization Tool converts the DNA or protein sequence from one organism for expression in another. The IDT algorithm provides the best sequence option by screening and filtering sequences to lower complexity and minimize secondary structures. GBLOCKS GENE FRAGMENTS Pure confidence. gBlocks™ Gene Fragments are double-stranded DNA fragments of 125–3000 bp in length. They are the industry standard for double-stranded gene fragments, designed for affordable and easy gene construction or modification, applications such as antibody research and CRISPR-mediated genome editing, qPCR standards, and more. INTEGRATED DNA TECHNOLOGIES IDT product details for 5' Dual Biotin. 5' Dual Biotin Return to Attachment Chemistry / Linkers Modifications. Two biotin groups are sequentially placed on the 5’-end, which increases the efficiency of streptavidin binding. ALT-R CRISPR ENZYMES Alt-R S.p Cas9 nuclease; Alt-R S.p. Cas9 Nuclease V3 is the standard Cas9 used for general genome editing. It is a high purity, recombinant S. pyogenes Cas9. The enzymes include nuclear localization sequences (NLSs) and C-terminal 6-His tags. RHAMPSEQ DESIGN TOOL Tailored to your research. Custom rhAmpSeq Panels have been designed and tested in a wide range of animal and plant species and diverse targeted NGS applications, including:PRIMERQUEST
Web resources Design efficient PCR and qPCR primers and probes using online tools. The basics of designing customized primers and probes atIDT. Be sure to
TIPS FOR RESUSPENDING AND DILUTING OLIGONUCLEOTIDES Oligo resuspension. If you receive your oligos dry, you will need to resuspend them in aqueous solution before use. Most oligos are relatively easy to resuspend; however, those modified with fluorophores or hydrophobic molecules may require more time to become completely solubilized. LOTUS DNA LIBRARY PREP KIT FOR NGS Kit overview. The Lotus kit comes with all the buffers, reagents, and enzymes needed for fragmentation, adapter ligation, and an optional PCR (to add index sequences, depending on adapter type, or to increase the amount of DNA for sequencing). INTEGRATED DNA TECHNOLOGIES ǀ IDTORDER HISTORYCUSTOM DNA OLIGOSPRODUCTS & SERVICESAPPLICATIONS & TECHNOLOGIES Prove it. We'll help. Our custom oligo synthesis platforms provide innovative research tools for genomics applications using NGS, CRISPR, qPCR, and synthetic biologyPLEASE SIGN IN
Please sign in to use IDT’s custom online ordering tools. If you don’t yet have an IDT account, join the IDT community! Create your free account today and enjoy unlimited access to our innovative web tools, streamlined ordering, and expert educational content. PRIMETIME GENE EXPRESSION MASTER MIX Achieve quality performance under various experimental conditions with this thermostable probe-based qPCR master mix. PrimeTime Gene Expression Master Mix is a 2X solution containing a hot-start antibody, Taq polymerase, and other components needed INTEGRATED DNA TECHNOLOGIES Iowa Black® RQ. Iowa Black® RQ has a broad absorbance spectra ranging from 500 to 700 nm with peak absorbance at 656 nm. This quencher is ideal for use with Texas Red ®, Cy5 TM, and other fluorescent dyes that emit in the red spectral range.. View product details for Iowa Black® RQ:ULTRAMER DNA OLIGOS
Ultramer DNA Oligos are long, single- and double-stranded synthetic DNA sequences. They are manufactured with an optimized synthesis platform that achieves consistent coupling efficiencies over 99.5%. With their increased length and purity, Ultramer DNA oligos have increased utility in a variety of molecular biology applications, including (but not limited to): INTEGRATED DNA TECHNOLOGIES ǀ IDTORDER HISTORYCUSTOM DNA OLIGOSPRODUCTS & SERVICESAPPLICATIONS & TECHNOLOGIES Prove it. We'll help. Our custom oligo synthesis platforms provide innovative research tools for genomics applications using NGS, CRISPR, qPCR, and synthetic biologyPLEASE SIGN IN
Please sign in to use IDT’s custom online ordering tools. If you don’t yet have an IDT account, join the IDT community! Create your free account today and enjoy unlimited access to our innovative web tools, streamlined ordering, and expert educational content. PRIMETIME GENE EXPRESSION MASTER MIX Achieve quality performance under various experimental conditions with this thermostable probe-based qPCR master mix. PrimeTime Gene Expression Master Mix is a 2X solution containing a hot-start antibody, Taq polymerase, and other components needed INTEGRATED DNA TECHNOLOGIES Iowa Black® RQ. Iowa Black® RQ has a broad absorbance spectra ranging from 500 to 700 nm with peak absorbance at 656 nm. This quencher is ideal for use with Texas Red ®, Cy5 TM, and other fluorescent dyes that emit in the red spectral range.. View product details for Iowa Black® RQ:ULTRAMER DNA OLIGOS
Ultramer DNA Oligos are long, single- and double-stranded synthetic DNA sequences. They are manufactured with an optimized synthesis platform that achieves consistent coupling efficiencies over 99.5%. With their increased length and purity, Ultramer DNA oligos have increased utility in a variety of molecular biology applications, including (but not limited to):MGB ECLIPSE PROBES
MGB Eclipse ® probes and companion primers are manufactured under GMP conditions, which allows you to:. Transition from research to clinical diagnostics while maintaining robust assay performance; Begin your testing sooner with faster, more reliable delivery INTEGRATED DNA TECHNOLOGIES 3' C3 Spacer Return to Spacers Modifications. The C3 Spacer phosphoramidite can be incorporated internally or at the 5'-end of the oligo. Multiple C3 spacers can be added at either end of an oligo to introduce a long hydrophilic spacer arm for the attachment of fluorophores or other pendent groups. CUSTOM GENE SYNTHESIS FROM THE COMPANY YOU TRUST Sequence Information is secure and confidential at IDT. Please see our Confidentiality Statement for more information.. All Genes and MiniGene™ Synthetic Genes are constructed with either gBlocks ® Gene Fragments or Ultramer ® DNA Oligos, and are sequence verified by either Sanger or MiSeq™ system. The exact method used will be determined by IDT as part of the manufacturing process. NUCLEASE DETECTION AND CONTROL Reagents for rapid detection and elimination of RNases and DNases. The detection assays, which are effective for testing lab reagents, equipment, and other lab supplies, can be read visually for qualitative assessment of contamination or quantified usingfluorometry.
PRIMERQUEST
Web resources Design efficient PCR and qPCR primers and probes using online tools. The basics of designing customized primers and probes atIDT. Be sure to
ACCOUNT SETUP
You will be logged off in seconds due to inactivity. Click here to continue using this web page. ACCELERATE YOUR AMPLICON SEQUENCING Faster discovery with a rapid and accurate targeted sequencing solution. The rhAmpSeq system enables highly accurate amplicon sequencing on Illumina ® next generation sequencing (NGS) platforms. Whether you are investigating thousands of targets or a few, the fast and easy rhAmpSeq workflow, based on our proprietary RNase H2-dependent PCR (rhAmp PCR) technology, generates NGS-readyHOW TO ORDER
IDT offers many ordering and account features on the website including: Multiple oligo entry Convenient plate ordering Wish listfor saved items
LIBRARY PREPARATION
Library preparation is the first step of next generation sequencing. It allows DNA or RNA to adhere to the sequencing flowcell and allows the sample to be identified.MGB ECLIPSE PROBES
In the United States, MGB probes are not limited to human in vitro diagnostic use only. Although a number of other available applications exist, including in vitro diagnostics, commercial, agricultural biotechnology (agbio), and research, each customer who purchases this product is required to obtain their own regulatory approval for any use outside of research. HOW TO STORE PRIMERS: 7 THINGS TO KNOW Oligo stability in three storage mediums at –20°C, 4°C, and 37°C. The stability of oligos stored in TE buffer (IDTE, pH 8.0), nuclease-free water, or dried was assessed in a qPCR assay when each was stored at –20°C, 4 °C, and 37°C for varying lengths of time. The graphs plot the ΔC q for various time points, which is a sensitive indicator of loss of oligo function. IDEAS FOR SCIENCE-RELATED SHOWS, PODCASTS, VIDEOS, BOOKS We offer our science-themed suggestions for things to listen to, read, and watch. Learn about kids shows, science shows, podcasts, andjournal ratings.
INTEGRATED DNA TECHNOLOGIES ǀ IDTORDER HISTORYCUSTOM DNA OLIGOSPRODUCTS & SERVICESAPPLICATIONS & TECHNOLOGIES IDT acquires Swift Biosciences. Together we will deliver integrated NGS workflows for genomics, translational, and clinical research. Discovering a new biomarker is the first step in precision care for cancer. But, to find them, researchers need to sequence low-input, poor-quality samples. See how easy it NUCLEASE DETECTION AND CONTROL Nuclease detection and control. Reagents for rapid detection and elimination of RNases and DNases. The detection assays, which are effective for testing lab reagents, equipment, and other lab supplies, can be read visually for qualitative assessment of contamination or quantified using fluorometry. 2019-NCOV CORONAVIRUS NGS REAGENTS Targeted NGS is a valuable tool for both detection and characterization of viruses. Using very sensitive and accurate assays, we can monitor virus evolution, infection progression, and immune response. Hybridization capture and amplicon sequencing are both NGS PRIMETIME GENE EXPRESSION MASTER MIX Achieve quality performance under various experimental conditions with this thermostable probe-based qPCR master mix. PrimeTime Gene Expression Master Mix is a 2X solution containing a hot-start antibody, Taq polymerase, and other components needed EXOME RESEARCH PANEL The xGen Exome Research Panel v2 gives you the confidence that only individual probe synthesis and individual QC can provide, and it comes at a price that makes your choice easy. The new standard for exome coverage. Consistent performance through time. INTEGRATED DNA TECHNOLOGIES 5 umole RNA Oligo. $990.00 USD. Add To Order. 10 umole RNA Oligo. $2,000.00 USD. Add To Order. 100 nmole DNA Plate Oligo. $250.00 USD.Add To Order.
INTEGRATED DNA TECHNOLOGIES 5' TEX 615. Return to Fluorophores Modifications. A red wavelength dye commonly used in multiplex reactions with FAM or in fluorescence-activated cell sorting (FACS) and microscopy applications using the rhodamine filter. TEX 615 can be used as a direct substitute for Texas Red or CAL Fluor 610. Scientific Details. HOW CAN I CHECK MY ORDER STATUS? How can I check my order status? Checking the status of your order is as easy as logging into your account at our website and checking your order history via www.idtdna.com > Order > Order History. Alternatively, you can can contact IDT customer care by phone, email,or web chat.
CRISPR TECHNOLOGY IS BEING USED FOR VACCINE DEVELOPMENT Leishmaniasis is the type of disease you really don’t want to get. Caused by a parasite spread by sandflies, cases first appear as bumps or lumps which may end up as ulcers, which no less than the U.S. Centers for Disease Control and Prevention described as “a volcano.” One form of the disease can wreak havoc on internal organs like the liver, spleen, and bone marrow—and can do so HOW DO 5' PHOSPHORYLATED PRIMERS AFFECT PCR? Primers that are 5' phosphorylated do not affect PCR since primer extension occurs from the 3' end of the primer. 5' phosphates on each strand of the amplicon are INTEGRATED DNA TECHNOLOGIES ǀ IDTORDER HISTORYCUSTOM DNA OLIGOSPRODUCTS & SERVICESAPPLICATIONS & TECHNOLOGIES IDT acquires Swift Biosciences. Together we will deliver integrated NGS workflows for genomics, translational, and clinical research. Discovering a new biomarker is the first step in precision care for cancer. But, to find them, researchers need to sequence low-input, poor-quality samples. See how easy it NUCLEASE DETECTION AND CONTROL Nuclease detection and control. Reagents for rapid detection and elimination of RNases and DNases. The detection assays, which are effective for testing lab reagents, equipment, and other lab supplies, can be read visually for qualitative assessment of contamination or quantified using fluorometry. 2019-NCOV CORONAVIRUS NGS REAGENTS Targeted NGS is a valuable tool for both detection and characterization of viruses. Using very sensitive and accurate assays, we can monitor virus evolution, infection progression, and immune response. Hybridization capture and amplicon sequencing are both NGS PRIMETIME GENE EXPRESSION MASTER MIX Achieve quality performance under various experimental conditions with this thermostable probe-based qPCR master mix. PrimeTime Gene Expression Master Mix is a 2X solution containing a hot-start antibody, Taq polymerase, and other components needed EXOME RESEARCH PANEL The xGen Exome Research Panel v2 gives you the confidence that only individual probe synthesis and individual QC can provide, and it comes at a price that makes your choice easy. The new standard for exome coverage. Consistent performance through time. INTEGRATED DNA TECHNOLOGIES 5 umole RNA Oligo. $990.00 USD. Add To Order. 10 umole RNA Oligo. $2,000.00 USD. Add To Order. 100 nmole DNA Plate Oligo. $250.00 USD.Add To Order.
INTEGRATED DNA TECHNOLOGIES 5' TEX 615. Return to Fluorophores Modifications. A red wavelength dye commonly used in multiplex reactions with FAM or in fluorescence-activated cell sorting (FACS) and microscopy applications using the rhodamine filter. TEX 615 can be used as a direct substitute for Texas Red or CAL Fluor 610. Scientific Details. HOW CAN I CHECK MY ORDER STATUS? How can I check my order status? Checking the status of your order is as easy as logging into your account at our website and checking your order history via www.idtdna.com > Order > Order History. Alternatively, you can can contact IDT customer care by phone, email,or web chat.
CRISPR TECHNOLOGY IS BEING USED FOR VACCINE DEVELOPMENT Leishmaniasis is the type of disease you really don’t want to get. Caused by a parasite spread by sandflies, cases first appear as bumps or lumps which may end up as ulcers, which no less than the U.S. Centers for Disease Control and Prevention described as “a volcano.” One form of the disease can wreak havoc on internal organs like the liver, spleen, and bone marrow—and can do so HOW DO 5' PHOSPHORYLATED PRIMERS AFFECT PCR? Primers that are 5' phosphorylated do not affect PCR since primer extension occurs from the 3' end of the primer. 5' phosphates on each strand of the amplicon are NUCLEASE DETECTION AND CONTROL Nuclease detection and control. Reagents for rapid detection and elimination of RNases and DNases. The detection assays, which are effective for testing lab reagents, equipment, and other lab supplies, can be read visually for qualitative assessment of contamination or quantified using fluorometry. ORDER CUSTOM PROBE-BASED QPCR ASSAYS 2021 Integrated DNA Technologies, Inc. Trademarks contained herein are the property of Integrated DNA Technologies, Inc. or their respective owners, and may beHOW TO ORDER
Send orders via email using a completed order form to orders@idtdna.com. Request quotes via email using a completed order form to idtquotes@idtdna.com. Tube order form - INC (156 KB) Plate order form - INC (2678 KB) Gene order form - INC (78 KB) RxnReady Order Form (25 KB) Gene Capture Pool and Plate Order Form (25 KB) GMP Tube Order Form (229CUSTOM PRIMERS
The accuracy of design and synthesis of a primer pair is the most important consideration to generate good PCR performance data. Poor design choices, erroneous or truncated sequences, and ineffective purification can lead to unusable results. We have applied thermodynamic and bioinformatic knowledge towards a suite of easy-to-use, online tools to help you design primers.ACCOUNT SETUP
2021 Integrated DNA Technologies, Inc. Trademarks contained herein are the property of Integrated DNA Technologies, Inc. or their respective owners, and may bePRIMERQUEST
Easily design primers or assays for PCR, qPCR, or sequencing (any species). Customization of ~45 parameters, allowing qPCR assay designs: With specific primer, probe, or amplicon criteria. Across a specified location. Design algorithm includes multiple checks to reduce primer-dimer formation. Design algorithm includes multiplechecks to reduce
ULTRAMER DNA OLIGOS
Long, high-quality oligos for demanding applications such as cloning, ddRNAi, homology-directed repair, and gene construction. Ultramer DNA Oligonucleotides are generated by proprietary synthesis methods that deliver high quality oligos up to 200 bases. They are available single- or double-stranded and can be delivered in tubes or plates. LEARN THE TRUTH ABOUT WHAT CRISPR CAN AND CANNOT DO Not too long ago, what is currently possible with CRISPR technology would have been almost unthinkable. The genome editing technology enables fast, precise, intentional, and permanent changes in the DNA of living organisms and cells. RHAMPSEQ DESIGN TOOL rhAmpSeq Design Tool. Deep, targeted amplicon sequencing with highly multiplexed panels for sequencing on Illumina platforms. Generate sequencing panels for a wide range of plant and animal species. Minimize primer dimers and maximize multiplexing capability. Efficiently analyze on- and off-target CRISPR edit sites. GEORGE CHURCH'S WOOLLY MAMMOTH PROJECT A disrupter, a great thinker, Dr. Church envisions the future, embracing an anything-is-possible attitude. In ongoing research, Dr. Church is using IDT oligonucleotides, high-quality, double-stranded DNA, to explore the de-extinction of the woolly mammoth. “We use it for just about every project,” said Dr. Church about IDT’ssynthetic DNA.
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