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NIKON’S MUSEUM OF MICROSCOPY Nikon’s Museum of Microscopy. Nikon has been producing microscopes for almost a century, starting with a simple brass compound monocular brass microscope in the early 1900s. Today, Nikon microscopes are among the best in the World and are found in research laboratories in almost every country. The Nikon Museum of Microscopy features many of DEPTH OF FIELD CALCULATOR The total depth of field is given by the sum of the wave and geometrical optical depths of fields as: Formula 1 - Total Depth of Field. d t o t = λ ⋅ n N A 2 + n M ⋅ N A e. Where d (tot) represents the depth of field, l is the wavelength of illuminating light, n is the refractive index of the medium (usually air (1.000) orimmersion oil (1
ECLIPSE E400
Eclipse E400. ( 1997 ) The Eclipse E400 biological microscope is Nikon's mid-level research instrument from the late 1990s. Though designed for stability, the instrument features a compact design, which was intended to help maintain a suitable comfort level for the microscopist over extended periods of LABOPHOT ENTRY-LEVEL RESEARCH MICROSCOPE Labophot Entry-Level Research Microscope. The Labophot was a sturdy, versatile microscope, produced in the 1980s. It offered research-level performance and a modular system that made it highly adaptable for clinical, biological, industrial, and educational laboratories. The Labophot utilized the Nikon CF optical system, which yielded high MATCHING CAMERA TO MICROSCOPE The ultimate resolution of a charge-coupled device (CCD) or complementary metal oxide semiconductor (CMOS) image sensor is a function of the number of photodiodes and their size relative to the image projected onto the surface of the imaging array by the microscope optical system.When attempting to match microscope optical resolution to a specific digital camera and video coupler combination THE NIKON PERFECT FOCUS SYSTEM (PFS) The Nikon Perfect Focus System (PFS) Nikon has addressed the problem of focus drift by creating a unique hardware solution, termed the Perfect Focus System ( PFS ), which is designed to combat axial focus fluctuations in real time during long-term imaging investigations. A wide range of dry, water, and oil immersion objectives, from 4x to100x
DUAL BAND EXCITATION: DAPI-FITC The DAPI-FITC dual band fluorescence filter combination is designed specifically for simultaneous detection of the fluorochromes DAPI and FITC with minimal crosstalk between bands, and can be employed with other pairs of fluorescent probes having similar spectral profiles.The shorter-wavelength signal channel of the filter set selects narrowly defined spectral regions for violet excitation and ADJUSTMENT OF OBJECTIVE CORRECTION COLLARS The tutorial initializes with a randomly selected specimen appearing in the Microscope Image window and a cut-away view of a correction collar objective positioned to the right of the image window. Also, upon initialization, the tutorial automatically selects a random cover glass thickness for the specimen being viewed in the Microscope Image window. . In order to operate the tutorial, use the YELLOW FLUORESCENT PROTEIN (YFP) EXCITATION NIKON’S MICROSCOPYU The creator of MicroscopyU, Davidson also authored many scientific articles on the subject of photomicrography. His photomicrographs were published in more than a thousand national and international scientific journals, popular magazines and newspapers. In addition, Davidson’s photomicrography won more than 40 awards in scientificand
NIKON’S MUSEUM OF MICROSCOPY Nikon’s Museum of Microscopy. Nikon has been producing microscopes for almost a century, starting with a simple brass compound monocular brass microscope in the early 1900s. Today, Nikon microscopes are among the best in the World and are found in research laboratories in almost every country. The Nikon Museum of Microscopy features many of DEPTH OF FIELD CALCULATOR The total depth of field is given by the sum of the wave and geometrical optical depths of fields as: Formula 1 - Total Depth of Field. d t o t = λ ⋅ n N A 2 + n M ⋅ N A e. Where d (tot) represents the depth of field, l is the wavelength of illuminating light, n is the refractive index of the medium (usually air (1.000) orimmersion oil (1
ECLIPSE E400
Eclipse E400. ( 1997 ) The Eclipse E400 biological microscope is Nikon's mid-level research instrument from the late 1990s. Though designed for stability, the instrument features a compact design, which was intended to help maintain a suitable comfort level for the microscopist over extended periods of LABOPHOT ENTRY-LEVEL RESEARCH MICROSCOPE Labophot Entry-Level Research Microscope. The Labophot was a sturdy, versatile microscope, produced in the 1980s. It offered research-level performance and a modular system that made it highly adaptable for clinical, biological, industrial, and educational laboratories. The Labophot utilized the Nikon CF optical system, which yielded high MATCHING CAMERA TO MICROSCOPE The ultimate resolution of a charge-coupled device (CCD) or complementary metal oxide semiconductor (CMOS) image sensor is a function of the number of photodiodes and their size relative to the image projected onto the surface of the imaging array by the microscope optical system.When attempting to match microscope optical resolution to a specific digital camera and video coupler combination THE NIKON PERFECT FOCUS SYSTEM (PFS) The Nikon Perfect Focus System (PFS) Nikon has addressed the problem of focus drift by creating a unique hardware solution, termed the Perfect Focus System ( PFS ), which is designed to combat axial focus fluctuations in real time during long-term imaging investigations. A wide range of dry, water, and oil immersion objectives, from 4x to100x
DUAL BAND EXCITATION: DAPI-FITC The DAPI-FITC dual band fluorescence filter combination is designed specifically for simultaneous detection of the fluorochromes DAPI and FITC with minimal crosstalk between bands, and can be employed with other pairs of fluorescent probes having similar spectral profiles.The shorter-wavelength signal channel of the filter set selects narrowly defined spectral regions for violet excitation and ADJUSTMENT OF OBJECTIVE CORRECTION COLLARS The tutorial initializes with a randomly selected specimen appearing in the Microscope Image window and a cut-away view of a correction collar objective positioned to the right of the image window. Also, upon initialization, the tutorial automatically selects a random cover glass thickness for the specimen being viewed in the Microscope Image window. . In order to operate the tutorial, use the YELLOW FLUORESCENT PROTEIN (YFP) EXCITATION DIC | NIKON’S MICROSCOPYU An excellent mechanism for rendering contrast in transparent specimens, differential interference contrast (DIC) microscopy is a beam-shearing interference system in which the reference beam is sheared by a minuscule amount, generally somewhat less than the diameter of an Airy disk.The technique produces a monochromatic shadow-cast image that effectively displays the gradient of opticalpaths
FLUORESCENCE
Fluorescence Microscopy. In the rapidly expanding fields of cellular and molecular biology, widefield and confocal fluorescence illumination and observation is becoming one of the techniques of choice. These techniques, which are almost universally employed in both the medical and biological sciences, have spurred the developmentof more
INFINITY OPTICAL SYSTEMS Infinity-corrected microscopes, now a standard in the industry, allow introduction of auxiliary components, such as differential interference contrast (DIC) prisms, polarizers, and epi-fluorescence illuminators, into the parallel optical path between the objective and the tube lens with only a minimal effect on focus and aberrationcorrections.
INNOVATIONS IN LIGHT MICROSCOPY Innovations in Light Microscopy. Of all scientific instruments, probably none has had more thought and labor devoted to its improvement than the light microscope. Its evolution over the past centuries has been driven by scientists who wish to observe and measure phenomena that were smaller, fainter, ( Figure 1) and deeperinside tissue than
RESOLUTION | NIKON’S MICROSCOPYU Resolution (r) = λ/ (2NA) Formula 2 - Numerical Aperture, Wavelength, and Resolution. Resolution (r) = 0.61λ/NA. Formula 3 - Numerical Aperture, Wavelength, and Resolution. Resolution (r) = 1.22λ/ (NA (obj) + NA (cond)) Where r is resolution (the smallest resolvable distance between two objects), NA is a general term for the microscope ECLIPSE TE2000 INVERTED MICROSCOPE In early 2000, Nikon introduced their flagship inverted microscope system, the TE2000. This was the first in the now world-famous Eclipse inverted series of microscopes, which took advantage of the breakthrough CFI60 Infinity Optical System. The TE2000 was developed as a full system with 3 models, the S, the U and the E.ECLIPSE TS100
The Eclipse TS100 inverted microscope was introduced in 1999 to replace the wildly successful Nikon TMS microscope, which had been an industry staple for 17 years. The TS100 was a new generation microscope designed to incorporate the then new infinity optical system which allowed modules to be placed between the eyepiece and theobjectives
MODEL S MICROSCOPE
Model S Microscope. ( Circa late 1960s ) Interchangeable parts made the Model S microscope a versatile tool for studies in biology, medicine, and metallurgy during the 1960s and 1970s. Depending on the user's needs, the microscope could be equipped with various combinations of eyepieces, eyepiece tubes, objectives, condensers, andstages. Nikon
INTRODUCTION TO POLARIZED LIGHT Introduction to Polarized Light. Sunlight and almost every other form of natural and artificial illumination produces light waves whose electric field vectors vibrate in all planes that are perpendicular with respect to the direction of propagation. If the electric field vectors are restricted to MODULATION TRANSFER FUNCTION The resolution and performance of an optical microscope can be characterized by a quantity known as the modulation transfer function (MTF), which is a measurement of the microscope's ability to transfer contrast from the specimen to the intermediate image plane at a specific resolution. Computation of the modulation transfer functionis a
NIKON’S MICROSCOPYU The creator of MicroscopyU, Davidson also authored many scientific articles on the subject of photomicrography. His photomicrographs were published in more than a thousand national and international scientific journals, popular magazines and newspapers. In addition, Davidson’s photomicrography won more than 40 awards in scientificand
ECLIPSE TS100
The Eclipse TS100 inverted microscope was introduced in 1999 to replace the wildly successful Nikon TMS microscope, which had been an industry staple for 17 years. The TS100 was a new generation microscope designed to incorporate the then new infinity optical system which allowed modules to be placed between the eyepiece and theobjectives
MODEL S MICROSCOPE
Model S Microscope. ( Circa late 1960s ) Interchangeable parts made the Model S microscope a versatile tool for studies in biology, medicine, and metallurgy during the 1960s and 1970s. Depending on the user's needs, the microscope could be equipped with various combinations of eyepieces, eyepiece tubes, objectives, condensers, andstages. Nikon
ECLIPSE TE2000 INVERTED MICROSCOPE In early 2000, Nikon introduced their flagship inverted microscope system, the TE2000. This was the first in the now world-famous Eclipse inverted series of microscopes, which took advantage of the breakthrough CFI60 Infinity Optical System. The TE2000 was developed as a full system with 3 models, the S, the U and the E.SMZ STEROMICROSCOPE
SMZ Steromicroscope. The SMZ series are the flagship system of stereomicroscopes offered by Nikon beginning in 1966. Spanning over many decades, this classic series of scopes have ushered in a new generation of stereomicroscopy with cutting edge optics, longer zoom capabilities and lower signal-to-noise ratios than ever previouslyoffered.
ECLIPSE E600
Nikon's Eclipse E600 research microscope is equipped with the revolutionary CFI 60 infinity optical system, providing bright, sharp, crisp and clear images in all applications. Available since the mid-1990s, the mid-tier microscope incorporates completely new specifications adopted for the CFI 60 series objectives, including a60-millimeter
ECLIPSE E400
Eclipse E400. ( 1997 ) The Eclipse E400 biological microscope is Nikon's mid-level research instrument from the late 1990s. Though designed for stability, the instrument features a compact design, which was intended to help maintain a suitable comfort level for the microscopist over extended periods of THE NIKON PERFECT FOCUS SYSTEM (PFS) The Nikon Perfect Focus System (PFS) Nikon has addressed the problem of focus drift by creating a unique hardware solution, termed the Perfect Focus System ( PFS ), which is designed to combat axial focus fluctuations in real time during long-term imaging investigations. A wide range of dry, water, and oil immersion objectives, from 4x to100x
CHAOS CAROLINENSIS (AMOEBA) Chaos carolinensis (Amoeba) Many species that belong to the order Amoebida are free living, but some are well-known parasites of plants and animals. At least six forms of amoeba are parasitic in humans The most well known of these is Entamoeba histolytica, which causes amebicdysentery.
MODEL SMZ-2T STEREOMICROSCOPE Model SMZ-2T Stereomicroscope. ( Circa 1987 ) The Nikon SMZ-2T microscope is a trinocular stereoscopic model that was designed to easily accept a closed-circuit television (CCTV) or newer CCD-based digital camera or any of Nikon's FX photomicrographic systems. A switchover control ring in the trinocular head allows the microscopistto rapidly
NIKON’S MICROSCOPYU The creator of MicroscopyU, Davidson also authored many scientific articles on the subject of photomicrography. His photomicrographs were published in more than a thousand national and international scientific journals, popular magazines and newspapers. In addition, Davidson’s photomicrography won more than 40 awards in scientificand
ECLIPSE TS100
The Eclipse TS100 inverted microscope was introduced in 1999 to replace the wildly successful Nikon TMS microscope, which had been an industry staple for 17 years. The TS100 was a new generation microscope designed to incorporate the then new infinity optical system which allowed modules to be placed between the eyepiece and theobjectives
MODEL S MICROSCOPE
Model S Microscope. ( Circa late 1960s ) Interchangeable parts made the Model S microscope a versatile tool for studies in biology, medicine, and metallurgy during the 1960s and 1970s. Depending on the user's needs, the microscope could be equipped with various combinations of eyepieces, eyepiece tubes, objectives, condensers, andstages. Nikon
ECLIPSE TE2000 INVERTED MICROSCOPE In early 2000, Nikon introduced their flagship inverted microscope system, the TE2000. This was the first in the now world-famous Eclipse inverted series of microscopes, which took advantage of the breakthrough CFI60 Infinity Optical System. The TE2000 was developed as a full system with 3 models, the S, the U and the E.SMZ STEROMICROSCOPE
SMZ Steromicroscope. The SMZ series are the flagship system of stereomicroscopes offered by Nikon beginning in 1966. Spanning over many decades, this classic series of scopes have ushered in a new generation of stereomicroscopy with cutting edge optics, longer zoom capabilities and lower signal-to-noise ratios than ever previouslyoffered.
ECLIPSE E600
Nikon's Eclipse E600 research microscope is equipped with the revolutionary CFI 60 infinity optical system, providing bright, sharp, crisp and clear images in all applications. Available since the mid-1990s, the mid-tier microscope incorporates completely new specifications adopted for the CFI 60 series objectives, including a60-millimeter
ECLIPSE E400
Eclipse E400. ( 1997 ) The Eclipse E400 biological microscope is Nikon's mid-level research instrument from the late 1990s. Though designed for stability, the instrument features a compact design, which was intended to help maintain a suitable comfort level for the microscopist over extended periods of THE NIKON PERFECT FOCUS SYSTEM (PFS) The Nikon Perfect Focus System (PFS) Nikon has addressed the problem of focus drift by creating a unique hardware solution, termed the Perfect Focus System ( PFS ), which is designed to combat axial focus fluctuations in real time during long-term imaging investigations. A wide range of dry, water, and oil immersion objectives, from 4x to100x
CHAOS CAROLINENSIS (AMOEBA) Chaos carolinensis (Amoeba) Many species that belong to the order Amoebida are free living, but some are well-known parasites of plants and animals. At least six forms of amoeba are parasitic in humans The most well known of these is Entamoeba histolytica, which causes amebicdysentery.
MODEL SMZ-2T STEREOMICROSCOPE Model SMZ-2T Stereomicroscope. ( Circa 1987 ) The Nikon SMZ-2T microscope is a trinocular stereoscopic model that was designed to easily accept a closed-circuit television (CCTV) or newer CCD-based digital camera or any of Nikon's FX photomicrographic systems. A switchover control ring in the trinocular head allows the microscopistto rapidly
NIKON’S MUSEUM OF MICROSCOPY Nikon’s Museum of Microscopy. Nikon has been producing microscopes for almost a century, starting with a simple brass compound monocular brass microscope in the early 1900s. Today, Nikon microscopes are among the best in the World and are found in research laboratories in almost every country. The Nikon Museum of Microscopy features many of LIVE-CELL IMAGING CULTURE CHAMBERS A variety of Petri dish configurations specifically designed for high-resolution live-cell imaging are commercially available (see Figure 4). The simplest and perhaps most versatile design consists of a standard 35 or 50-millimeter disposable Petri dish featuring a circular opening (10, 14, or 20 millimeters) cut into the center of the attachment surface and covered by a high-qualityECLIPSE 80I
Eclipse 80i Upright Microscope. In response to Nikon’s ever-growing prominence in the cutting-edge research microscopy markets, Nikon introduced its all-new lineup of microscopes, the i-series. The 80i was one of the first in a family of very commercially successful upright microscopes that would help define the growth of the digitalera for
ECLIPSE E400
Eclipse E400. ( 1997 ) The Eclipse E400 biological microscope is Nikon's mid-level research instrument from the late 1990s. Though designed for stability, the instrument features a compact design, which was intended to help maintain a suitable comfort level for the microscopist over extended periods of NIKON PERFECT FOCUS SYSTEM (PFS) Nikon has addressed the problem of focus drift by creating a unique hardware solution, termed the Perfect Focus System (PFS), which is designed to combat axial focus fluctuations in real time during long-term imaging investigations.A wide range of dry, water, and oil immersion objectives, from 4x to 100x with varying numerical apertures and working distances, can be used with the TiE-PFS ADJUSTMENT OF OBJECTIVE CORRECTION COLLARS The tutorial initializes with a randomly selected specimen appearing in the Microscope Image window and a cut-away view of a correction collar objective positioned to the right of the image window. Also, upon initialization, the tutorial automatically selects a random cover glass thickness for the specimen being viewed in the Microscope Image window. . In order to operate the tutorial, use the LABOPHOT ENTRY-LEVEL RESEARCH MICROSCOPE Labophot Entry-Level Research Microscope. The Labophot was a sturdy, versatile microscope, produced in the 1980s. It offered research-level performance and a modular system that made it highly adaptable for clinical, biological, industrial, and educational laboratories. The Labophot utilized the Nikon CF optical system, which yielded highCCD NOISE SOURCES
CCD Noise Sources. Dark noise and read-out noise are two major sources of noise in CCD and other digital cameras for microscopy. Although great improvements have been made over the past few years in the reduction of CCD dark noise at room temperature, cooling the chip further reduces the noise tenfold per 20° C decrease.ECLIPSE 50/55I
Eclipse 50/55i. The Nikon Eclipse 50/55i was an important milestone in the evolution of Nikon’s clinical line of microscopes. Introduced in 2003, this microscope was a major step in incorporating a more comfortable, user-based design to allow people to sit and use the equipment for long hours without experiencing excessive discomfort orfatigue.
WWW.MICROSCOPYU.COM
Moved Permanently. The document has moved here. NIKON’S MICROSCOPYU The creator of MicroscopyU, Davidson also authored many scientific articles on the subject of photomicrography. His photomicrographs were published in more than a thousand national and international scientific journals, popular magazines and newspapers. In addition, Davidson’s photomicrography won more than 40 awards in scientificand
ECLIPSE TS100
The Eclipse TS100 inverted microscope was introduced in 1999 to replace the wildly successful Nikon TMS microscope, which had been an industry staple for 17 years. The TS100 was a new generation microscope designed to incorporate the then new infinity optical system which allowed modules to be placed between the eyepiece and theobjectives
MODEL S MICROSCOPE
Model S Microscope. ( Circa late 1960s ) Interchangeable parts made the Model S microscope a versatile tool for studies in biology, medicine, and metallurgy during the 1960s and 1970s. Depending on the user's needs, the microscope could be equipped with various combinations of eyepieces, eyepiece tubes, objectives, condensers, andstages. Nikon
ECLIPSE TE2000 INVERTED MICROSCOPE In early 2000, Nikon introduced their flagship inverted microscope system, the TE2000. This was the first in the now world-famous Eclipse inverted series of microscopes, which took advantage of the breakthrough CFI60 Infinity Optical System. The TE2000 was developed as a full system with 3 models, the S, the U and the E.SMZ STEROMICROSCOPE
SMZ Steromicroscope. The SMZ series are the flagship system of stereomicroscopes offered by Nikon beginning in 1966. Spanning over many decades, this classic series of scopes have ushered in a new generation of stereomicroscopy with cutting edge optics, longer zoom capabilities and lower signal-to-noise ratios than ever previouslyoffered.
ECLIPSE E600
Nikon's Eclipse E600 research microscope is equipped with the revolutionary CFI 60 infinity optical system, providing bright, sharp, crisp and clear images in all applications. Available since the mid-1990s, the mid-tier microscope incorporates completely new specifications adopted for the CFI 60 series objectives, including a60-millimeter
ECLIPSE E400
Eclipse E400. ( 1997 ) The Eclipse E400 biological microscope is Nikon's mid-level research instrument from the late 1990s. Though designed for stability, the instrument features a compact design, which was intended to help maintain a suitable comfort level for the microscopist over extended periods of THE NIKON PERFECT FOCUS SYSTEM (PFS) The Nikon Perfect Focus System (PFS) Nikon has addressed the problem of focus drift by creating a unique hardware solution, termed the Perfect Focus System ( PFS ), which is designed to combat axial focus fluctuations in real time during long-term imaging investigations. A wide range of dry, water, and oil immersion objectives, from 4x to100x
CHAOS CAROLINENSIS (AMOEBA) Chaos carolinensis (Amoeba) Many species that belong to the order Amoebida are free living, but some are well-known parasites of plants and animals. At least six forms of amoeba are parasitic in humans The most well known of these is Entamoeba histolytica, which causes amebicdysentery.
MODEL SMZ-2T STEREOMICROSCOPE Model SMZ-2T Stereomicroscope. ( Circa 1987 ) The Nikon SMZ-2T microscope is a trinocular stereoscopic model that was designed to easily accept a closed-circuit television (CCTV) or newer CCD-based digital camera or any of Nikon's FX photomicrographic systems. A switchover control ring in the trinocular head allows the microscopistto rapidly
NIKON’S MICROSCOPYU The creator of MicroscopyU, Davidson also authored many scientific articles on the subject of photomicrography. His photomicrographs were published in more than a thousand national and international scientific journals, popular magazines and newspapers. In addition, Davidson’s photomicrography won more than 40 awards in scientificand
ECLIPSE TS100
The Eclipse TS100 inverted microscope was introduced in 1999 to replace the wildly successful Nikon TMS microscope, which had been an industry staple for 17 years. The TS100 was a new generation microscope designed to incorporate the then new infinity optical system which allowed modules to be placed between the eyepiece and theobjectives
MODEL S MICROSCOPE
Model S Microscope. ( Circa late 1960s ) Interchangeable parts made the Model S microscope a versatile tool for studies in biology, medicine, and metallurgy during the 1960s and 1970s. Depending on the user's needs, the microscope could be equipped with various combinations of eyepieces, eyepiece tubes, objectives, condensers, andstages. Nikon
ECLIPSE TE2000 INVERTED MICROSCOPE In early 2000, Nikon introduced their flagship inverted microscope system, the TE2000. This was the first in the now world-famous Eclipse inverted series of microscopes, which took advantage of the breakthrough CFI60 Infinity Optical System. The TE2000 was developed as a full system with 3 models, the S, the U and the E.SMZ STEROMICROSCOPE
SMZ Steromicroscope. The SMZ series are the flagship system of stereomicroscopes offered by Nikon beginning in 1966. Spanning over many decades, this classic series of scopes have ushered in a new generation of stereomicroscopy with cutting edge optics, longer zoom capabilities and lower signal-to-noise ratios than ever previouslyoffered.
ECLIPSE E600
Nikon's Eclipse E600 research microscope is equipped with the revolutionary CFI 60 infinity optical system, providing bright, sharp, crisp and clear images in all applications. Available since the mid-1990s, the mid-tier microscope incorporates completely new specifications adopted for the CFI 60 series objectives, including a60-millimeter
ECLIPSE E400
Eclipse E400. ( 1997 ) The Eclipse E400 biological microscope is Nikon's mid-level research instrument from the late 1990s. Though designed for stability, the instrument features a compact design, which was intended to help maintain a suitable comfort level for the microscopist over extended periods of THE NIKON PERFECT FOCUS SYSTEM (PFS) The Nikon Perfect Focus System (PFS) Nikon has addressed the problem of focus drift by creating a unique hardware solution, termed the Perfect Focus System ( PFS ), which is designed to combat axial focus fluctuations in real time during long-term imaging investigations. A wide range of dry, water, and oil immersion objectives, from 4x to100x
CHAOS CAROLINENSIS (AMOEBA) Chaos carolinensis (Amoeba) Many species that belong to the order Amoebida are free living, but some are well-known parasites of plants and animals. At least six forms of amoeba are parasitic in humans The most well known of these is Entamoeba histolytica, which causes amebicdysentery.
MODEL SMZ-2T STEREOMICROSCOPE Model SMZ-2T Stereomicroscope. ( Circa 1987 ) The Nikon SMZ-2T microscope is a trinocular stereoscopic model that was designed to easily accept a closed-circuit television (CCTV) or newer CCD-based digital camera or any of Nikon's FX photomicrographic systems. A switchover control ring in the trinocular head allows the microscopistto rapidly
NIKON’S MUSEUM OF MICROSCOPY Nikon’s Museum of Microscopy. Nikon has been producing microscopes for almost a century, starting with a simple brass compound monocular brass microscope in the early 1900s. Today, Nikon microscopes are among the best in the World and are found in research laboratories in almost every country. The Nikon Museum of Microscopy features many of LIVE-CELL IMAGING CULTURE CHAMBERS A variety of Petri dish configurations specifically designed for high-resolution live-cell imaging are commercially available (see Figure 4). The simplest and perhaps most versatile design consists of a standard 35 or 50-millimeter disposable Petri dish featuring a circular opening (10, 14, or 20 millimeters) cut into the center of the attachment surface and covered by a high-qualityECLIPSE 80I
Eclipse 80i Upright Microscope. In response to Nikon’s ever-growing prominence in the cutting-edge research microscopy markets, Nikon introduced its all-new lineup of microscopes, the i-series. The 80i was one of the first in a family of very commercially successful upright microscopes that would help define the growth of the digitalera for
ECLIPSE E400
Eclipse E400. ( 1997 ) The Eclipse E400 biological microscope is Nikon's mid-level research instrument from the late 1990s. Though designed for stability, the instrument features a compact design, which was intended to help maintain a suitable comfort level for the microscopist over extended periods of NIKON PERFECT FOCUS SYSTEM (PFS) Nikon has addressed the problem of focus drift by creating a unique hardware solution, termed the Perfect Focus System (PFS), which is designed to combat axial focus fluctuations in real time during long-term imaging investigations.A wide range of dry, water, and oil immersion objectives, from 4x to 100x with varying numerical apertures and working distances, can be used with the TiE-PFS ADJUSTMENT OF OBJECTIVE CORRECTION COLLARS The tutorial initializes with a randomly selected specimen appearing in the Microscope Image window and a cut-away view of a correction collar objective positioned to the right of the image window. Also, upon initialization, the tutorial automatically selects a random cover glass thickness for the specimen being viewed in the Microscope Image window. . In order to operate the tutorial, use the LABOPHOT ENTRY-LEVEL RESEARCH MICROSCOPE Labophot Entry-Level Research Microscope. The Labophot was a sturdy, versatile microscope, produced in the 1980s. It offered research-level performance and a modular system that made it highly adaptable for clinical, biological, industrial, and educational laboratories. The Labophot utilized the Nikon CF optical system, which yielded highCCD NOISE SOURCES
CCD Noise Sources. Dark noise and read-out noise are two major sources of noise in CCD and other digital cameras for microscopy. Although great improvements have been made over the past few years in the reduction of CCD dark noise at room temperature, cooling the chip further reduces the noise tenfold per 20° C decrease.ECLIPSE 50/55I
Eclipse 50/55i. The Nikon Eclipse 50/55i was an important milestone in the evolution of Nikon’s clinical line of microscopes. Introduced in 2003, this microscope was a major step in incorporating a more comfortable, user-based design to allow people to sit and use the equipment for long hours without experiencing excessive discomfort orfatigue.
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Nikon Instruments | Nikon Global | Nikon Small World UPDATED Single-Molecule Super-Resolution Imaging NEW Light Sheet Fluorescence Microscopy Introduction to Fluorescent Proteins* 1
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WHAT'S NEW ON MICROSCOPYU We’re happy to announce that we’ve updated our article on Single-Molecule Super-Resolution Imaging. This article now contains an in-depth discussion of sample preparation, including near-comprehensive tables listing known fluorescent probes and buffer systems for single molecule localization microscopies.Learn More
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RESOLUTION
The resolving power of a microscope is the most important feature ofthe optical system.
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REFRACTIVE INDEX (INDEX OF REFRACTION) The ratio of the speed of light in a vacuum to that in the imaging medium of a microscope.*
INTRODUCTION TO POLARIZED LIGHT Discussion of birefringence, Brewster's angle, and various forms ofpolarized light.
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INTRODUCTION TO PHASE CONTRAST MICROSCOPY A mechanism to translate variations in phase into corresponding changes in amplitude. -------------------------View More
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SPATIAL RESOLUTION IN DIGITAL IMAGING Spatial resolution refers to the number of pixels utilized in construction of the image.*
STOCHASTIC OPTICAL RECONSTRUCTION MICROSCOPY (STORM) IMAGING Review the basic steps involved in acquiring a STORM super-resolutionimage.
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NUMERICAL APERTURE AND IMAGE RESOLUTION Explore how objective numerical aperture size influences Airy diskproperties.
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EYEPIECE RETICLE CALIBRATION Explores calibration of various eyepiece reticles using a stage micrometer and demonstrates how the reticle can then be employed to determine linear specimen dimensions. -------------------------View More
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MICHAEL DAVIDSON
Michael Davidson was a brilliant scientist, artist and photographer. The creator of MicroscopyU, Davidson also authored many scientific articles on the subject of photomicrography. His photomicrographs were published in more than a thousand national and international scientific journals, popular magazines and newspapers. In addition, Davidson’s photomicrography won more than 40 awards in scientific and industrial photography competitions and has been exhibited at over 50 locations nationwide.Learn more
2020 NIKON SMALL WORLD CALL FOR ENTRIESApril 30
is the deadline for photomicrographers, scientists, artists, and the public to submit images for the 2020 Nikon Small World Photomicrography and Small World in Motion competitions. * Enter 2020 Nikon Small World Competitions * View 2019 Nikon Small World Photomicrography Competition Winners * View 2019 Nikon Small World in Motion Competition Winners NIKON INSTRUMENTS WEBSITE HIGHLIGHTSNIS.ai
is a powerful new module for Nikon’s NIS-Elements imaging platform that enables you to: • Virtually stain with CONVERT.AI • Segment complex structures with SEGMENT.AI • Restore details to low-light images with ENHANCE.AI • Remove shot noise from resonant confocal images with DENOISE.AILearn more
------------------------- RECENT NEWS AND UPDATES NIKON BIOIMAGING LAB SYMPOSIUM October 30, 2019, via Nikon BioImaging Lab FLUORESCENT TURTLE EMBRYO WINS FORTY-FIFTH ANNUAL NIKON SMALL WORLDCOMPETITION
October 21, 2019, via Nikon Small World INVADOPODIA ARE CHEMOSENSING PROTRUSIONS THAT GUIDE CANCER CELL EXTRAVASATION TO PROMOTE BRAIN TROPISM IN METASTASIS January 18, 2019, via Nature GENETICALLY ENCODED FLUORESCENT INDICATORS FOR IMAGING INTRACELLULAR POTASSIUM ION CONCENTRATION January 17, 2019, via Nature Communications BiologySee all News
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* Microscopy Basics
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* Tutorials
* Galleries
* Cell Motility
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