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polyacrylamide and
DTPCPP, Α,Β NON-HYDROLYZABLE THYMIDINE NUCLEOTIDES For research use only! Shipping: shipped on blue ice Storage Conditions: store at -20 °C Short term exposure (up to 1 week cumulative) to ambient temperature possible. Shelf Life: 12 months after date of delivery Molecular Formula: C 11 H 19 N 2 O 13 P 3 (free acid) Molecular Weight: 480.20 g/mol (free acid) Exact Mass: 480.01 g/mol (free acid) Purity: ≥ 95 % (HPLC) ANTI-DSRNA MONOCLONAL ANTIBODY J2, DSRNA DETECTION SCICONS Anti-dsRNA monoclonal antibody J2 is the gold standard for the detection of dsRNA both in cultured cells and fixed paraffin-embedded histological samples. Applications: ELISA, IF, FACS, IHC, IP, Dot Blot, ChIP, affinity purification, immunoelectron microscopy. Please note that nucleic acid separation prior to dsRNA-immunoblotting mustTAQ POLYMERASE
Taq Polymerase is the enzyme of choice for most routine PCR applications. To allow choosing between convenience and flexibility, Jena Bioscience offers Taq polymerase in four types of formulations ranging from complete master mixes over core kits containing all DAPPNHP, Β,Γ NON-HYDROLYZABLE ADENOSINE NUCLEOTIDES For research use only! Shipping: shipped on blue ice Storage Conditions: store at -20 °C Short term exposure (up to 1 week cumulative) to ambient temperature possible. Shelf Life: 6 months after date of delivery Molecular Formula: C 10 H 17 N 6 O 11 P 3 (free acid) Molecular Weight: 490.20 g/mol (free acid) Exact Mass: 490.02 g/mol (free acid) Purity: ≥ 90 % (HPLC) VZV-GE (AA 48-135), RECOMBINANT VARIZELLA-ZOSTER-VIRUS Background: Varicella-zoster virus (VZV) is an extremely cell-associated alpha herpesvirus. It interacts with cell surface heparan sulfate proteoglycans during virus attachment. VZV-gE is a glycoprotein that plays an active or supportive role in VZV cell membrane fusion. VZV-gE was found to enhance the fusogenic potentialof VZV gB.
CALCIUM CHLORIDE DIHYDRATE For in vitro use only!. Shipping: shipped at ambient temperature Storage Conditions: store at 4 °C Shelf Life: 12 months Molecular Formula: CaCl 2 * 2H 2 O Molecular Weight: 147.01 g/mol CAS#: 10035-04-8 EC number: 233-140-8 Form: liquid Concentration: 5 M PCP-BIOTIN, BIOTINYLATED NUCLEOTIDES FOR RNA LABELING pCp-Biotin is recommended for 3'-End Labeling of RNA/miRNA with T4 RNA Ligase that conjugates a single Biotin molecule to the 3'-OH group of the RNA/miRNA template. The resulting Biotin-labeled RNA/miRNA probes are ideally suited for hybridization experiments such as microarray-based expression profiling or electro mobility shift assays(EMSA).
UNMODIFIED DIDEOXY-NUCLEOSIDE TRIPHOSPHATES (DDNTPS Unmodified dideoxy-Nucleoside triphosphates (ddNTPs) - Bundles. Dideoxynucleotide triphosphates (ddNTPs) lack the 3'-OH group of dNTPs that is essential for polymerase-mediated strand elongation in a PCR. Therefore, ddNTPs are used in combination with a modified Taq polymerase (e.g. JBS Sequencing polymerase or Thermosequenase™) as3'-end
JENA BIOSCIENCENUCLEOTIDES & NUCLEOSIDESCLICK CHEMISTRYMOLECULAR BIOLOGYPROTEINSCRYSTALLOGRAPHY & CRYO-EM Jena Bioscience. » Sign in / Register. Covid-19 Testkit CE IVD and Virus RNA Prep Kit available. After an increased demand due to the Corona Pandemic we can now offer: Viral RNA+DNA Preparation Kit. Complete validated Covid-19 test kit (CE IVD) Mastermixes and raw materials for development of Covid-19 test. DSRNA 142 BP, DSRNA DETECTION SCICONS dsRNA 142 bp is a chemically defined 142 bp long dsRNA sequence intended to be used as a positive control in double-stranded (ds)RNA detection. The sequence does not contain any viral or potentially transforming elements. It is suitable for use as a size standard in dsRNA and RNAi analysis on both non-denaturingpolyacrylamide and
DTPCPP, Α,Β NON-HYDROLYZABLE THYMIDINE NUCLEOTIDES For research use only! Shipping: shipped on blue ice Storage Conditions: store at -20 °C Short term exposure (up to 1 week cumulative) to ambient temperature possible. Shelf Life: 12 months after date of delivery Molecular Formula: C 11 H 19 N 2 O 13 P 3 (free acid) Molecular Weight: 480.20 g/mol (free acid) Exact Mass: 480.01 g/mol (free acid) Purity: ≥ 95 % (HPLC) ANTI-DSRNA MONOCLONAL ANTIBODY J2, DSRNA DETECTION SCICONS Anti-dsRNA monoclonal antibody J2 is the gold standard for the detection of dsRNA both in cultured cells and fixed paraffin-embedded histological samples. Applications: ELISA, IF, FACS, IHC, IP, Dot Blot, ChIP, affinity purification, immunoelectron microscopy. Please note that nucleic acid separation prior to dsRNA-immunoblotting mustTAQ POLYMERASE
Taq Polymerase is the enzyme of choice for most routine PCR applications. To allow choosing between convenience and flexibility, Jena Bioscience offers Taq polymerase in four types of formulations ranging from complete master mixes over core kits containing all DAPPNHP, Β,Γ NON-HYDROLYZABLE ADENOSINE NUCLEOTIDES For research use only! Shipping: shipped on blue ice Storage Conditions: store at -20 °C Short term exposure (up to 1 week cumulative) to ambient temperature possible. Shelf Life: 6 months after date of delivery Molecular Formula: C 10 H 17 N 6 O 11 P 3 (free acid) Molecular Weight: 490.20 g/mol (free acid) Exact Mass: 490.02 g/mol (free acid) Purity: ≥ 90 % (HPLC) VZV-GE (AA 48-135), RECOMBINANT VARIZELLA-ZOSTER-VIRUS Background: Varicella-zoster virus (VZV) is an extremely cell-associated alpha herpesvirus. It interacts with cell surface heparan sulfate proteoglycans during virus attachment. VZV-gE is a glycoprotein that plays an active or supportive role in VZV cell membrane fusion. VZV-gE was found to enhance the fusogenic potentialof VZV gB.
CALCIUM CHLORIDE DIHYDRATE For in vitro use only!. Shipping: shipped at ambient temperature Storage Conditions: store at 4 °C Shelf Life: 12 months Molecular Formula: CaCl 2 * 2H 2 O Molecular Weight: 147.01 g/mol CAS#: 10035-04-8 EC number: 233-140-8 Form: liquid Concentration: 5 M PCP-BIOTIN, BIOTINYLATED NUCLEOTIDES FOR RNA LABELING pCp-Biotin is recommended for 3'-End Labeling of RNA/miRNA with T4 RNA Ligase that conjugates a single Biotin molecule to the 3'-OH group of the RNA/miRNA template. The resulting Biotin-labeled RNA/miRNA probes are ideally suited for hybridization experiments such as microarray-based expression profiling or electro mobility shift assays(EMSA).
UNMODIFIED DIDEOXY-NUCLEOSIDE TRIPHOSPHATES (DDNTPS Unmodified dideoxy-Nucleoside triphosphates (ddNTPs) - Bundles. Dideoxynucleotide triphosphates (ddNTPs) lack the 3'-OH group of dNTPs that is essential for polymerase-mediated strand elongation in a PCR. Therefore, ddNTPs are used in combination with a modified Taq polymerase (e.g. JBS Sequencing polymerase or Thermosequenase™) as3'-end
PROTEIN KINASES
Recombinant Proteins Protein Kinases, Phosphatases and PeptidasesProtein Kinases
VHR, PHOSPHATASES
PR-350 / Recombinant Proteins Protein Kinases, Phosphatases and Peptidases PhosphatasesTHERMOFLUOR SCREENS
The Thermofluor Screens JBScreen FUNDAMENT and JBScreen SPECIFIC allow identification of protein-stabilizing buffer conditions which is pivotal for protein purification, characterization and crystallization . Undesired overlay of screening of interdependent variables is prevented by strictly categorizing stability screening of proteinsinto. 1.
ATPΓS, Γ-PHOSPHATE MODIFIED ADENOSINE NUCLEOTIDES For research use only! Shipping: shipped on dry ice Storage Conditions: store at -20 °C Shelf Life: 6 months after date of delivery Molecular Formula: C 10 H 16 N 5 O 12 P 3 S (free acid) Molecular Weight: 523.24 g/mol (free acid) Exact Mass: 522.97 g/mol (free acid) CAS#: 93839-89-5 Purity: ≥ 90 % (HPLC), contains . 10 % ADP Form: solid Color: white to off-white ATPΑS, Α-PHOSPHATE MODIFIED ADENOSINE NUCLEOTIDES For research use only! Shipping: shipped on blue ice Storage Conditions: store at -20 °C Short term exposure (up to 1 week cumulative) to ambient temperature possible. Shelf Life: 12 months after date of delivery Molecular Formula: C 10 H 16 N 5 O 12 P 3 S (free acid) Molecular Weight: 523.24 g/mol (free acid) Exact Mass: 522.97 g/mol (free acid) CAS#: 29220-54-0 VZV-GE (AA 48-135), RECOMBINANT VARIZELLA-ZOSTER-VIRUS Background: Varicella-zoster virus (VZV) is an extremely cell-associated alpha herpesvirus. It interacts with cell surface heparan sulfate proteoglycans during virus attachment. VZV-gE is a glycoprotein that plays an active or supportive role in VZV cell membrane fusion. VZV-gE was found to enhance the fusogenic potentialof VZV gB.
M27,3'-OGP3G (ARCA CAP ANALOG) For research use only! Shipping: shipped on blue ice Storage Conditions: store at -20 °C Short term exposure (up to 1 week cumulative) to ambient temperature possible. Shelf Life: 12 months after date of delivery Molecular Formula: C 22 H 31 N 10 O 18 P 3 (free acid) Molecular Weight: 816.46 g/mol (free acid) Exact Mass: 816.10 g/mol (free acid) CAS#: 400806-46-4 (acid), 400806-60-2 AU-FLATS™: THE BEST GOLD GRIDS FOR CRYO-EM Au-flat™ is an ultrastable holey gold alloy film (80% Au and 20% Pd) with a thickness of ca. 45 nm supported by a TEM gold grid. Significant improvements last year have made Au-flats™ the new gold standard for Cryo-EM sample supports: Supporting gold grids are ultra-flat with planar surface → resulting in an even ice thickness 2-AMINO-ATP, INTRINSICALLY FLUORESCENT ADENOSINES For research use only! Shipping: shipped on blue ice Storage Conditions: store at -20 °C Short term exposure (up to 1 week cumulative) to ambient temperature possible. Shelf Life: 12 months after date of delivery Molecular Formula: C 10 H 17 N 6 O 13 P 3 (free acid) Molecular Weight: 522.20 g/mol (free acid) Exact Mass: 522.01 g/mol (free acid) CAS#: 18549-34-3 (free acid) TCO-PEG4-NHS ESTER, BIFUNCTIONAL TCO-CONTAINING REAGENTS Immediately before use prepare 10 mM TCO-PEG 4 -NHS reagent in DMSO or DMF. Add a 20-fold molar excess NHS reagent to the protein sample and incubate for 1 hour at room temperature. Stop the reaction by adding Quenching buffer to a final concentration of 50-100 mM, incubate for 5minutes.
JENA BIOSCIENCENUCLEOTIDES & NUCLEOSIDESCLICK CHEMISTRYMOLECULAR BIOLOGYPROTEINSCRYSTALLOGRAPHY & CRYO-EM Jena Bioscience. » Sign in / Register. Covid-19 Testkit CE IVD and Virus RNA Prep Kit available. After an increased demand due to the Corona Pandemic we can now offer: Viral RNA+DNA Preparation Kit. Complete validated Covid-19 test kit (CE IVD) Mastermixes and raw materials for development of Covid-19 test. NUCLEOTIDES & NUCLEOSIDES Most nucleotides are available as small (S) and large (L) packs, with an L pack equal to five S packs. Thus, when ordering an L pack, you may receive five individual S packs. Unless stated otherwise, all our nucleotide preparations are long-term stable when stored at -20°C. If you require large amounts (> 5 L packs) please contact us for DSRNA 142 BP, DSRNA DETECTION SCICONS dsRNA 142 bp is a chemically defined 142 bp long dsRNA sequence intended to be used as a positive control in double-stranded (ds)RNA detection. The sequence does not contain any viral or potentially transforming elements. It is suitable for use as a size standard in dsRNA and RNAi analysis on both non-denaturingpolyacrylamide and
ANTI-DSRNA MONOCLONAL ANTIBODY J2, DSRNA DETECTION SCICONS Anti-dsRNA monoclonal antibody J2 is the gold standard for the detection of dsRNA both in cultured cells and fixed paraffin-embedded histological samples. Applications: ELISA, IF, FACS, IHC, IP, Dot Blot, ChIP, affinity purification, immunoelectron microscopy. Please note that nucleic acid separation prior to dsRNA-immunoblotting mustPROTEIN LABELING
Protein Labeling. The attachment of a reporter group (label) is often required for the detection and biochemical/cellular characterization of proteins and its binding partners. Different labeling techniques & types of label such as fluorophores, biotin and luminescent dyes are available for non-radioactive protein labeling, both random and siteTAQ POLYMERASE
Taq Polymerase is the enzyme of choice for most routine PCR applications. To allow choosing between convenience and flexibility, Jena Bioscience offers Taq polymerase in four types of formulations ranging from complete master mixes over core kits containing all DFHBI-1T, APTAMER-BASED RNA IMAGING & ANALYSIS Form: solid. Color: yellow. Solubility: DMSO. Description: DFHBI-1T is cell-permeable and can be used for in vitro and live cell detection of RNA provided with a DFHBI-1T-specific RNA sequence (DFHBI-1T aptamer) such as Spinach2 or Broccoli. DFHBI-1T possesses a higher photostability than DFHBI which generally results in a higher signal FRAG XTAL SCREEN, FRAGMENT SCREEN The Frag Xtal Screen is designed to directly collect structural data of protein crystals soaked with 96 different fragments. Short Fragment Soaking Protocol: 1) Remove foil carefully. 2) Add 30 μl crystallization buffer to the reservoir (manually or by robot) 3) Add 0.5 μl crystallization buffer on dried fragments (manually or byrobot) 4
DNTP GUIDE - JENA BIOSCIENCE 6 Specifications Building Blocs of Life Test Specification Physical Concentration (22 °C, pH 7.0) 100 – 110 mM Appearance clear colorless solution pH (22 °C) 8.5 ± 0.2 Stability (from certification date) 24 months HPLC dNTP (C18-RP-UV) ≥ 99.0 % (area) IMMOBILIZED Γ-AMINOPHENYL-M7GTP (C10-SPACER), IMMOBILIZED Selected References: Coots et al. (2017) m6A Facilitates eIF4F-Independent mRNA Translation.Mol. Cell. doi:10.1016. Hou et al. (2017) PERK Signal-Modulated Protein Translation Promotes the Survivability of Dengue 2 Virus-Infected Mosquito Cells and Extends Viral Replication Viruses 9(9):262. Theodosakis et al. (2017) p90RSK Blockade Inhibits Dual BRAF and MEK Inhibitor-Resistant Melanoma by JENA BIOSCIENCENUCLEOTIDES & NUCLEOSIDESCLICK CHEMISTRYMOLECULAR BIOLOGYPROTEINSCRYSTALLOGRAPHY & CRYO-EM Jena Bioscience. » Sign in / Register. Covid-19 Testkit CE IVD and Virus RNA Prep Kit available. After an increased demand due to the Corona Pandemic we can now offer: Viral RNA+DNA Preparation Kit. Complete validated Covid-19 test kit (CE IVD) Mastermixes and raw materials for development of Covid-19 test. NUCLEOTIDES & NUCLEOSIDES Most nucleotides are available as small (S) and large (L) packs, with an L pack equal to five S packs. Thus, when ordering an L pack, you may receive five individual S packs. Unless stated otherwise, all our nucleotide preparations are long-term stable when stored at -20°C. If you require large amounts (> 5 L packs) please contact us for DSRNA 142 BP, DSRNA DETECTION SCICONS dsRNA 142 bp is a chemically defined 142 bp long dsRNA sequence intended to be used as a positive control in double-stranded (ds)RNA detection. The sequence does not contain any viral or potentially transforming elements. It is suitable for use as a size standard in dsRNA and RNAi analysis on both non-denaturingpolyacrylamide and
ANTI-DSRNA MONOCLONAL ANTIBODY J2, DSRNA DETECTION SCICONS Anti-dsRNA monoclonal antibody J2 is the gold standard for the detection of dsRNA both in cultured cells and fixed paraffin-embedded histological samples. Applications: ELISA, IF, FACS, IHC, IP, Dot Blot, ChIP, affinity purification, immunoelectron microscopy. Please note that nucleic acid separation prior to dsRNA-immunoblotting mustPROTEIN LABELING
Protein Labeling. The attachment of a reporter group (label) is often required for the detection and biochemical/cellular characterization of proteins and its binding partners. Different labeling techniques & types of label such as fluorophores, biotin and luminescent dyes are available for non-radioactive protein labeling, both random and siteTAQ POLYMERASE
Taq Polymerase is the enzyme of choice for most routine PCR applications. To allow choosing between convenience and flexibility, Jena Bioscience offers Taq polymerase in four types of formulations ranging from complete master mixes over core kits containing all DFHBI-1T, APTAMER-BASED RNA IMAGING & ANALYSIS Form: solid. Color: yellow. Solubility: DMSO. Description: DFHBI-1T is cell-permeable and can be used for in vitro and live cell detection of RNA provided with a DFHBI-1T-specific RNA sequence (DFHBI-1T aptamer) such as Spinach2 or Broccoli. DFHBI-1T possesses a higher photostability than DFHBI which generally results in a higher signal FRAG XTAL SCREEN, FRAGMENT SCREEN The Frag Xtal Screen is designed to directly collect structural data of protein crystals soaked with 96 different fragments. Short Fragment Soaking Protocol: 1) Remove foil carefully. 2) Add 30 μl crystallization buffer to the reservoir (manually or by robot) 3) Add 0.5 μl crystallization buffer on dried fragments (manually or byrobot) 4
DNTP GUIDE - JENA BIOSCIENCE 6 Specifications Building Blocs of Life Test Specification Physical Concentration (22 °C, pH 7.0) 100 – 110 mM Appearance clear colorless solution pH (22 °C) 8.5 ± 0.2 Stability (from certification date) 24 months HPLC dNTP (C18-RP-UV) ≥ 99.0 % (area) IMMOBILIZED Γ-AMINOPHENYL-M7GTP (C10-SPACER), IMMOBILIZED Selected References: Coots et al. (2017) m6A Facilitates eIF4F-Independent mRNA Translation.Mol. Cell. doi:10.1016. Hou et al. (2017) PERK Signal-Modulated Protein Translation Promotes the Survivability of Dengue 2 Virus-Infected Mosquito Cells and Extends Viral Replication Viruses 9(9):262. Theodosakis et al. (2017) p90RSK Blockade Inhibits Dual BRAF and MEK Inhibitor-Resistant Melanoma byCLICK CHEMISTRY
Click Chemistry describes pairs of functional groups that rapidly and selectively react (“click”) with each other under mild, aqueous conditions. The concept of Click Chemistry has been transformed into convenient, versatile and reliable two-step coupling procedures of two molecules A and B that are widely used in biosciences , drug discovery and material science .PROTEIN KINASES
Recombinant Proteins Protein Kinases, Phosphatases and PeptidasesProtein Kinases
INTERNATIONAL DISTRIBUTORS Sapphire Bioscience Pty Ltd. 126 Cope Street Waterloo NSW 2017 Australia Tel.: +61 2 9698 2022 Fax: +61 2 9698 1022 E-Mail: sales@sapphirebioscience.com www.sapphirebioscience.com BIO-LABELING: FAST CLICK OF 3-NLS WITH TETRAZINES Inverse electron demand Diels-Alder additions (IEDDA) between 1,2,4,5-tetrazines and olefins have recently found widespread application as a novel click chemistry scheme .. In particular, the catalyst-free IEDDA reaction between 3-NLS (3-norbornene-L-serine) and 3,6-di-2-pyridyl-1,2,4,5-tetrazine is a versatile alternative to CuAAC (copper-catalysed azide-alkyne cycloaddition) and thiol DFHBI-1T, APTAMER-BASED RNA IMAGING & ANALYSIS Form: solid. Color: yellow. Solubility: DMSO. Description: DFHBI-1T is cell-permeable and can be used for in vitro and live cell detection of RNA provided with a DFHBI-1T-specific RNA sequence (DFHBI-1T aptamer) such as Spinach2 or Broccoli. DFHBI-1T possesses a higher photostability than DFHBI which generally results in a higher signal RESTRICTION ENZYMES: "B" ENZYMES Restriction Enzymes "B" Enzymes. BseCI EN-109 Isoschizomers: BanIII, Bsa29I, Bsp106I, BspDI, BspXI, Bsu15I, BsuTUI, ClaI, ZhoI VZV-GE (AA 48-135), RECOMBINANT VARIZELLA-ZOSTER-VIRUS Background: Varicella-zoster virus (VZV) is an extremely cell-associated alpha herpesvirus. It interacts with cell surface heparan sulfate proteoglycans during virus attachment. VZV-gE is a glycoprotein that plays an active or supportive role in VZV cell membrane fusion. VZV-gE was found to enhance the fusogenic potentialof VZV gB.
CALCIUM CHLORIDE DIHYDRATE For in vitro use only!. Shipping: shipped at ambient temperature Storage Conditions: store at 4 °C Shelf Life: 12 months Molecular Formula: CaCl 2 * 2H 2 O Molecular Weight: 147.01 g/mol CAS#: 10035-04-8 EC number: 233-140-8 Form: liquid Concentration: 5 M MRC 2-WELL AND 3-WELL PLATES MRC 2-well and 3-well Plates. The SWISSCI MRC 2 Well and 3 Well Crystallization Plates are designed in the 96 well plate format with either 2 or 3 protein wells per reservoir well. They were developed in collaboration with experienced protein crystallographers, e.g. the MRC Laboratory of Molecular Biology (Cambridge, UK). DIRECT QPCR PROBESMASTER, DIRECT QPCR / ROBUST PCR-396 / Real-Time PCR / qPCR Direct qPCR / Robust Amplification JENA BIOSCIENCENUCLEOTIDES & NUCLEOSIDESCLICK CHEMISTRYMOLECULAR BIOLOGYPROTEINSCRYSTALLOGRAPHY & CRYO-EM Jena Bioscience. » Sign in / Register. Covid-19 Testkit CE IVD and Virus RNA Prep Kit available. After an increased demand due to the Corona Pandemic we can now offer: Viral RNA+DNA Preparation Kit. Complete validated Covid-19 test kit (CE IVD) Mastermixes and raw materials for development of Covid-19 test. DSRNA 142 BP, DSRNA DETECTION SCICONS dsRNA 142 bp is a chemically defined 142 bp long dsRNA sequence intended to be used as a positive control in double-stranded (ds)RNA detection. The sequence does not contain any viral or potentially transforming elements. It is suitable for use as a size standard in dsRNA and RNAi analysis on both non-denaturingpolyacrylamide and
ATPΓS, Γ-PHOSPHATE MODIFIED ADENOSINE NUCLEOTIDES For research use only! Shipping: shipped on dry ice Storage Conditions: store at -20 °C Shelf Life: 6 months after date of delivery Molecular Formula: C 10 H 16 N 5 O 12 P 3 S (free acid) Molecular Weight: 523.24 g/mol (free acid) Exact Mass: 522.97 g/mol (free acid) CAS#: 93839-89-5 Purity: ≥ 90 % (HPLC), contains . 10 % ADP Form: solid Color: white to off-white ANTI-DSRNA MONOCLONAL ANTIBODY J2, DSRNA DETECTION SCICONS Anti-dsRNA monoclonal antibody J2 is the gold standard for the detection of dsRNA both in cultured cells and fixed paraffin-embedded histological samples. Applications: ELISA, IF, FACS, IHC, IP, Dot Blot, ChIP, affinity purification, immunoelectron microscopy. Please note that nucleic acid separation prior to dsRNA-immunoblotting mustTAQ POLYMERASE
Taq Polymerase is the enzyme of choice for most routine PCR applications. To allow choosing between convenience and flexibility, Jena Bioscience offers Taq polymerase in four types of formulations ranging from complete master mixes over core kits containing all ATPΑS, Α-PHOSPHATE MODIFIED ADENOSINE NUCLEOTIDES For research use only! Shipping: shipped on blue ice Storage Conditions: store at -20 °C Short term exposure (up to 1 week cumulative) to ambient temperature possible. Shelf Life: 12 months after date of delivery Molecular Formula: C 10 H 16 N 5 O 12 P 3 S (free acid) Molecular Weight: 523.24 g/mol (free acid) Exact Mass: 522.97 g/mol (free acid) CAS#: 29220-54-0 FRAG XTAL SCREEN, FRAGMENT SCREEN The Frag Xtal Screen is designed to directly collect structural data of protein crystals soaked with 96 different fragments. Short Fragment Soaking Protocol: 1) Remove foil carefully. 2) Add 30 μl crystallization buffer to the reservoir (manually or by robot) 3) Add 0.5 μl crystallization buffer on dried fragments (manually or byrobot) 4
DNTP GUIDE - JENA BIOSCIENCE 6 Specifications Building Blocs of Life Test Specification Physical Concentration (22 °C, pH 7.0) 100 – 110 mM Appearance clear colorless solution pH (22 °C) 8.5 ± 0.2 Stability (from certification date) 24 months HPLC dNTP (C18-RP-UV) ≥ 99.0 % (area) ADA - 1 M, CRYSTALLIZATION STOCK SOLUTIONS: BUFFERS - JENA For in vitro use only!. Shipping: shipped at ambient temperature Storage Conditions: store at 4 °C Shelf Life: 12 months Molecular Formula: H 2 NCOCH 2 N(CH 2 CO 2 H) 2 Molecular Weight: 190.15 g/mol CAS#: 26239-55-4 EC number: 247-530-0 Form: liquid Concentration: 1 M Description: Crystallization stock solution, sterile filtered: 1 M ADA adjusted to any pH value within the buffer range pH 6 UNMODIFIED DIDEOXY-NUCLEOSIDE TRIPHOSPHATES (DDNTPS Unmodified dideoxy-Nucleoside triphosphates (ddNTPs) - Bundles. Dideoxynucleotide triphosphates (ddNTPs) lack the 3'-OH group of dNTPs that is essential for polymerase-mediated strand elongation in a PCR. Therefore, ddNTPs are used in combination with a modified Taq polymerase (e.g. JBS Sequencing polymerase or Thermosequenase™) as3'-end
JENA BIOSCIENCENUCLEOTIDES & NUCLEOSIDESCLICK CHEMISTRYMOLECULAR BIOLOGYPROTEINSCRYSTALLOGRAPHY & CRYO-EM Jena Bioscience. » Sign in / Register. Covid-19 Testkit CE IVD and Virus RNA Prep Kit available. After an increased demand due to the Corona Pandemic we can now offer: Viral RNA+DNA Preparation Kit. Complete validated Covid-19 test kit (CE IVD) Mastermixes and raw materials for development of Covid-19 test. DSRNA 142 BP, DSRNA DETECTION SCICONS dsRNA 142 bp is a chemically defined 142 bp long dsRNA sequence intended to be used as a positive control in double-stranded (ds)RNA detection. The sequence does not contain any viral or potentially transforming elements. It is suitable for use as a size standard in dsRNA and RNAi analysis on both non-denaturingpolyacrylamide and
ATPΓS, Γ-PHOSPHATE MODIFIED ADENOSINE NUCLEOTIDES For research use only! Shipping: shipped on dry ice Storage Conditions: store at -20 °C Shelf Life: 6 months after date of delivery Molecular Formula: C 10 H 16 N 5 O 12 P 3 S (free acid) Molecular Weight: 523.24 g/mol (free acid) Exact Mass: 522.97 g/mol (free acid) CAS#: 93839-89-5 Purity: ≥ 90 % (HPLC), contains . 10 % ADP Form: solid Color: white to off-white ANTI-DSRNA MONOCLONAL ANTIBODY J2, DSRNA DETECTION SCICONS Anti-dsRNA monoclonal antibody J2 is the gold standard for the detection of dsRNA both in cultured cells and fixed paraffin-embedded histological samples. Applications: ELISA, IF, FACS, IHC, IP, Dot Blot, ChIP, affinity purification, immunoelectron microscopy. Please note that nucleic acid separation prior to dsRNA-immunoblotting mustTAQ POLYMERASE
Taq Polymerase is the enzyme of choice for most routine PCR applications. To allow choosing between convenience and flexibility, Jena Bioscience offers Taq polymerase in four types of formulations ranging from complete master mixes over core kits containing all ATPΑS, Α-PHOSPHATE MODIFIED ADENOSINE NUCLEOTIDES For research use only! Shipping: shipped on blue ice Storage Conditions: store at -20 °C Short term exposure (up to 1 week cumulative) to ambient temperature possible. Shelf Life: 12 months after date of delivery Molecular Formula: C 10 H 16 N 5 O 12 P 3 S (free acid) Molecular Weight: 523.24 g/mol (free acid) Exact Mass: 522.97 g/mol (free acid) CAS#: 29220-54-0 FRAG XTAL SCREEN, FRAGMENT SCREEN The Frag Xtal Screen is designed to directly collect structural data of protein crystals soaked with 96 different fragments. Short Fragment Soaking Protocol: 1) Remove foil carefully. 2) Add 30 μl crystallization buffer to the reservoir (manually or by robot) 3) Add 0.5 μl crystallization buffer on dried fragments (manually or byrobot) 4
DNTP GUIDE - JENA BIOSCIENCE 6 Specifications Building Blocs of Life Test Specification Physical Concentration (22 °C, pH 7.0) 100 – 110 mM Appearance clear colorless solution pH (22 °C) 8.5 ± 0.2 Stability (from certification date) 24 months HPLC dNTP (C18-RP-UV) ≥ 99.0 % (area) ADA - 1 M, CRYSTALLIZATION STOCK SOLUTIONS: BUFFERS - JENA For in vitro use only!. Shipping: shipped at ambient temperature Storage Conditions: store at 4 °C Shelf Life: 12 months Molecular Formula: H 2 NCOCH 2 N(CH 2 CO 2 H) 2 Molecular Weight: 190.15 g/mol CAS#: 26239-55-4 EC number: 247-530-0 Form: liquid Concentration: 1 M Description: Crystallization stock solution, sterile filtered: 1 M ADA adjusted to any pH value within the buffer range pH 6 UNMODIFIED DIDEOXY-NUCLEOSIDE TRIPHOSPHATES (DDNTPS Unmodified dideoxy-Nucleoside triphosphates (ddNTPs) - Bundles. Dideoxynucleotide triphosphates (ddNTPs) lack the 3'-OH group of dNTPs that is essential for polymerase-mediated strand elongation in a PCR. Therefore, ddNTPs are used in combination with a modified Taq polymerase (e.g. JBS Sequencing polymerase or Thermosequenase™) as3'-end
PROTEIN KINASES
Recombinant Proteins Protein Kinases, Phosphatases and PeptidasesProtein Kinases
ATPΓS, Γ-PHOSPHATE MODIFIED ADENOSINE NUCLEOTIDES Selected References: Buzzi et al. (2009) Extracellular ATP activates MAP kinase cascades through a P2Y purinergic receptor in the human intestinal Caco-2 cell line.Biochim. Biophys. Acta 1790:1651. Wu et al. (2008) Extracellular ATP-induced NO production and ist dependence on membrane Ca 2+ flux in Salvia miltiorrhiza hairy roots. J. Experim. Botany 5 BIO-LABELING: FAST CLICK OF 3-NLS WITH TETRAZINES Inverse electron demand Diels-Alder additions (IEDDA) between 1,2,4,5-tetrazines and olefins have recently found widespread application as a novel click chemistry scheme .. In particular, the catalyst-free IEDDA reaction between 3-NLS (3-norbornene-L-serine) and 3,6-di-2-pyridyl-1,2,4,5-tetrazine is a versatile alternative to CuAAC (copper-catalysed azide-alkyne cycloaddition) and thiol ATPΑS, Α-PHOSPHATE MODIFIED ADENOSINE NUCLEOTIDES For research use only! Shipping: shipped on blue ice Storage Conditions: store at -20 °C Short term exposure (up to 1 week cumulative) to ambient temperature possible. Shelf Life: 12 months after date of delivery Molecular Formula: C 10 H 16 N 5 O 12 P 3 S (free acid) Molecular Weight: 523.24 g/mol (free acid) Exact Mass: 522.97 g/mol (free acid) CAS#: 29220-54-0 CRYSTALLIZATION STOCK SOLUTIONS: BUFFERS Crystallization stock solutions, i.e. polymers, buffers and salts are ideal for the optimization of your crystallization conditions. Using the same chemicals as utilized in the JBScreens ensures higher reproducibility of your experiments. Crystallization Stock Solutions are ready for use: the concentration is adjusted and they are sterilefiltered.
LEXSY CLONING AND EXPRESSION FAQS Q2: Can the target gene be introduced into L. tarentolae on a circular plasmid? A2: Yes, circular plasmids will be maintained in L. tarentolae cells extrachromosomally. For high-yield protein expression from episomal constructs we developed the 4th generation of inducible LEXSY (EGE-1420). However, if integrative expression plasmids are transfected in circular form, the yields of expressed UNMODIFIED DIDEOXY-NUCLEOSIDE TRIPHOSPHATES (DDNTPS Figure 2: The single basepair extension (SBE) assay is a reliable method for the detection of a priori known locations of single nucleotide polymorphisms (SNPs) .It relies on the extension of a primer, designed to bind one nucleotide upstream of the polymorphic spot, by a matching ddNTP. Subsequent detection of the incorporated ddNTP is performed by mass spectrometry of the extended primer 2-AMINO-ATP, INTRINSICALLY FLUORESCENT ADENOSINES For research use only! Shipping: shipped on blue ice Storage Conditions: store at -20 °C Short term exposure (up to 1 week cumulative) to ambient temperature possible. Shelf Life: 12 months after date of delivery Molecular Formula: C 10 H 17 N 6 O 13 P 3 (free acid) Molecular Weight: 522.20 g/mol (free acid) Exact Mass: 522.01 g/mol (free acid) CAS#: 18549-34-3 (free acid) OLIGO (DT)20, RANDOM AND OLIGO (DT) PRIMERS For in vitro use only!. Shipping: shipped on blue ice Storage Conditions: store at -20 °C avoid freeze/thaw cycles Shelf Life: 12 months Molecular Weight: 6022 g/mol Purity: by HPLC Form: clear aqueous solution Concentration: 100 μM Description: Oligo (dT) Primer hybridizes to the poly(A) tail of mRNA and is used as primer for first stand cDNA synthesis with reverse transcriptases. DIRECT QPCR PROBESMASTER, DIRECT QPCR / ROBUST PCR-396 / Real-Time PCR / qPCR Direct qPCR / Robust Amplification Sign in / Register Need a quotation? Create it here! No login/account required. YOUR BASKET/ONLINE QUOTEItems: 0 (0,00 €)
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New Pathways to DNA Amplification!Imagine...
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Non-specific elimination of nucleic acids of protein samples Also Available in bulk formatAug 19, 2019
Interested in ARCA-capped and internally modified RNA? We now offer HighYield kits for synthesis of ARCA-capped and/or 5-methylcytosine & pseudouridine-modified (m)RNA.Aug 12, 2019
Biotin-16-ddUTP
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a clickable probe for the detection of protein ADP-ribosylation, is now back in our catalog.more news ...
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