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pathways
NCOUNTER® MIRNA EXPRESSION ASSAY USER MANUAL Introduction The nCounter miRNA Expression Assay is designed to provide an ultra-sensitive, reproducible, and highly multiplexed method for detecting miRNAs in total RNA across all biological levelsof
DEVELOPMENT OF AN INTERFERON SIGNATURE ON THE NANOSTRING Type I interferonopathies are characterized by an increase of circulating type I interferon (IFN) concentration. These diseases refer to rare Mendelian genetic disorders such as Aicardi-Goutières Syndrome (AGS) as well as more frequent and polygenic auto-immune diseases like systemic lupus erythematosus (SLE). GEOMX® WHOLE TRANSCRIPTOME ATLAS PRODUCT BULLETIN eoM hole ransriptome Atlas FOR RESEARCH USE ONLY. Not for use in diagnostic procedures. 3 GeoMx WTA Workflow GeoMx RNA assays contain in situ hybridization (ISH) probes conjugated to unique DNA indexing-oligonucleotides (DSP barcodes) via a UV-photocleavablelinker.
TECHNOLOGY FOR RESEARCH AND TRANSLATIONAL MEDICINEPRODUCTSPARTNERSHIPSSCIENTIFIC CONTENTNCOUNTER® SYSTEMSANALYSIS SOLUTIONS View Careers. “NanoString is a uniquely innovative place to work, as we get to do far more exciting and cutting-edge science than most companies our size. We experience the thrill of building technologies alongside the satisfaction that comes from participating in research advancing patient care for a broad range of disease areas.”. Erin NANOSTRING®: USER MANUAL NanoString® Technologies Inc. 530 Fairview Ave N Suite 2000 Seattle, Washington 98109 www.nanostring.com Tel: 206.378.6266 888.358.6266 E-mail: info@nanostring.com NCOUNTER® ADVANCED ANALYSIS SOFTWARE nSolver Advanced Analysis is a free, wizard-based add-on to nSolver™ Analysis Software for deeper data insights based on robust R statistics. Examine experimental trends, identify pathway-specific responses, and profile cell populations in sharable HTML reports. Support for all DNA, RNA and protein CodeSets, including custom reagents and panels. NSOLVER™ DATA ANALYSIS SUPPORT To view additional webinars on basic analysis, miRNA, CNV, Fusions, PlexSet, Protein analysis, and Advanced Analysis, check out our nSolver Webinar page to stream or download previously recorded sessions. For additional support regarding your nSolver analysis, please contact support@nanostring.com. GEOMX DIGITAL SPATIAL PROFILER (DSP) NanoString’s GeoMx Digital Spatial Profiler (DSP) combines the best of spatial and molecular profiling technologies by generating digital whole transcriptomes and profiling data for 100s of validated Protein analytes from up to 12 tissue slides per day. This unique combination of high-plex and high-throughput spatial profiling enables NCOUNTER® STEM CELL CHARACTERIZATION PANEL Deeply characterize and optimize your stem cell development with the nCounter Stem Cell Characterization Panel. This panel measures the eight essential components of stem cell biology. Evaluate viability, confirm functionality and determine pluripotency with a single robust, automated, and reproducible assay. NCOUNTER® INFLAMMATION PANEL Studying the early inflammatory response is fundamental to understanding the immune response and treating disease. The nCounter® Inflammation Panel lets you perform multiplex gene expression analysis on human or mouse samples with more than 200 genes focused on the study of inflammation. These genes represent a broad range of relevantpathways
NCOUNTER® MIRNA EXPRESSION ASSAY USER MANUAL Introduction The nCounter miRNA Expression Assay is designed to provide an ultra-sensitive, reproducible, and highly multiplexed method for detecting miRNAs in total RNA across all biological levelsof
DEVELOPMENT OF AN INTERFERON SIGNATURE ON THE NANOSTRING Type I interferonopathies are characterized by an increase of circulating type I interferon (IFN) concentration. These diseases refer to rare Mendelian genetic disorders such as Aicardi-Goutières Syndrome (AGS) as well as more frequent and polygenic auto-immune diseases like systemic lupus erythematosus (SLE). GEOMX® WHOLE TRANSCRIPTOME ATLAS PRODUCT BULLETIN eoM hole ransriptome Atlas FOR RESEARCH USE ONLY. Not for use in diagnostic procedures. 3 GeoMx WTA Workflow GeoMx RNA assays contain in situ hybridization (ISH) probes conjugated to unique DNA indexing-oligonucleotides (DSP barcodes) via a UV-photocleavablelinker.
NCOUNTER® LOW RNA INPUT KIT The nCounter Low RNA Input Kit enables the generation of high-quality gene expression profiling of up to 800 gene targets from as little as 1 ng of sample. The kit is optimized for use with RNA from Formalin-Fixed Paraffin-Embedded (FFPE) tissue as well as crude cell lysates. The streamlined, user friendly workflow enables geneexpression
NCOUNTER® STEM CELL CHARACTERIZATION PANEL Deeply characterize and optimize your stem cell development with the nCounter Stem Cell Characterization Panel. This panel measures the eight essential components of stem cell biology. Evaluate viability, confirm functionality and determine pluripotency with a single robust, automated, and reproducible assay. NCOUNTER® IMMUNOLOGY PANELS Perform multiplex gene expression analysis in human or mouse with over 500 general immunology genes. The nCounter Immunology Panel includes major classes of cytokines and their receptors, enzymes with specific gene families such as the major chemokine ligands and receptors, interferons and their receptors, the TNF-receptor superfamily, and the KIR family genes. 84 genes involved with the NCOUNTER® MIRNA EXPRESSION PANELS nCounter miRNA Expression panels are designed to provide a sensitive, reproducible, and highly multiplexed method for detecting miRNAs in total RNA across all biological levels of expression. The assay can be run on total RNA isolated from any source, including formalin-fixed paraffin embedded (FFPE) tissue sections. 01: NCOUNTER CONSUMABLES Each Master Kit order includes nCounter Cartridges, Prep Plates, and a Prep Pack, which includes racked tips and foil piercers, a 12-sample strip tube, strip tube caps, tube sheaths, cartridge well seals and hybridization buffer. Master Kits are available in packs of 12, 48 or 192 assays. A single nCounter Cartridge can process twelve samples. NCOUNTER® PANCANCER IMMUNE PROFILING PANEL Multi-analyte analysis with Vantage 3D™ Assays. The nCounter PanCancer Immune Profiling Panel is for cancer researchers that need more markers than is practical for RT-qPCR but don’t want the broad approach that next-gen sequencing (NGS) offers. The Panel is fully compatible with clinically relevant sample types such as fresh-frozen(FF
PROTEIN PROCESSING FOR FFPE SAMPLES Protein Processing for FFPE Samples MAN-10053-03 . 4 . FFPE Processing Recommendations . IMPORTANT: The antibody mix provided contains high levels of target oligonucleotide. CHARACTERIZING LATE-ONSET AD MODELS USING SPATIAL WHOLE REGISTER NOW Join NanoString and Jackson Laboratories to learn more about the latest spatial assay available now for shipment, the GeoMx®Mouse Whole
GEOMX® CANCER TRANSCRIPTOME ATLAS The GeoMx® Cancer Transcriptome Atlas (CTA) is designed for comprehensive profiling of tumor biology, the tumor microenvironment, and the immune response. Profile RNA expression of over 1,800 genes simultaneously with spatial resolution from distinct regions of interest with a single tissue section using the GeoMx Digital SpatialProfiler.
PANCANCER MOUSE IMMUNE PROFILING PANEL GENE LIST Catalyzing the next biological revolution to improve the human condition. Copyright © 2021 NanoString. All rights reserved Quick Links. Publications; Events TECHNOLOGY FOR RESEARCH AND TRANSLATIONAL MEDICINEPRODUCTSPARTNERSHIPSSCIENTIFIC CONTENTNCOUNTER® SYSTEMSANALYSIS SOLUTIONS View Careers. “NanoString is a uniquely innovative place to work, as we get to do far more exciting and cutting-edge science than most companies our size. We experience the thrill of building technologies alongside the satisfaction that comes from participating in research advancing patient care for a broad range of disease areas.”. Erin NANOSTRING®: USER MANUAL NanoString® Technologies Inc. 530 Fairview Ave N Suite 2000 Seattle, Washington 98109 www.nanostring.com Tel: 206.378.6266 888.358.6266 E-mail: info@nanostring.com NCOUNTER® STEM CELL CHARACTERIZATION PANEL Deeply characterize and optimize your stem cell development with the nCounter Stem Cell Characterization Panel. This panel measures the eight essential components of stem cell biology. Evaluate viability, confirm functionality and determine pluripotency with a single robust, automated, and reproducible assay. NCOUNTER® MIRNA EXPRESSION ASSAY USER MANUAL Introduction The nCounter miRNA Expression Assay is designed to provide an ultra-sensitive, reproducible, and highly multiplexed method for detecting miRNAs in total RNA across all biological levelsof
GEOMX® WHOLE TRANSCRIPTOME ATLAS PRODUCT BULLETIN eoM hole ransriptome Atlas FOR RESEARCH USE ONLY. Not for use in diagnostic procedures. 3 GeoMx WTA Workflow GeoMx RNA assays contain in situ hybridization (ISH) probes conjugated to unique DNA indexing-oligonucleotides (DSP barcodes) via a UV-photocleavablelinker.
NANOSTRING PANCANCER IO 360™ GENE EXPRESSION PANEL PanCancer IO 360™ Best Practices For Research Use Only NanoString Technologies® 2 LBL-10504-03 Tissue Processing The panel was developed for use on unstained FFPE slides 4–10 µm thick from archived tumor tissue PROTEIN PROCESSING FOR FFPE SAMPLES MAN-10053-03 Protein Processing for FFPE Samples . 5 . Primary Antibody Incubation CAUTION: Do not touch the tissue or allow it to dry throughout this process. NCOUNTER VANTAGE 3D GENE FUSION PANELS nCounter® Vantage 3D ™ Gene Fusion Panels Overview MAN-10085-01 . 2 . nCounter Fusion Chemistry NCOUNTER GENE EXPRESSION PANELS nCounter®Gene Expression Panels Overview MAN-10083-01 . 2 Product Workflow . Figure 2. Workflow for the nCounter Gene Expression Panels . Materials and Supporting Documents INTRODUCTION TO GEOMX CANCER TRANSCRIPTOME ATLAS 6| Introduction to GeoMx® Cancer Transcriptome Atlas: Normalization OCT 2020 FOR RESEARCH USE ONLY. Not for use in diagnostic procedures. Here is a visualization (Figure 6) of what the number of genes to be analyzed would look like based on different percentage AOI TECHNOLOGY FOR RESEARCH AND TRANSLATIONAL MEDICINEPRODUCTSPARTNERSHIPSSCIENTIFIC CONTENTNCOUNTER® SYSTEMSANALYSIS SOLUTIONS View Careers. “NanoString is a uniquely innovative place to work, as we get to do far more exciting and cutting-edge science than most companies our size. We experience the thrill of building technologies alongside the satisfaction that comes from participating in research advancing patient care for a broad range of disease areas.”. Erin NANOSTRING®: USER MANUAL NanoString® Technologies Inc. 530 Fairview Ave N Suite 2000 Seattle, Washington 98109 www.nanostring.com Tel: 206.378.6266 888.358.6266 E-mail: info@nanostring.com NCOUNTER® STEM CELL CHARACTERIZATION PANEL Deeply characterize and optimize your stem cell development with the nCounter Stem Cell Characterization Panel. This panel measures the eight essential components of stem cell biology. Evaluate viability, confirm functionality and determine pluripotency with a single robust, automated, and reproducible assay. NCOUNTER® MIRNA EXPRESSION ASSAY USER MANUAL Introduction The nCounter miRNA Expression Assay is designed to provide an ultra-sensitive, reproducible, and highly multiplexed method for detecting miRNAs in total RNA across all biological levelsof
GEOMX® WHOLE TRANSCRIPTOME ATLAS PRODUCT BULLETIN eoM hole ransriptome Atlas FOR RESEARCH USE ONLY. Not for use in diagnostic procedures. 3 GeoMx WTA Workflow GeoMx RNA assays contain in situ hybridization (ISH) probes conjugated to unique DNA indexing-oligonucleotides (DSP barcodes) via a UV-photocleavablelinker.
NANOSTRING PANCANCER IO 360™ GENE EXPRESSION PANEL PanCancer IO 360™ Best Practices For Research Use Only NanoString Technologies® 2 LBL-10504-03 Tissue Processing The panel was developed for use on unstained FFPE slides 4–10 µm thick from archived tumor tissue PROTEIN PROCESSING FOR FFPE SAMPLES MAN-10053-03 Protein Processing for FFPE Samples . 5 . Primary Antibody Incubation CAUTION: Do not touch the tissue or allow it to dry throughout this process. NCOUNTER VANTAGE 3D GENE FUSION PANELS nCounter® Vantage 3D ™ Gene Fusion Panels Overview MAN-10085-01 . 2 . nCounter Fusion Chemistry NCOUNTER GENE EXPRESSION PANELS nCounter®Gene Expression Panels Overview MAN-10083-01 . 2 Product Workflow . Figure 2. Workflow for the nCounter Gene Expression Panels . Materials and Supporting Documents INTRODUCTION TO GEOMX CANCER TRANSCRIPTOME ATLAS 6| Introduction to GeoMx® Cancer Transcriptome Atlas: Normalization OCT 2020 FOR RESEARCH USE ONLY. Not for use in diagnostic procedures. Here is a visualization (Figure 6) of what the number of genes to be analyzed would look like based on different percentage AOI SPATIAL MOLECULAR IMAGING OVERVIEW The current single-cell analysis technologies are applied to dissociated cells, which lose valuable information associated with cells anatomical and spatial contexts. NanoString’s Spatial Molecular Imaging technology enables the analysis of hundreds to thousands of RNAs or proteins directly from single cells, down to subcellular resolution NCOUNTER® STEM CELL CHARACTERIZATION PANEL Deeply characterize and optimize your stem cell development with the nCounter Stem Cell Characterization Panel. This panel measures the eight essential components of stem cell biology. Evaluate viability, confirm functionality and determine pluripotency with a single robust, automated, and reproducible assay. NCOUNTER® ADVANCED ANALYSIS SOFTWARE nSolver Advanced Analysis is a free, wizard-based add-on to nSolver™ Analysis Software for deeper data insights based on robust R statistics. Examine experimental trends, identify pathway-specific responses, and profile cell populations in sharable HTML reports. Support for all DNA, RNA and protein CodeSets, including custom reagents and panels. GEOMX MANUALS & GUIDES GeoMx Cancer Transcriptome Atlas. GeoMx Whole Transcriptome Atlas. Immune Pathways RNA assays. GeoMx Protein Assays. nCounter® AnalysisSystem. Show
NANOSTRING®: PRODUCT MANUAL NanoString Technologies, Inc. 530 Fairview Ave N Suite 2000 Seattle, Washington 98109 www.nanostring.com Tel: 206.378.6266 888.358.6266 E-mail: info@nanostring.com GEOMX DIGITAL SPATIAL PROFILER (DSP) NanoString’s GeoMx Digital Spatial Profiler (DSP) combines the best of spatial and molecular profiling technologies by generating digital whole transcriptomes and profiling data for 100s of validated Protein analytes from up to 12 tissue slides per day. This unique combination of high-plex and high-throughput spatial profiling enables NCOUNTER VANTAGE 3D GENE FUSION PANELS nCounter® Vantage 3D ™ Gene Fusion Panels Overview MAN-10085-01 . 2 . nCounter Fusion Chemistry NCOUNTER GENE EXPRESSION PANELS nCounter®Gene Expression Panels Overview MAN-10083-01 . 2 Product Workflow . Figure 2. Workflow for the nCounter Gene Expression Panels . Materials and Supporting Documents GENE EXPRESSION DATA ANALYSIS GUIDELINES Gene Expression Data Analysis Guidelines MAN-C0011-04 6 Limit of Detection QC The limit of detection is determined by measuring the ability to detect POS_E, the 0.5 fM positive control probe, which corresponds to about 10,000 copies of this target within each sampletube.
COPY NUMBER VARIATION ASSAY 2 PRODUCT BULLETIN nCounter® Copy Number Variation Assay R² = 0.9861 R² = 0.9709 R² = 0.9882 1 10 100 1000 10000 10 1001 0001 0000Normalized Counts
TECHNOLOGY FOR RESEARCH AND TRANSLATIONAL MEDICINEPRODUCTSPARTNERSHIPSSCIENTIFIC CONTENTNCOUNTER® SYSTEMSANALYSIS SOLUTIONS View Careers. “NanoString is a uniquely innovative place to work, as we get to do far more exciting and cutting-edge science than most companies our size. We experience the thrill of building technologies alongside the satisfaction that comes from participating in research advancing patient care for a broad range of disease areas.”. Erin NCOUNTER® ADVANCED ANALYSIS SOFTWAREADVANCED ANALYTICS CAPABILITIESADVANCED ANALYTICS CONFERENCEADVANCED AND PREDICTIVE ANALYTICS SOFTWARETOP 10 ANALYTICS SOFTWARE nSolver Advanced Analysis is a free, wizard-based add-on to nSolver™ Analysis Software for deeper data insights based on robust R statistics. Examine experimental trends, identify pathway-specific responses, and profile cell populations in sharable HTML reports. Support for all DNA, RNA and protein CodeSets, including custom reagents and panels. NANOSTRING®: USER MANUAL NanoString® Technologies Inc. 530 Fairview Ave N Suite 2000 Seattle, Washington 98109 www.nanostring.com Tel: 206.378.6266 888.358.6266 E-mail: info@nanostring.com NSOLVER™ DATA ANALYSIS SUPPORT To view additional webinars on basic analysis, miRNA, CNV, Fusions, PlexSet, Protein analysis, and Advanced Analysis, check out our nSolver Webinar page to stream or download previously recorded sessions. For additional support regarding your nSolver analysis, please contact support@nanostring.com. NCOUNTER® SPRINT PROFILER The nCounter SPRINT Profiler is a single instrument designed to let you digitally examine multiple pathways in a single tube and with an extraction-free workflow requiring only 10 minutes of hands-on time. Go from sample to answer inDetails
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